Human HVEM/TNFRSF14 Antibody

Catalog #: MAB3563 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB3563-100
MAB3563-SP

Detection of HVEM/TNFRSF14 in Human PBMC B Cells by Flow Cytometry.

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Human HVEM/TNFRSF14 Antibody Summary

Species Reactivity

Human

Specificity

Detects human HVEM/TNFRSF14 in ELISA.

Source

Recombinant Monoclonal Rabbit IgG Clone # 2742B

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Human embryonic kidney cell HEK293-derived human HVEM/TNFRSF14
Pro37-Val202
Accession # Q92956

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

Human PBMCs

Neutralization

In a functional ELISA binding assay, 0.075-0.45 ug/mL of this antibody will block 50% of the binding of 1 ug/mL of recombinant human BTLA His-tag protein (catalog# 9235-BT) to immobilized recombinant human HVEM Fc Chimera coat 1 ug/mL (100 µL/well). At 1 ug/mL, this antibody will block >90% of the binding.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of HVEM/TNFRSF14 in Human PBMC B Cells by Flow Cytometry. Human PBMC lymphocytes were stained with (A) Rabbit Anti-Human HVEM/TNFRSF14 Monoclonal Antibody (Catalog # MAB3563) or (B) isotype control antibody (MAB1050), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (F0111) and Mouse Anti-Human PE-conjugated CD19 (FAB4867P). Cells were gated on Mouse Anti-Human Alexa Fluor® 405-conjugated CD14 (FAB3832V), Mouse Anti-Human Alexa Fluor® 488-conjugated CD3 (FAB100G), and Mouse Anti-Human Alexa Fluor® 700-conjugated CD56 (FAB24086N) negative populations. Staining was performed using our Staining Membrane-associated Proteins protocol.

Flow Cytometry

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Detection of HVEM/TNFRSF14 in Human PBMC T cells by Flow Cytometry. Human PBMC lymphocytes were stained with (A) Rabbit Anti-Human HVEM/TNFRSF14 Monoclonal Antibody (Catalog # MAB3563) or (B) isotype control antibody (MAB1050), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (F0111) and Mouse Anti-Human Alexa Fluor® 488-conjugated CD3 (FAB100G). Cells were gated on Mouse Anti-Human Alexa Fluor® 405-conjugated CD14 (FAB3832V), Mouse Anti-Human PE-conjugated CD19 (FAB4867P), and Mouse Anti-Human Alexa Fluor® 700-conjugated CD56 (FAB24086N) negative populations. Staining was performed using our Staining Membrane-associated Proteins protocol.

Flow Cytometry

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Detection of HVEM/TNFRSF14 in Human PBMC NK cells by Flow Cytometry Human PBMC lymphocytes were stained with (A) Rabbit Anti-Human HVEM/TNFRSF14 Monoclonal Antibody (Catalog # MAB3563) or (B) isotype control antibody (MAB1050), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (F0111) and Mouse Anti-Human Alexa Fluor® 700-conjugated CD56 (FAB24086N). Cells were gated on Mouse Anti-Human Alexa Fluor® 405-conjugated CD14 (FAB3832V), Mouse Anti-Human PE-conjugated CD19 (FAB4867P), and Mouse Anti-Human Alexa Fluor® 488-conjugated CD3 (FAB100G) negative populations. Staining was performed using our Staining Membrane-associated Proteins protocol.

Flow Cytometry

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Detection of HVEM/TNFRSF14 in Human PBMC Monocytes by Flow Cytometry Human PBMC monocytes were stained with (A) Rabbit Anti-Human HVEM/TNFRSF14 Monoclonal Antibody (Catalog # MAB3563) or (B) isotype control antibody (MAB1050), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (F0111) and Mouse Anti-Human Alexa Fluor® 405-conjugated CD14 (FAB3832V). Cells were gated on Mouse Anti-Human Alexa Fluor® 488-conjugated CD3 (FAB100G), Mouse Anti-Human PE-conjugated CD19 (FAB4867P), and Mouse Anti-Human Alexa Fluor® 700-conjugated CD56 (FAB24086N) negative populations. Staining was performed using our Staining Membrane-associated Proteins protocol.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HVEM/TNFRSF14

HVEM (herpesvirus entry mediator), also known as TNFRSF14 and CD270, is a type I membrane protein in the TNF receptor superfamily, and it can both promote and inhibit T cell activity (1). Mature human HVEM consists of a 164 amino acid (aa) extracellular domain (ECD) with three cysteine-rich domains (CRD), a 21 aa transmembrane segment, and a 60 aa cytoplasmic tail with a TRAF interaction domain (2, 3). Within the ECD, human HVEM shares 55% aa sequence identity with mouse and rat HVEM. Alternative splicing generates an additional isoform with a substitution of the N-terminal 10 amino acids including the signal peptide. HVEM is highly expressed on naïve CD4⁺ T cells, CD8⁺ T memory cells, regulatory T cells, dendritic cells, monocytes, and neutrophils (4-8). Its expression declines during effector T cell activation but is up-regulated during Treg activation (4, 5). HVEM functions as a receptor for BTLA, CD160, LIGHT/TNFSF14, and Lymphotoxin-alpha (4, 9‑12). Ligation of HVEM by LIGHT triggers T cell, monocyte, and neutrophil activation (8, 10) and contributes to Th1 inflammation and cardiac allograft rejection (13, 14). In contrast, HVEM binding to CD160 or BTLA suppresses T cell and dendritic cell activation (4, 7, 9, 10) and dampens intestinal inflammation (15). HVEM enhances the development of CD8⁺ T cell memory and Treg function (5, 6). It is additionally expressed on intestinal epithelial cells, where its binding by intraepithelial lymphocyte (IEL) expressed CD160 promotes epitheilal integrity and host defense (16). The herpesvirus envelope glycoprotein gD, which binds HVEM to initiate membrane fusion, can antagonize both BTLA and LIGHT binding (2, 9, 11).

References

del Rio, M.L. et al. (2010) J. Leukoc. Biol. 87:223.
Montgomery, R.I. et al. (1996) Cell 87:427.
Hsu, H. et al. (1997) J. Biol. Chem. 272:13471.
Sedy, J. R. et al. (2005) Nat. Immunol. 6:90.
Tao, R. et al. (2008) J. Immunol. 180:6649.
Steinberg, M.W. et al. (2013) PLoS One 8:e77992.
de Trez, C. et al. (2008) J. Immunol. 180:238.
Heo, S.K. et al. (2006) J. Leukoc. Biol. 79:330.
Gonzalez, L.C. et al. (2005) Proc. Natl. Acad Sci. USA 102:1116.
Cai, G. et al. (2008) Nat. Immunol. 9:176.
Mauri, D.N. et al. (1998) Immunity 8:21.
Harrop, J.A. et al. (1998) J. Biol. Chem. 273:27548.
Wang, J. et al. (2005) J. Immunol. 174:8173.
Ye, Q. et al. (2002) J. Exp. Med. 195:795.
Steinberg, M.W. et al. (2008) J. Exp. Med. 205:1463.
Shui, J.W. et al. (2012) Nature 488:222.

Long Name

Herpesvirus Entry Mediator

Entrez Gene IDs

8764 (Human); 230979 (Mouse); 102137807 (Cynomolgus Monkey)

Alternate Names

ATAR; CD270 antigen; CD270; CD40-like protein; Herpes virus entry mediator A; Herpesvirus entry mediator A; HveA; HVEM; HVEMTR2HVEAATAR; LIGHTR; TNFRSF14; tumor necrosis factor receptor superfamily member 14; tumor necrosis factor receptor superfamily, member 14 (herpesvirus entrymediator); Tumor necrosis factor receptor-like 2; tumor necrosis factor receptor-like gene2