Human ICA1 Antibody

Catalog #: AF4084 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4084
AF4084-SP

Detection of ICA1 in A172 Human Cell Line by Flow Cytometry.

2 Images

All ICA1 Antibodies

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ICA1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human ICA1 in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human ICA1
Ser2-Ala482
Accession # ABW03414

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human ICA1

Immunohistochemistry

5-15 µg/mL

Immersion fixed paraffin-embedded sections of human pancreatic cancer tissue subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of ICA1 antibody in A172 Human Cell Line antibody by Flow Cytometry.

View Larger

Detection of ICA1 in A172 Human Cell Line by Flow Cytometry. A172 human glioblastoma cell line was stained with Goat Anti-Human ICA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4084, filled histo-gram) or isotype control antibody (AB-108-C, open histo-gram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (F0107). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Immunohistochemistry

View Larger

ICA1 in paraffin-embedded human pancreas. ICA1 was detected in immersion fixed paraffin-embedded sections of human pancreas using Goat Anti-Human ICA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4084) at 0.5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (red; Catalog # VC004) and counterstained with hematoxylin (blue). Specific staining was localized to islets of Langerhans.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ICA1

Islet Cell Autoantigen 1 (ICA1), also known as ICA 69 kDa (ICA69), is a cytosolic protein that is associated with the Golgi complex and with immature secretory granules. Its N-terminal region shares similarity with arfaptins (ADP-ribosylation factor-interacting-proteins), suggesting that ICA1 likely plays a role in membrane trafficking. Human ICA-1 is a 483 amino acids (aa) protein. As a result of aa insertions, deletions and substitutions, multiple isoforms exist. Human ICA-1 shows 89% and 94% aa identity to mouse and canine ICA-1, respectively.

Long Name

Islet Cell Autoantigen 1

Entrez Gene IDs

3382 (Human)

Alternate Names

diabetes mellitus type I autoantigen; ICA1; ICA69; ICAp69; ICAp69Islet cell autoantigen p69,69 kDa islet cell autoantigen; islet cell autoantigen 1 (69kD); islet cell autoantigen 1 isoform; islet cell autoantigen 1; islet cell autoantigen 1, 69kDa; p69