Human IFITM1 Antibody Summary
Met1-His36
Accession # NP_003632
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human IFITM1 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, TF-1 human erythroleukemic cell line, human placenta tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human IFITM1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4827) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for IFITM1 at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
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IFITM1 in K562 Human Cell Line. IFITM1 was detected in immersion fixed K562 human chronic myelogenous leukemia cell line using Goat Anti-Human IFITM1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4827) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IFITM1
IFITM1 (Interferon inducible transmembrane protein 1; also Leu13, CD225 and "9‑27") is a 17 kDa member of the CD225 family of proteins. It is inducible in multiple cell types by both type I and II interferons, and although reported to occur on the cell surface, is apparently concentrated in the ER. Homotypic IFITM1 interactions promote p53 antiproliferative activity by blocking p53 phosphorylation on Thr55. Human IFITM1 is a 2‑transmembrane protein 125 amino acids (aa) in length. The topology of the molecule is unclear, but transmembrane segments are reported between aa 37‑57 and 87‑107. Over aa 1‑36, human IFITM1 shares only 47% aa identity with mouse IFITM1.
Product Datasheets
Citations for Human IFITM1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Interferon-Induced Transmembrane Proteins Inhibit Infection by the Kaposi's Sarcoma-Associated Herpesvirus and the Related Rhesus Monkey Rhadinovirus in a Cell-Specific Manner
Authors: BF Hörnich, AK Gro beta kopf, CJ Dcosta, S Schlagowsk, AS Hahn
MBio, 2021-12-21;12(6):e0211321.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot -
?20 IFITM2 differentially restricts X4 and R5 HIV-1
Authors: WL Wu, CR Grotefend, MT Tsai, YL Wang, V Radic, H Eoh, IC Huang
Proc. Natl. Acad. Sci. U.S.A., 2017-06-19;114(27):7112-7117.
Species: Human
Sample Types: Recombinant Protein, Whole Cells
Applications: ICC, Western Blot -
Establishment of an Immortalized Endometriotic Stromal Cell Line from Human Ovarian Endometrioma
Authors: Yong Song, Niraj R. Joshi, Erin Vegter, Samantha Hrbek, Bruce A. Lessey, Asgerally T. Fazleabas
Reproductive Sciences
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