Human IFN-alpha 2 Quantikine HS ELISA Kit Summary
Product Summary
Precision
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 2.38 | 15.1 | 32.3 | 2.65 | 15.5 | 29.7 |
| Standard Deviation | 0.082 | 0.686 | 1.36 | 0.15 | 0.965 | 1.69 |
| CV% | 3.4 | 4.5 | 4.2 | 5.7 | 6.2 | 5.7 |
Recovery
The recovery of human IFN-alpha 2 spiked to levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 101 | 90-108 |
| EDTA Plasma (n=4) | 89 | 75-101 |
| Heparin Plasma (n=4) | 86 | 75-92 |
| Serum (n=4) | 86 | 75-99 |
Linearity
Scientific Data
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Human IFN-Alpha 2 High Sensitivity Quantikine Kit Spiked Linearity IFN-Alpha 2 is spiked at high concentration in various matrices and diluted with appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay. The linearity is between 87%-112% for Heparin Plasma, 97%-114% for EDTA Plasma, and 94%-111% for Serum.
Product Datasheets
Preparation and Storage
Background: IFN-alpha 2/IFNA2
Interferon-alpha (IFN-alpha), also known as leukocyte interferon, represents a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits, a 100 kDa ligand-binding subunit (IFN-alpha R2) and a 125 kDa ligand binding and signal transduction subunit (IFN-alpha R1) that is involved both in ligand binding and signal transduction. IFN-alpha has both anti-viral and immunomodulatory activities on target cells.
FAQs
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Assay Diluent RD1-63 contains visible precipitate. Is this acceptable to use?
A small amount of precipitate is expected and acceptable in Assay Diluent RD1-63. Variability has been observed between vials and diluent lots. It may not fully resolubilize but this will not impact assay performance. It is not recommended to filter the precipitate.
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