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Human IFN-beta Antibody

Catalog #: AF814
11 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF814
AF814-SP
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IFN‑ beta Inhibition of EMCV-induced Cytopathy and Neutralization by Human IFN-beta Antibody.
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Citations (11)
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Human IFN-beta Antibody Summary

Species Reactivity
Human
Specificity
Detects human IFN-beta in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human IFN-beta
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human IFN-beta
Neutralization
Measured by its ability to neutralize IFN‑ beta inhibition of EMCV-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129. The Neutralization Dose (ND50) is typically 0.100 - 1.00 µg/mL in the presence of 50 pg/mL Recombinant Human IFN‑ beta.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization IFN‑ beta Inhibition of EMCV-induced Cytopathy and Neutralization by Human IFN-beta Antibody. View Larger

IFN‑ beta Inhibition of EMCV-induced Cytopathy and Neutralization by Human IFN-beta Antibody. Recombinant Human IFN-beta (8499-IF) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of EMCV activity elicited by Recombinant Human IFN-beta (50 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IFN-beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF814). The ND50 is typically 0.100 - 1.00 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-beta

IFN-beta (interferon beta; also fibroblast IFN) is a secreted, monomeric 23-24 kDa member of the alpha/beta interferon family of molecules. It can be produced by almost all cell types in response to bacterial DNA or viral double-stranded RNA and is abundantly secreted by circulating plasmacytoid DCs. IFN-beta drives monocytic transformation into DCs, and it appears to stimulate a B cell switch from from IgM to IgG secretion. Mature human IFN-beta is 166 amino acids (aa) in length (aa 22-187) and contains one phosphorylation site at Ser140. There is one potential alternative start site at Met22. Full-length human IFN-beta (aa 22-187) shares 47% aa identity with mouse IFN-beta.

Long Name
Interferon beta
Entrez Gene IDs
3456 (Human); 15977 (Mouse); 24481 (Rat)
Alternate Names
Fibroblast interferon; IFB; IFBIFNB; IFF; IFNB; IFNB1; IFNbeta; IFN-beta; interferon beta; interferon, beta 1, fibroblast; MGC96956

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Product Specific Notices

Sold under license from Pestka Biomedical Laboratories, Inc. d/b/a PBL Interferon Source. For research use only. Not for resale. Not for diagnostic, therapeutic or clinical purposes.

Citations for Human IFN-beta Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. New World hantaviruses activate IFNlambda production in type I IFN-deficient vero E6 cells
    Authors: Joseph Prescott, Pamela Hall, Mariana Acuna-Retamar, Chunyan Ye, Marc G. Wathelet, Hideki Ebihara et al.
    PLoS ONE
  2. Sangassou Virus, the First Hantavirus Isolate from Africa, Displays Genetic and Functional Properties Distinct from Those of Other Murinae-Associated Hantaviruses
    Authors: Boris Klempa, Peter T. Witkowski, Elena Popugaeva, Brita Auste, Lamine Koivogui, Elisabeth Fichet-Calvet et al.
    Journal of Virology
  3. Data supporting the roles of BAP1, STING, and IFN-beta in ISGF3 activation in ccRCC
    Authors: LE Langbein, E Sementino, Z Zhong, W Jiang, L Li, JR Testa, H Yang
    Data in Brief, 2022-11-11;45(0):108743.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  4. Live imaging of neolymphangiogenesis identifies acute antimetastatic roles of dsRNA mimics
    Authors: D Olmeda, D Cerezo-Wal, C Mucientes, TG Calvo, E Cañón, D Alonso-Cur, N Ibarz, J Muñoz, JL Rodriguez-, P Ortiz-Rome, S Ortega, MS Soengas
    Embo Molecular Medicine, 2021-11-11;0(0):e12924.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  5. Dobrava-Belgrade hantavirus from Germany shows receptor usage and innate immunity induction consistent with the pathogenicity of the virus in humans.
    Authors: Popugaeva E, Witkowski PT, Schlegel M, Ulrich RG, Auste B, Rang A, Kruger DH, Klempa B
    PLoS ONE, 2012-04-24;7(4):e35587.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  6. Human cytomegalovirus IE1 protein elicits a type II interferon-like host cell response that depends on activated STAT1 but not interferon-gamma.
    Authors: Knoblach T, Grandel B, Seiler J, Nevels M, Paulus C
    PLoS Pathog., 2011-04-14;7(4):e1002016.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  7. The LIM-homeodomain transcription factor LMX1B regulates expression of NF-kappa B target genes.
    Authors: Rascle A, Neumann T, Raschta AS, Neumann A, Heining E, Kastner J, Witzgall R
    Exp. Cell Res., 2008-10-28;315(1):76-96.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  8. TLR3 can directly trigger apoptosis in human cancer cells.
    Authors: Salaun B, Coste I, Rissoan MC, Lebecque SJ, Renno T
    J. Immunol., 2006-04-15;176(8):4894-901.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  9. Inhibition of human immunodeficiency virus type 1 replication by Z-100, an immunomodulator extracted from human-type tubercle bacilli, in macrophages.
    Authors: Emori Y, Ikeda T, Ohashi T, Masuda T, Kurimoto T, Takei M, Kannagi M
    J. Gen. Virol., 2004-09-01;85(0):2603-13.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  10. PRC2-mediated epigenetic suppression of type I IFN-STAT2 signaling impairs antitumor immunity in luminal breast cancer
    Authors: J Hong, JH Lee, Z Zhang, Y Wu, M Yang, Y Liao, R de la Rosa, J Scheirer, D Pechacek, N Zhang, Z Xu, T Curiel, X Tan, TH Huang, K Xu
    Cancer Research, 2022-12-16;0(0):.
  11. Single-cell analysis of early antiviral gene expression reveals a determinant of stochastic IFNB1 expression
    Authors: Sultan Doğanay, Maurice Youzong Lee, Alina Baum, Jessie Peh, Sun-Young Hwang, Joo-Yeon Yoo et al.
    Integrative Biology

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