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Human IFN-beta Quantikine ELISA Kit

Catalog #: DIFNB0
28 Citations 4 Reviews Datasheet / COA / SDS
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Human IFN-beta Quantikine ELISA Spiked Recovery Competitor Comparision
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Citations (28)
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Reviews (4)
Summary Sample Values Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human IFN-beta Quantikine ELISA Kit Summary

Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL)
Sensitivity
0.781 pg/mL
Assay Range
7.8 - 500 pg/mL (Cell Culture Supernates)
Specificity
Natural and recombinant human IFN-beta.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Sample Values

Cell Culture Supernates: A549 human lung carcinoma cells were cultured in Kaighn's Nutrient Mixture F-12 supplemented with 10% fetal bovine serum until nearly confluent. The cells were cultured unstimulated or stimulated with 10 μg/mL of poly I:C in the presence of Lipofectamine 2000 (LF2K) for 24 hours. Aliquots of the cell culture supernates were removed and assayed for levels of human IFN-beta.

Conditionpg/mL
UnstimulatedND
Stimulated848
ND=Non-detectable

CCD-1070Sk human foreskin fibroblast cells were cultured in MEM NEAA Earle's Salts supplemented with 10% fetal bovine serum until nearly confluent. The cells were cultured unstimulated or stimulated with 10 μg/mL of poly I:C in the presence of Lipofectamine 2000 (LF2K) for 24 hours. Aliquots of the cell culture supernates were removed and assayed for levels of human IFN-beta.

Conditionpg/mL
UnstimulatedND
Stimulated2323
ND=Non-detectable


Product Summary

The Quantikine® Human IFN-beta Immunoassay is a 4.5 hour solid phase ELISA designed to measure human IFN-beta in cell culture supernates. It contains CHO cell-expressed recombinant human IFN-beta and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IFN-beta showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IFN-beta.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Cell Culture Supernates

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 58.3 159 295 56.4 160 275
Standard Deviation 1.71 6.01 13.8 5.26 13 21.9
CV% 2.9 3.8 4.7 9.3 8.1 8

Recovery

The recovery of human IFN-beta spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=5) 107 95-111

Linearity

To assess the linearity of the assay, samples containing high concentrations of human IFN-beta were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Human IFN-beta ELISA Linearity

Scientific Data

ELISA Human IFN-beta Quantikine ELISA Spiked Recovery Competitor Comparision View Larger

Human IFN-beta Quantikine ELISA Spiked Recovery Competitor Comparision IFN-beta is spiked at three known concentrations throughout the range of the assay and run to measure response of the spiked sample matrix. R&D Systems recovery is 98% compared to 55% for the top competitor.

ELISA Human IFN-beta Quantikine ELISA Natural Linearity Competitor Comparison View Larger

Human IFN-beta Quantikine ELISA Natural Linearity Competitor Comparison Samples containing high concentrations of IFN-beta in various matrices are diluted with appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay. R&D Systems linearity is between 103%-112% compared to 119%-170% for the top competitor.

N/A Human IFN- beta ELISA Standard Curve View Larger

Human IFN- beta ELISA Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IFN-beta

Interferon-beta (IFN-beta) is a cytokine that is produced by fibroblasts and pathogen-exposed dendritic cells, macrophages, and endothelial cells. It signals through the heterodimeric IFN-alpha/beta Receptor. IFN-beta-deficient mice show increased susceptibility to experimental autoimmune encephalomyelitis (EAE), a disease model of human multiple sclerosis (MS). Furthermore, IFN-beta has been shown to suppress the Th17 cell response in both MS and EAE and has commonly been used as a treatment for MS.

Long Name:
Interferon beta
Entrez Gene IDs:
3456 (Human); 15977 (Mouse); 24481 (Rat)
Alternate Names:
Fibroblast interferon; IFB; IFBIFNB; IFF; IFNB; IFNB1; IFNbeta; IFN-beta; interferon beta; interferon, beta 1, fibroblast; MGC96956
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human IFN-beta Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

28 Citations: Showing 1 - 10
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  1. Helicase protein DDX11 as a novel antiviral factor promoting RIG-I-MAVS-mediated signaling pathway
    Authors: Zhang, J;Zhang, L;Liu, D;Shi, H;Zhang, X;Chen, J;Yang, X;Zeng, M;Zhang, J;Feng, T;Zhu, X;Jing, Z;Ji, Z;Shi, D;Feng, L;
    mBio
    Species: Human
    Sample Types: Cell Culture Supernates
  2. Repression of mRNA translation initiation by GIGYF1 via disrupting the eIF3-eIF4G1 interaction
    Authors: Choi, JH;Luo, J;Hesketh, GG;Guo, S;Pistofidis, A;Ladak, RJ;An, Y;Naeli, P;Alain, T;Schmeing, TM;Gingras, AC;Duchaine, T;Zhang, X;Sonenberg, N;Jafarnejad, SM;
    Science advances
    Species: Human
    Sample Types: Cell Culture Supernates
  3. Major Group-B Enterovirus populations deleted in the noncoding 5' region of genomic RNA modulate activation of the type I interferon pathway in cardiomyocytes and induce myocarditis
    Authors: Callon, D;Glenet, M;Lebreil, AL;Heng, L;Bouland, N;Fichel, C;Fornes, P;Andreoletti, L;Berri, F;
    PLoS pathogens
    Species: Mouse
    Sample Types: Cell Culture Supernates
  4. Oxygen enhances antiviral innate immunity through maintenance of EGLN1-catalyzed proline hydroxylation of IRF3
    Authors: Liu, X;Tang, J;Wang, Z;Zhu, C;Deng, H;Sun, X;Yu, G;Rong, F;Chen, X;Liao, Q;Jia, S;Liu, W;Zha, H;Fan, S;Cai, X;Gui, JF;Xiao, W;
    Nature communications
    Species: Human
    Sample Types: Cell Culture Supernates
  5. The inhibitory effect of DIF-3 on polyinosinic-polycytidylic acid-induced innate immunity activation in human cerebral microvascular endothelial cells
    Authors: Araya, R;Men, S;Uekusa, Y;Yu, Z;Kikuchi, H;Daitoku, K;Minakawa, M;Kawaguchi, S;Furukawa, KI;Oshima, Y;Imaizumi, T;Seya, K;
    Journal of pharmacological sciences
    Species: Human
    Sample Types: Cell Culture Supernates
  6. Evolution of enhanced innate immune suppression by SARS-CoV-2 Omicron subvariants
    Authors: Reuschl, AK;Thorne, LG;Whelan, MVX;Ragazzini, R;Furnon, W;Cowton, VM;De Lorenzo, G;Mesner, D;Turner, JLE;Dowgier, G;Bogoda, N;Bonfanti, P;Palmarini, M;Patel, AH;Jolly, C;Towers, GJ;
    Nature microbiology
    Species: Human
    Sample Types: Cell Culture Supernates
  7. Targeting DHX9 triggers tumor-intrinsic interferon response and replication stress in Small Cell Lung Cancer
    Authors: Murayama, T;Nakayama, J;Jiang, X;Miyata, K;Morris, AD;Cai, KQ;Prasad, RM;Ma, X;Efimov, A;Belani, N;Gerstein, ER;Tan, Y;Zhou, Y;Kim, W;Maruyama, R;Campbell, KS;Chen, L;Yang, Y;Balachandran, S;Canadas, I;
    Cancer discovery
    Species: Human
    Sample Types: Cell Culture Supernates
  8. Macrophage fumarate hydratase restrains mtRNA-mediated interferon production
    Authors: A Hooftman, CG Peace, DG Ryan, EA Day, M Yang, AF McGettrick, M Yin, EN Montano, L Huo, JE Toller-Kaw, V Zecchini, TAJ Ryan, A Bolado-Car, AM Casey, HA Prag, ASH Costa, G De Los San, M Ishimori, DJ Wallace, S Venuturupa, E Nikitopoul, N Frizzell, C Johansson, A Von Kriegs, MP Murphy, C Jefferies, C Frezza, LAJ O'Neill
    Nature, 2023-03-08;615(7952):490-498.
    Species: Human
    Sample Types: Cell Culture Supernates
  9. TRIM21 inhibits irradiation-induced mitochondrial DNA release and impairs antitumour immunity in nasopharyngeal carcinoma tumour models
    Authors: JY Li, Y Zhao, S Gong, MM Wang, X Liu, QM He, YQ Li, SY Huang, H Qiao, XR Tan, ML Ye, XH Zhu, SW He, Q Li, YL Liang, KL Chen, SW Huang, QJ Li, J Ma, N Liu
    Nature Communications, 2023-02-16;14(1):865.
    Species: Human
    Sample Types: Cell Culture Supernates
  10. Telomere-to-mitochondria signalling by ZBP1 mediates replicative crisis
    Authors: J Nassour, LG Aguiar, A Correia, TT Schmidt, L Mainz, S Przetocka, C Haggblom, N Tadepalle, A Williams, MN Shokhirev, SC Akincilar, V Tergaonkar, GS Shadel, J Karlseder
    Nature, 2023-02-08;614(7949):767-773.
    Species: Human
    Sample Types: Cell Culture Supernates
  11. 2'-5' oligoadenylate synthetase?like 1 (OASL1) protects against atherosclerosis by maintaining endothelial nitric oxide synthase mRNA stability
    Authors: TK Kim, S Jeon, S Park, SK Sonn, S Seo, J Suh, J Jin, HY Kweon, S Kim, SH Moon, O Kweon, BH Koo, N Kim, HO Lee, YM Kim, YJ Kim, SH Park, GT Oh
    Nature Communications, 2022-11-04;13(1):6647.
    Species: Human, Mouse
    Sample Types: Cell Culture Supernates, Plasma
  12. Lactobacillus crispatus Limits Bladder Uropathogenic E. coli Infection by Triggering a Host Type I Interferon Response
    Authors: CH Song, YH Kim, M Naskar, BW Hayes, MA Abraham, JH Noh, G Suk, MJ Kim, KS Cho, M Shin, EJ Lee, SN Abraham, HW Choi
    Proceedings of the National Academy of Sciences of the United States of America, 2022-08-08;119(33):e2117904119.
    Species: Human
    Sample Types: Cell Culture Supernates
  13. Pharmacological Activation of STING in Bladder Induces Potent Anti-tumor Immunity in Non-Muscle Invasive Murine Bladder Cancer
    Authors: KC Huang, D Chanda, S McGrath, V Dixit, C Zhang, J Wu, K Tendyke, H Yao, R Hukkanen, N Taylor, D Verbel, DS Kim, A Endo, TA Noland, Y Chen, M Matijevic, J Wang, J Hutz, N Sarwar, FG Fang, X Bao
    Molecular Cancer Therapeutics, 2022-06-01;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  14. SHR1032, a novel STING agonist, stimulates anti-tumor immunity and directly induces AML apoptosis
    Authors: C Song, D Liu, S Liu, D Li, I Horecny, X Zhang, P Li, L Chen, M Miller, R Chowdhury, M Issa, R Shen, Y Yan, F Zhang, L Zhang, L Zhang, C Bai, J Feng, L Zhuang, R Zhang, J Li, H Wilkinson, J Liu, W Tao
    Scientific Reports, 2022-05-20;12(1):8579.
    Species: Cynomolgus Monkey, Human
    Sample Types: Cell Culture Supernates
  15. A yellow fever virus NS4B inhibitor not only suppresses viral replication, but also enhances the virus activation of RIG-I-like receptor-mediated innate immune response
    Authors: Z Gao, X Zhang, L Zhang, S Wu, J Ma, F Wang, Y Zhou, X Dai, E Bullitt, Y Du, JT Guo, J Chang
    PloS Pathogens, 2022-01-21;18(1):e1010271.
    Species: Human
    Sample Types: Cell Culture Supernates
  16. Mayaro Virus Non-Structural Protein 2 Circumvents the Induction of Interferon in Part by Depleting Host Transcription Initiation Factor IIE Subunit 2
    Authors: R Ishida, J Cole, J Lopez-Oroz, N Fayad, A Felix-Lope, M Elaish, SY Luo, O Julien, A Kumar, TC Hobman
    Cells, 2021-12-12;10(12):.
    Species: Human
    Sample Types: Cell Culture Supernates
  17. Human cytomegalovirus blocks canonical TGFbeta signaling during lytic infection to limit induction of type I interferons
    Authors: AH Pham, J Mitchell, S Botto, KM Pryke, VR DeFilippis, MH Hancock
    PloS Pathogens, 2021-08-19;17(8):e1009380.
    Species: Human
    Sample Types: Cell Culture Supernates
  18. Antiviral Activity of Influenza A Virus Defective Interfering Particles against SARS-CoV-2 Replication In Vitro through Stimulation of Innate Immunity
    Authors: U Rand, SY Kupke, H Shkarlet, MD Hein, T Hirsch, P Marichal-G, L Cicin-Sain, U Reichl, D Bruder
    Cells, 2021-07-11;10(7):.
    Species: Human
    Sample Types: Cell Culture Supernates
  19. G-quadruplex binders as cytostatic modulators of innate immune genes in cancer cells
    Authors: G Miglietta, M Russo, RC Duardo, G Capranico
    Nucleic Acids Research, 2021-07-09;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  20. Plasma-derived DNA containing-extracellular vesicles induce STING-mediated proinflammatory responses in dermatomyositis
    Authors: Y Li, C Bax, J Patel, T Vazquez, A Ravishanka, MM Bashir, M Grinnell, D Diaz, VP Werth
    Theranostics, 2021-05-21;11(15):7144-7158.
    Species: Human
    Sample Types: Cell Culture Supernates
  21. Thapsigargin Is a Broad-Spectrum Inhibitor of Major Human Respiratory Viruses: Coronavirus, Respiratory Syncytial Virus and Influenza A Virus
    Authors: S Al-Beltagi, CA Preda, LV Goulding, J James, J Pu, P Skinner, Z Jiang, BL Wang, J Yang, AC Banyard, KH Mellits, P Gershkovic, CJ Hayes, J Nguyen-Van, IH Brown, J Liu, KC Chang
    Viruses, 2021-02-03;13(2):.
    Species: Human
    Sample Types: Cell Culture Supernates
  22. PPM1G restricts innate immune signaling mediated by STING and MAVS and is hijacked by KSHV for immune evasion
    Authors: K Yu, H Tian, H Deng
    Sci Adv, 2020-11-20;6(47):.
    Species: Human
    Sample Types: Cell Culture Supernates
  23. Frontline Science: AMPK regulates metabolic reprogramming necessary for interferon production in human plasmacytoid dendritic cells
    Authors: HJ Hurley, H Dewald, ZS Rothkopf, S Singh, F Jenkins, P Deb, S De, BJ Barnes, P Fitzgerald
    J. Leukoc. Biol., 2020-07-08;0(0):.
    Species: Human
    Sample Types: cell culture supernatant
  24. MTFMT deficiency correlates with reduced mitochondrial integrity and enhanced host susceptibility to intracellular infection
    Authors: JH Seo, CS Hwang, JY Yoo
    Sci Rep, 2020-07-07;10(1):11183.
    Species: Human
    Sample Types: Cell Culture Supernates
  25. ILF3 contributes to the establishment of the antiviral type I interferon program
    Authors: SF Watson, N Bellora, S Macias
    Nucleic Acids Res., 2020-01-10;0(0):.
    Species: Human
    Sample Types: Cell Culture Superantes
  26. Mitochondria-localised ZNFX1 functions as a dsRNA sensor to initiate antiviral responses through MAVS
    Authors: Y Wang, S Yuan, X Jia, Y Ge, T Ling, M Nie, X Lan, S Chen, A Xu
    Nat. Cell Biol., 2019-11-04;21(11):1346-1356.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  27. STING Signaling Promotes Inflammation in Experimental Acute Pancreatitis
    Authors: Q Zhao, Y Wei, SJ Pandol, L Li, A Habtezion
    Gastroenterology, 2018-02-06;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  28. Reduced-HMGB1 suppresses poly(I:C)-induced inflammation in keratinocytes
    Authors: H Mori, M Murakami, T Tsuda, K Kameda, R Utsunomiya, K Masuda, K Shiraishi, X Dai, M Tohyama, H Nakaoka, K Sayama
    J. Dermatol. Sci., 2018;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Capture

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Reviews for Human IFN-beta Quantikine ELISA Kit

Average Rating: 4.8 (Based on 4 Reviews)

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Human IFN-beta Quantikine ELISA Kit
By Anonymous on 10/14/2023
Sample Tested: A549 human lung carcinoma cell line,HCC827 human non-small cell lung cancer cell line,Lung cancer tissue

The test kit's results are as expected! We are very excited. We examined various lung cancer cell lines for immune cell interventions and the TME components of primary cultured tissues.

Human IFN-beta Quantikine ELISA Kit DIFNB0
Human IFN-beta Quantikine ELISA Kit
By Thanh Nguyen on 10/18/2020
Sample Tested: Blood B cells
Human IFN-beta Quantikine ELISA Kit
By Anonymous on 12/18/2019
Sample Tested: Monocyte-derived dendritic cells

Human Monocyte-Derived Dendritic cells infected with a lentivirus. 2x diluted cell culture supernatants.

Human IFN-beta Quantikine ELISA Kit DIFNB0
Human IFN-beta Quantikine ELISA Kit
By Anonymous on 08/06/2019
Sample Tested: Jurkat human acute T cell leukemia cell line