Human IFN-gamma PE-conjugated Antibody Summary
Gln24-Gln166
Accession # AAP20098.1
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
Detection of IFN‑ gamma in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with 50 ng/mL PMA, 1 ug/mL Ionomycin, and 3 uM Monensin overnight were stained with Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (Catalog # FAB100A) and either (A) Mouse Anti-Human IFN-gamma PE-conjugated Monoclonal Antibody (Catalog # IC285P) or (B) Mouse IgG2BPhycoerythrin Isotype Control (Catalog # IC0041P). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IFN-gamma
Interferon-gamma (IFN-gamma, IFNG), also known as type II or Immune Interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine. Mature human IFN-gamma exists as a non-covalently linked homodimer of 20-25 kDa molecular weight variably glycosylated subunits. Glycosylation of IFN-gamma at sites Asn25 and Asn97 is critical for protease resistance. It shares 90% amino acid (aa) sequence identity with rhesus IFN-gamma, 59-64% with bovine, canine, equine, feline, and porcine IFN‑ gamma, and 37-43% with cotton rat, mouse, and rat IFN-gamma. IFN-gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction. IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. It plays a key function in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, up‑regulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects. In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation. The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis.
Product Datasheets
Citations for Human IFN-gamma PE-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
19
Citations: Showing 1 - 10
Filter your results:
Filter by:
-
Gain-of-function human STAT1 mutations impair IL-17 immunity and underlie chronic mucocutaneous candidiasis
Authors: Luyan Liu, Satoshi Okada, Xiao-Fei Kong, Alexandra Y. Kreins, Sophie Cypowyj, Avinash Abhyankar et al.
Journal of Experimental Medicine
-
Early T cell and binding antibody responses are associated with COVID-19 RNA vaccine efficacy onset
Authors: Shirin Kalimuddin, Christine Y.L. Tham, Martin Qui, Ruklanthi de Alwis, Jean X.Y. Sim, Joey M.E. Lim et al.
Med (N Y)
-
Alterations of the NK cell pool in HIV/HCV co-infection
Authors: DJ Kaczmarek, P Kokordelis, B Krämer, A Glässner, F Wolter, F Goeser, P Lutz, C Schwarze-Z, C Boesecke, CP Strassburg, JK Rockstroh, U Spengler, J Nattermann
PLoS ONE, 2017-04-05;12(4):e0174465.
-
Preconditioned Chorionic Villus Mesenchymal Stem/Stromal Cells (CVMSCs) Minimize the Invasive Phenotypes of Breast Cancer Cell Line MDA231 In Vitro
Authors: Subayyil, AA;Basmaeil, YS;Kulayb, HB;Alrodayyan, M;Alhaber, LAA;Almanaa, TN;Khatlani, T;
International journal of molecular sciences
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Dissection of the NKG2C NK cell response against Puumala Orthohantavirus
Authors: H Vietzen, S Hartenberg, SW Aberle, E Puchhammer
PloS Neglected Tropical Diseases, 2021-12-06;15(12):e0010006.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Influence of a High-Impact Multidimensional Rehabilitation Program on the Gut Microbiota of Patients with Multiple Sclerosis
Authors: M Barone, L Mendozzi, F D'Amico, M Saresella, S Rampelli, F Piancone, F La Rosa, I Marventano, M Clerici, A d'Arma, L Pugnetti, V Rossi, M Candela, P Brigidi, S Turroni
International Journal of Molecular Sciences, 2021-07-02;22(13):.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Early induction of functional SARS-CoV-2-specific T cells associates with rapid viral clearance and mild disease in COVID-19 patients
Authors: AT Tan, M Linster, CW Tan, N Le Bert, WN Chia, K Kunasegara, Y Zhuang, CYL Tham, A Chia, GJD Smith, B Young, S Kalimuddin, JGH Low, D Lye, LF Wang, A Bertoletti
Cell Reports, 2021-01-21;34(6):108728.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Serum microRNA181a: Correlates with the intracellular cytokine levels and a potential biomarker for acute graft-versus-host disease
Authors: Linna Xie
Cytokine, 2016-06-08;85(0):37-44.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Inherited IL-17RC deficiency in patients with chronic mucocutaneous candidiasis.
Authors: Ling Y, Cypowyj S, Aytekin C, Galicchio M, Camcioglu Y, Nepesov S, Ikinciogullari A, Dogu F, Belkadi A, Levy R, Migaud M, Boisson B, Bolze A, Itan Y, Goudin N, Cottineau J, Picard C, Abel L, Bustamante J, Casanova J, Puel A
J Exp Med, 2015-04-27;212(5):619-31.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Comparative Multi-Donor Study of IFNgamma Secretion and Expression by Human PBMCs Using ELISPOT Side-by-Side with ELISA and Flow Cytometry Assays.
Authors: Hagen J, Zimmerman R, Goetz C, Bonnevier J, Houchins J, Reagan K, Kalyuzhny A
Cells, 2015-02-11;4(1):84-95.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
High-content cytometry and transcriptomic biomarker profiling of human B-cell activation.
Authors: Hennig C, Ilginus C, Boztug K, Skokowa J, Marodi L, Szaflarska A, Sass M, Pignata C, Kilic S, Caragol I, Baumann U, Klein C, Welte K, Hansen G
J Allergy Clin Immunol, 2013-09-05;133(1):172-80.e1-10.
Species: Human
Sample Types: Whole Cells
Applications: Chip Cytometry -
Conditional ligands for Asian HLA variants facilitate the definition of CD8+ T-cell responses in acute and chronic viral diseases.
Authors: Chang C, Tan A, Or M, Toh K, Lim P, Chia A, Froesig T, Nadua K, Oh H, Leong H, Hadrup S, Gehring A, Tan Y, Bertoletti A, Grotenbreg G
Eur J Immunol, 2013-02-14;43(4):1109-20.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
NK cells from HCV-infected patients effectively induce apoptosis of activated primary human hepatic stellate cells in a TRAIL-, FasL- and NKG2D-dependent manner.
Authors: Glassner A, Eisenhardt M, Kramer B, Korner C, Coenen M, Sauerbruch T, Spengler U, Nattermann J
Lab. Invest., 2012-03-26;92(7):967-77.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Cross-presentation of tumour antigens by human induced pluripotent stem cell-derived CD141(+)XCR1+ dendritic cells.
Gene Ther, 2011-11-10;19(10):1035-40.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Identification of human NK17/NK1 cells.
Authors: Pandya AD, Al-Jaderi Z, Hoglund RA, Holmoy T, Harbo HF, Norgauer J, Maghazachi AA
PLoS ONE, 2011-10-21;6(10):e26780.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Human CD4(+) T cells recognize an epitope within alpha-fetoprotein sequence and develop into TGF-beta-producing CD4(+) T cells.
Authors: Alisa A, Boswell S, Pathan AA, Ayaru L, Williams R, Behboudi S
J. Immunol., 2008-04-01;180(7):5109-17.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
A reproducible method for the enumeration of functional (cytokine producing) versus non-functional peptide-specific cytotoxic T lymphocytes in human peripheral blood.
Authors: Markovic SN, Nevala WK, Uhl CB, Celis E, McKean DJ
Clin. Exp. Immunol., 2006-09-01;145(3):438-47.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Plasma cell-like morphology of Th1-cytokine-producing cells associated with the loss of CD3 expression.
Authors: Page G, Sattler A, Kersten S, Thiel A, Radbruch A, Miossec P
Am. J. Pathol., 2004-02-01;164(2):409-17.
Species: Human
Sample Types: Whole Cells, Whole Tissue
Applications: ICC, IHC-P -
Fatty Acid Synthase Contributes to Restimulation-Induced Cell Death of Human CD4 T Cells
Authors: Kelsey Voss, Christopher R. Luthers, Katherine Pohida, Andrew L. Snow
Frontiers in Molecular Biosciences
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Human IFN-gamma PE-conjugated Antibody
There are currently no reviews for this product. Be the first to review Human IFN-gamma PE-conjugated Antibody and earn rewards!
Have you used Human IFN-gamma PE-conjugated Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image