Human IFN-gamma PE-conjugated Antibody

Catalog # Availability Size / Price Qty
IC285P-100
Detection of IFN‑ gamma  in Human PBMCs by Flow Cytometry.
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Product Details
Citations (19)
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Human IFN-gamma PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human IFN-gamma in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse IFN-gamma, recombinant rat IFN-gamma, or recombinant porcine IFN-gamma is observed.
Source
Monoclonal Mouse IgG2B Clone # 25723
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human IFN-gamma
Gln24-Gln166
Accession # AAP20098.1
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of IFN-? antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of IFN‑ gamma in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with 50 ng/mL PMA, 1 ug/mL Ionomycin, and 3 uM Monensin overnight were stained with Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (Catalog # FAB100A) and either (A) Mouse Anti-Human IFN-gamma PE-conjugated Monoclonal Antibody (Catalog # IC285P) or (B) Mouse IgG2BPhycoerythrin Isotype Control (Catalog # IC0041P). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IFN-gamma

Interferon-gamma (IFN-gamma, IFNG), also known as type II or Immune Interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine. Mature human IFN-gamma exists as a non-covalently linked homodimer of 20-25 kDa molecular weight variably glycosylated subunits. Glycosylation of IFN-gamma at sites Asn25 and Asn97 is critical for protease resistance. It shares 90% amino acid (aa) sequence identity with rhesus IFN-gamma, 59-64% with bovine, canine, equine, feline, and porcine IFN‑ gamma, and 37-43% with cotton rat, mouse, and rat IFN-gamma. IFN-gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction. IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. It plays a key function in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, up‑regulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects. In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation. The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis.

Long Name
Interferon gamma
Entrez Gene IDs
3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline)
Alternate Names
IFG; IFI; IFNG; IFNgamma; IFN-gamma; Immune interferon; interferon gamma; interferon, gamma

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Citations for Human IFN-gamma PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

19 Citations: Showing 1 - 10
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  1. Gain-of-function human STAT1 mutations impair IL-17 immunity and underlie chronic mucocutaneous candidiasis
    Authors: Luyan Liu, Satoshi Okada, Xiao-Fei Kong, Alexandra Y. Kreins, Sophie Cypowyj, Avinash Abhyankar et al.
    Journal of Experimental Medicine
  2. Early T cell and binding antibody responses are associated with COVID-19 RNA vaccine efficacy onset
    Authors: Shirin Kalimuddin, Christine Y.L. Tham, Martin Qui, Ruklanthi de Alwis, Jean X.Y. Sim, Joey M.E. Lim et al.
    Med (N Y)
  3. Alterations of the NK cell pool in HIV/HCV co-infection
    Authors: DJ Kaczmarek, P Kokordelis, B Krämer, A Glässner, F Wolter, F Goeser, P Lutz, C Schwarze-Z, C Boesecke, CP Strassburg, JK Rockstroh, U Spengler, J Nattermann
    PLoS ONE, 2017-04-05;12(4):e0174465.
  4. Preconditioned Chorionic Villus Mesenchymal Stem/Stromal Cells (CVMSCs) Minimize the Invasive Phenotypes of Breast Cancer Cell Line MDA231 In Vitro
    Authors: Subayyil, AA;Basmaeil, YS;Kulayb, HB;Alrodayyan, M;Alhaber, LAA;Almanaa, TN;Khatlani, T;
    International journal of molecular sciences
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Dissection of the NKG2C NK cell response against Puumala Orthohantavirus
    Authors: H Vietzen, S Hartenberg, SW Aberle, E Puchhammer
    PloS Neglected Tropical Diseases, 2021-12-06;15(12):e0010006.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Influence of a High-Impact Multidimensional Rehabilitation Program on the Gut Microbiota of Patients with Multiple Sclerosis
    Authors: M Barone, L Mendozzi, F D'Amico, M Saresella, S Rampelli, F Piancone, F La Rosa, I Marventano, M Clerici, A d'Arma, L Pugnetti, V Rossi, M Candela, P Brigidi, S Turroni
    International Journal of Molecular Sciences, 2021-07-02;22(13):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  7. Early induction of functional SARS-CoV-2-specific T cells associates with rapid viral clearance and mild disease in COVID-19 patients
    Authors: AT Tan, M Linster, CW Tan, N Le Bert, WN Chia, K Kunasegara, Y Zhuang, CYL Tham, A Chia, GJD Smith, B Young, S Kalimuddin, JGH Low, D Lye, LF Wang, A Bertoletti
    Cell Reports, 2021-01-21;34(6):108728.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Serum microRNA181a: Correlates with the intracellular cytokine levels and a potential biomarker for acute graft-versus-host disease
    Authors: Linna Xie
    Cytokine, 2016-06-08;85(0):37-44.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Inherited IL-17RC deficiency in patients with chronic mucocutaneous candidiasis.
    Authors: Ling Y, Cypowyj S, Aytekin C, Galicchio M, Camcioglu Y, Nepesov S, Ikinciogullari A, Dogu F, Belkadi A, Levy R, Migaud M, Boisson B, Bolze A, Itan Y, Goudin N, Cottineau J, Picard C, Abel L, Bustamante J, Casanova J, Puel A
    J Exp Med, 2015-04-27;212(5):619-31.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  10. Comparative Multi-Donor Study of IFNgamma Secretion and Expression by Human PBMCs Using ELISPOT Side-by-Side with ELISA and Flow Cytometry Assays.
    Authors: Hagen J, Zimmerman R, Goetz C, Bonnevier J, Houchins J, Reagan K, Kalyuzhny A
    Cells, 2015-02-11;4(1):84-95.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. High-content cytometry and transcriptomic biomarker profiling of human B-cell activation.
    Authors: Hennig C, Ilginus C, Boztug K, Skokowa J, Marodi L, Szaflarska A, Sass M, Pignata C, Kilic S, Caragol I, Baumann U, Klein C, Welte K, Hansen G
    J Allergy Clin Immunol, 2013-09-05;133(1):172-80.e1-10.
    Species: Human
    Sample Types: Whole Cells
    Applications: Chip Cytometry
  12. Conditional ligands for Asian HLA variants facilitate the definition of CD8+ T-cell responses in acute and chronic viral diseases.
    Authors: Chang C, Tan A, Or M, Toh K, Lim P, Chia A, Froesig T, Nadua K, Oh H, Leong H, Hadrup S, Gehring A, Tan Y, Bertoletti A, Grotenbreg G
    Eur J Immunol, 2013-02-14;43(4):1109-20.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  13. NK cells from HCV-infected patients effectively induce apoptosis of activated primary human hepatic stellate cells in a TRAIL-, FasL- and NKG2D-dependent manner.
    Authors: Glassner A, Eisenhardt M, Kramer B, Korner C, Coenen M, Sauerbruch T, Spengler U, Nattermann J
    Lab. Invest., 2012-03-26;92(7):967-77.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. Cross-presentation of tumour antigens by human induced pluripotent stem cell-derived CD141(+)XCR1+ dendritic cells.
    Gene Ther, 2011-11-10;19(10):1035-40.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  15. Identification of human NK17/NK1 cells.
    Authors: Pandya AD, Al-Jaderi Z, Hoglund RA, Holmoy T, Harbo HF, Norgauer J, Maghazachi AA
    PLoS ONE, 2011-10-21;6(10):e26780.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  16. Human CD4(+) T cells recognize an epitope within alpha-fetoprotein sequence and develop into TGF-beta-producing CD4(+) T cells.
    Authors: Alisa A, Boswell S, Pathan AA, Ayaru L, Williams R, Behboudi S
    J. Immunol., 2008-04-01;180(7):5109-17.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  17. A reproducible method for the enumeration of functional (cytokine producing) versus non-functional peptide-specific cytotoxic T lymphocytes in human peripheral blood.
    Authors: Markovic SN, Nevala WK, Uhl CB, Celis E, McKean DJ
    Clin. Exp. Immunol., 2006-09-01;145(3):438-47.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  18. Plasma cell-like morphology of Th1-cytokine-producing cells associated with the loss of CD3 expression.
    Authors: Page G, Sattler A, Kersten S, Thiel A, Radbruch A, Miossec P
    Am. J. Pathol., 2004-02-01;164(2):409-17.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC-P
  19. Fatty Acid Synthase Contributes to Restimulation-Induced Cell Death of Human CD4 T Cells
    Authors: Kelsey Voss, Christopher R. Luthers, Katherine Pohida, Andrew L. Snow
    Frontiers in Molecular Biosciences

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