Human IFN-kappa Antibody

Catalog #: MAB102472 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB102472-100
MAB102472-SP

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All IFN-kappa Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IFN-kappa Antibody Summary

Species Reactivity

Human

Specificity

Detects human IFN‑ kappa in direct ELISAs.

Source

Monoclonal Mouse IgG_2A Clone # 1009727

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human IFN‑ kappa
Leu28-Lys207
Accession # Q90W0

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human IFN‑ kappa Monoclonal Antibody (Catalog # MAB10247).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA

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Human IFN‑ kappa ELISA Standard Curve. Recombinant Human IFN-kappa protein was serially diluted 2-fold and captured by Mouse Anti-Human IFN-kappa Monoclonal Antibody (Catalog # MAB10247) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human IFN-kappa Monoclonal Antibody (Catalog # MAB102472) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-kappa

Interferon kappa, also known as IFN-kappa, is a 207 aminoacids protein that in humans is encoded by the IFNK gene. IFN-kappa is a member of the type I interferon family which also includes IFN-alpha, -beta, -epsilon, and -omega. Type I interferons are a group of related glycoproteins that play an important role in host defenses against viral infections. Human IFN-kappa has been detected in keratinocytes, monocytes, and monocyte-derived dendritic cells and is reported to have contact-dependent antiviral activity. Human papillomavirus (HPV) 16 oncogene expression, which is necessary for the development of cervical cancer, has been shown to down-regulate human IFN-kappa expression.

Long Name

Interferon kappa

Entrez Gene IDs

56832 (Human); 387510 (Mouse)

Alternate Names

IFNK; IFNkappa; IFN-kappa