Human IGFBP-rp1/IGFBP-7 DuoSet ELISA Summary
* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
Product Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Kit Content
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Reagent Diluent*
Blocking Buffer*
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990), or equivalent
Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent
*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product
Scientific Data
Product Datasheets
Preparation and Storage
Background: IGFBP-rp1/IGFBP-7
The superfamily of insulin-like growth factor (IGF) binding proteins include the six high-affinity IGF binding proteins (IGFBP) and at least four additional low-affinity binding proteins referred to as IGFBP related proteins (IGFBP-rP). All IGFBP superfamily members are cysteine-rich proteins with conserved cysteine residues, which are clustered in the amino- and carboxy-terminal thirds of the molecule. IGFBPs modulate the biological activities of IGF proteins. Some IGFBPs may also have intrinsic bioactivity that is independent of their ability to bind IGF proteins. Post-translational modifications of IGFBP, including glycosylation, phosphorylation and proteolysis, have been shown to modify the affinities of the binding proteins to IGF. ALS (Acid Labile Subunit) is a liver-derived protein that exists in a ternary complex with Insulin-like Growth Factor (IGF)-binding Protein-3 (IGFBP-3) or IGFBP-5, and either IGF-I or IGF-II. ALS increases the half-life of IGF/IGFBP complexes in circulation.
Citations for Human IGFBP-rp1/IGFBP-7 DuoSet ELISA
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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Crosstalk between Cancer Cells and Cancer-Associated Fibroblasts Mediated by TGF-?1-IGFBP7 Signaling Promotes the Progression of Infiltrative Gastric Cancer
Authors: Hong, Z;Xie, W;Zhuo, H;Wei, X;Wang, K;Cheng, J;Lin, L;Hou, J;Chen, X;Cai, J;
Cancers
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Remote ischemic preconditioning causes transient cell-cycle arrest and renal protection by a NF-kB-dependent Sema5B pathway
Authors: J Rossaint, M Meersch, K Thomas, S Mersmann, M Lehmann, J Skupski, T Tekath, P Rosenberge, JA Kellum, H Pavenstädt, A Zarbock
JCI Insight, 2022-07-22;0(0):.
Species: Mouse
Sample Types: Serum
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Derivation and validation of urinary TIMP-1 for the prediction of acute kidney injury and mortality in critically ill children
Authors: H Huang, Q Lin, X Dai, J Chen, Z Bai, X Li, F Fang, Y Li
Journal of Translational Medicine, 2022-02-23;20(1):102.
Species: Human
Sample Types: Urine
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Combining renal cell arrest and damage biomarkers to predict progressive AKI in patient with sepsis
Authors: X Tao, C Chen, W Luo, J Zhou, J Tian, X Yang, FF Hou
Bmc Nephrology, 2021-12-15;22(1):415.
Species: Human
Sample Types: Urine
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Urine Biomarkers for the Assessment of Acute Kidney Injury in Neonates with Hypoxic Ischemic Encephalopathy Receiving Therapeutic Hypothermia
Authors: J Rumpel, BJ Spray, VY Chock, MJ Kirkley, CL Slagle, A Frymoyer, SH Cho, KM Gist, R Blaszak, B Poindexter, SE Courtney
The Journal of pediatrics, 2021-09-20;0(0):.
Species: Human
Sample Types: Urine
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Serum and urine FGF23 and IGFBP-7 for the prediction of acute kidney injury in critically ill children
Authors: Z Bai, F Fang, Z Xu, C Lu, X Wang, J Chen, J Pan, J Wang, Y Li
BMC Pediatr, 2018-06-15;18(1):192.
Species: Human
Sample Types: Serum
FAQs
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View all ELISA FAQsReviews for Human IGFBP-rp1/IGFBP-7 DuoSet ELISA
Average Rating: 4.7 (Based on 3 Reviews)
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Works very well for serum and plasma at higher dilutions.
Fantastic performance. Remember this uses reagent kit 3 not 2 (most of the duosets I use require to 2) which messed me up at first. Used for human urine @ 1:500 dilution.
I used this kit for quantifying IGFBP7 in human serum. It works very well and easy to use protocol. I have used almost 6X 5kit so far.
