Human IgG₁ Antibody

Detection of Human IgG₁by Western Blot. Western blot shows lysates of purified human IgG₁, purified human IgG₂(negative control), purified human IgG₃(negative control), and human purified human IgG₄(negative control). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human IgG₁Monoclonal Antibody (Catalog # MAB9894) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IgG₁at approximately 240 kDa (as indicated). Human IgG (Catalog # MAB1101) is shown as a loading control.This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

Human IgG₁ELISA Standard Curve. Recombinant Human IgG₁protein was serially diluted 2-fold and captured by Mouse Anti-Human IgG₁Monoclonal Antibody (Catalog # MAB9894) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rabbit Anti-Human IgG Monoclonal Antibody (Catalog # MAB11012) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).