Do the Human IgG2 antibodies, Catalog #s MAB9794 and MAB97941, bind to the hinge region of IgG2?

Name: Human IgG 2 Antibody
Brand: R&D Systems
SKU: MAB9794
Price: 429 USD

We have not mapped the epitope of these antibodes. We observe no cross-reactivity to other closely related IgG isoforms IgG1, IgG3 and IgG4 by non-reducing Western blot.

Human IgG₂ Antibody

Catalog #: MAB9794 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB9794-100
MAB9794-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IgG₂ Antibody Summary

Species Reactivity

Human

Specificity

Detects human IgG₂ in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 985028

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human IgG2 protein

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA capture antibody when paired with Rabbit Anti-Human IgG Monoclonal Antibody (Catalog # MAB11012).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA

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Human IgG₂ELISA Standard Curve. Recombinant Human IgG₂protein was serially diluted 2-fold and captured by Mouse Anti-Human IgG₂Monoclonal Antibody (Catalog # MAB9794) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rabbit Anti-Human IgG Monoclonal Antibody (Catalog # MAB11012) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IgG2

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G2

Alternate Names

Ig gamma-2 chain C region; IgG2; IGHG2; immunoglobulin heavy constant gamma 2 (G2m marker)

Product Datasheets

Product Datasheet

COA

SDS

FAQs

Do the Human IgG2 antibodies, Catalog #s MAB9794 and MAB97941, bind to the hinge region of IgG2?
- We have not mapped the epitope of these antibodes. We observe no cross-reactivity to other closely related IgG isoforms IgG1, IgG3 and IgG4 by non-reducing Western blot.

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