Does the Human IgG4 Antibody (Cat# MAB9895) cross-react with IgG1, IgG2 or IgG3?

Name: Human IgG 4 Antibody
Brand: R&D Systems
SKU: MAB9895
Price: 449 USD

This antibody was obtained by IgG4 immunization. It does not detect IgG1, IgG2 and IgG3 by western blot. Considering that the different isotype subclasses differ in the constant region (and hinges), it likely recognizes an epitope in the Fc region.

Human IgG₄ Antibody

Catalog #: MAB9895
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB9895-100
MAB9895-SP

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All IgG4 Antibodies

Product Details

Citations (1)

FAQs

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IgG₄ Antibody Summary

Species Reactivity

Human

Specificity

Detects human IgG₄ in direct ELISAs.

Source

Monoclonal Mouse IgG_2A Clone # 985547

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Purified IgG₄ from normal human plasma

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

ELISA

This antibody functions as an ELISA capture antibody when paired with Rabbit Anti-Human IgG Monoclonal Antibody (Catalog # MAB11012).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human IgG₄by Western Blot. Western blot shows lysates of purified human IgG₁(negative control), purified human IgG₂(negative control), purified human IgG₃(negative control), and human purified human IgG₄. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human IgG₄Monoclonal Antibody (Catalog # MAB9895) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IgG₁at approximately 240 kDa (as indicated). Human IgG (Catalog # MAB1101) is shown as a loading control.This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

ELISA

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Human IgG₄ELISA Standard Curve. Recombinant Human IgG₄protein was serially diluted 2-fold and captured by Mouse Anti-Human IgG₄Monoclonal Antibody (Catalog # MAB9895) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rabbit Anti-Human IgG Monoclonal Antibody (Catalog # MAB11012) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IgG4

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G4

Alternate Names

Ig gamma-4 chain C region; IgG4; immunoglobulin heavy constant gamma 4 (G4m marker); MGC117419

Product Datasheets

Product Datasheet

COA

SDS

Citation for Human IgG₄ Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Skin basal cell carcinomas assemble a pro-tumorigenic spatially organized and self-propagating Trem2+ myeloid niche
Authors: Haensel, D;Daniel, B;Gaddam, S;Pan, C;Fabo, T;Bjelajac, J;Jussila, AR;Gonzalez, F;Li, NY;Chen, Y;Hou, J;Patel, T;Aasi, S;Satpathy, AT;Oro, AE;
Nature communications
Species: Human
Sample Types: Organoid
Applications: Neutralization Control

FAQs

Does the Human IgG4 Antibody (Cat# MAB9895) cross-react with IgG1, IgG2 or IgG3?
- This antibody was obtained by IgG4 immunization. It does not detect IgG1, IgG2 and IgG3 by western blot. Considering that the different isotype subclasses differ in the constant region (and hinges), it likely recognizes an epitope in the Fc region.

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