Human IGSF4B/SynCAM3 Antibody

Catalog #: AF3678
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3678
AF3678-SP

IGSF4B/SynCAM3 in BG01V Human Embryonic Stem Cells.

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Human IGSF4B/SynCAM3 Antibody Summary

Species Reactivity

Human

Specificity

Detects human IGSF4B/SynCAM3 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross‑reactivity with recombinant human (rh) IGSF4C is observed and less than 2% cross-reactivity with rhIGSF4, rhIGSF4D, and rhIGSF8 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human IGSF4B/SynCAM3 isoform 1
Pro21-Tyr329
Accession # Q8N126

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human IGSF4B/SynCAM3 (Catalog # 3678-S4)

Flow Cytometry

2.5 µg/10⁶ cells

Human differentiated neuron progenitor cells

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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IGSF4B/SynCAM3 in BG01V Human Embryonic Stem Cells. IGSF4B/SynCAM3 was detected in immersion fixed BG01V human embryonic stem cells differentiated into neurons using Goat Anti-Human IGSF4B/SynCAM3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3678) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGSF4B/SynCAM3

IGSF4B (immunoglobulin superfamily member 4B), also called CADM3, TSLL1, Necl‑1 or SynCAM-3, is a neural tissue-specific member of the nectin-like family of immunoglobulin superfamily (1, 2). It is a 48‑50 kDa type I transmembrane (TM) glycoprotein that is concentrated at non-junctional contact sites of neuronal axons and glial processes (2, 3). In myelinated peripheral nerve fibers, IGSF4B is concentrated at nodes of Ranvier in contact sites of Schwann cells (2). The 398 amino acid (aa) human IGSF4B contains a 24 aa signal sequence, a 306 aa extracellular domain (ECD), a 21 aa transmembrane domain and a 47 aa cytoplasmic domain. The ECD contains an N-terminal V-type Ig-like domain that is responsible for Ca⁺⁺-independent homophilic and heterophilic interactions with Nectin-1, Nectin-3 or Necl‑2 (IGSF-4) in trans. It also contains two C2-type Ig-like domains that are responsible for Ca⁺⁺-independent homophilic dimerization in cis that is thought to precede trans interaction (2, 4, 5). The cytoplasmic domain binds members of the MAGUK guanylate kinase subfamily, such as Dlg3, Pals2 and CASK (2). These activities are thought to play roles in adhesion and architecture at the synapse (2). We have also shown that IGSF4B promotes in vitro outgrowth of cortical neurons. Of two known splice variants, one shows an insertion of 34 aa near the mature N-terminus and probably represents a 60 kDa form. A smaller variant shows deletions of aa 185‑231, and 281‑398, which includes the first C2-type Ig-like domain and the TM segment. The IGSF4B ECD is highly conserved, sharing 95‑96% aa identity between human mouse, rat, canine and bovine sequences. The ECD of IGSF4A, B, C and D proteins share 35‑50% aa identity.

References

Fukuhara, H. et al. (2001) Oncogene 20:5401.
Kakunaga, S. et al. (2005) J. Cell Sci. 118:1267.
Fukami, T. et al. (2003) Gene 323:11.
Shingai, T. et al. (2003) J. Biol. Chem. 278:35421
Dong, X. et al. (2006) J. Biol. Chem. 281:10610.

Long Name

Immunoglobulin Superfamily, Member 4B

Entrez Gene IDs

57863 (Human); 94332 (Mouse); 360882 (Rat)

Alternate Names

BIgR; Brain immunoglobulin receptor; CADM3; cell adhesion molecule 3; IGSF4B; IGSF4BSynCAM3; Immunoglobulin superfamily member 4B; member 4B; Necl-1; NECL1TSLC1-like protein 1; Nectin-like protein 1; Synaptic cell adhesion molecule 3; SynCAM3; TSLC1-like 1; TSLL1; TSLL1SYNCAM3

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Citation for Human IGSF4B/SynCAM3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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