Home / IL-1 RAcP/IL-1 R3 / Human IL-1 RAcP/IL-1 R3 Antibody

Human IL-1 RAcP/IL-1 R3 Antibody

Catalog #: MAB676
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB676
MAB676-SP
Detection of IL‑1 RAcP/IL‑1 R3 in Human PBMC by Flow Cytometry.
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Citations (3)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-1 RAcP/IL-1 R3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-1RAcP/IL-1 R3 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-1 RI, rhIL-1 RII, rhST2, rhIL-18 R alpha, IL-1 Rrp2, rhIL-18 RAP, rhSIGIRR, rhIL-1 RAPL1, rhIL-1 RAPL2, rhTLR1, rhTLR2, rhTLR3, rhTLR4, rhTLR5, rhTLR7, rhTLR8, rhTLR9, rhTLR10, rhMD-1, rhMD-2, or recombinant mouse RP105 is observed.
Source
Monoclonal Mouse IgG1 Clone # 89412
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf21-derived recombinant human IL-1RAcP/IL-1 R3
Ser21-Glu359 (predicted)
Accession # Q9NPH3
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of IL-1 RAcP/IL-1 R3 antibody in Human PBMC antibody by Flow Cytometry. View Larger

Detection of IL‑1 RAcP/IL‑1 R3 in Human PBMC by Flow Cytometry. Human peripheral blood monocytes were stained with Human IL-1 RAcP/IL-1 R3 Monoclonal Antibody (Catalog # MAB676, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-1 RAcP/IL-1 R3

IL-1 Receptor Accessory Protein (also IL-1 R3) is a ubiquitous 70-90 kDa member of the interleukin-1 receptor family of proteins (1-5). It serves as a non-ligand-binding accessory component of the receptors for IL-1 alpha, IL-1 beta, and IL-33 (6, 7). Together with IRAK4 and MyD88, it generates a functional signaling complex with IL-1 RI; by itself, it generates a non-signaling, but high-affinity binding complex with IL-1 RII (8). In addition, it interacts with ST2 on mast cells and Th2 T cells to create a functional IL-33 receptor complex (7). Mature human IL-1 RAcP is a type I transmembrane glycoprotein that is 550 amino acids in length. It contains a 347 amino acid (aa) extracellular region (aa 21-367), a 21 aa transmembrane segment, and a 182 aa cytoplasmic domain (9). The extracellular region shows three C2-type Ig-like domains, the most membrane proximal of which is suggested to be responsible for dimerization with IL-1 RI (10). There are three alternative splice forms reported for IL-1 RAcP. One is transmembrane and shows a 239 aa substitution for the C-terminal 122 amino acids (11). The other two are soluble; one shows a six aa substitution for aa 351-570, while a second shows a 45 aa substitution for aa 302-579 (12, 13). The soluble receptor isoforms appear to be inhibitory to IL-1 signaling. When present with soluble IL-1 RII, soluble IL-1 RAcP increases the IL-1 binding affinity of IL-1 RII more than 100-fold, thus neutralizing the effects of IL-1 (14). The human and mouse IL-1 RAcP precursors are 89% aa identical; within the extracellular region, they share 86% aa identity.

References
  1. Subramaniam, S. et al. (2004) Dev. Comp. Immunol. 28:415.
  2. Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229.
  3. Dunne, A. and L.A.J. O'Neill (2003) Sci STKE. Feb 25;2003(171):re3.
  4. Huang, J. et al. (1997) Proc. Natl. Acad. Sci. USA 94:12829.
  5. Greenfeder, S. A. et al. (1995) J. Biol. Chem. 270:13757.
  6. Brikos, C. et al. (2007) Mol. Cell. Proteomics 6:1551.
  7. Chackerian, A.A. et al. (2007) J. Immunol. 179:2551.
  8. Lang, D. et al. (1998) J. Immunol. 161:6871.
  9. SwissProt. Accession # Q9NPH3.
  10. Yoon, D-Y. and C.A. Dinarello (1998) J. Immunol. 160:3170.
  11. Lu, H-L. et al. (2008) Mol. Immunol. 45:1374.
  12. Jensen, L.E. et al. (2000) J. Immunol. 164:5277.
  13. Jensen, L.E. and A.S. Whitehead (2003) Cell. Signal. 15:793.
  14. Smith, D.E. et al. (2003) Immunity 18:87.
 
Long Name
Interleukin 1 Receptor Accessory Protein
Entrez Gene IDs
3556 (Human); 16180 (Mouse)
Alternate Names
C3orf13IL-1RAcPIL1R3Interleukin-1 receptor 3; FLJ37788; IL-1 R3; IL-1 RAcP; IL-1 receptor accessory protein; IL-1R3; IL-1R-3; IL1RAcP; IL-1RAcP; IL1RAP; interleukin 1 receptor accessory protein; interleukin-1 receptor accessory protein beta; interleukin-1 receptor accessory protein

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Citations for Human IL-1 RAcP/IL-1 R3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Proteomic Screens for Suppressors of Anoikis Identify IL1RAP as a Promising Surface Target in Ewing Sarcoma
    Authors: Zhang HF, Hughes CS, Li W et al.
    Cancer Discovery
  2. CML hematopoietic stem cells expressing IL-1RAP can be targeted by chimeric antigen receptor (CAR)-engineered T cells
    Authors: W Warda, F Larosa, M Neto Da Ro, R Trad, E Deconinck, Z Fajloun, C Faure, D Caillot, M Moldovan, S Valmary-De, S Biichle, E Daguindau, F Garnache-O, S Tabruyn, O Adotévi, M Deschamps, C Ferrand
    Cancer Res., 2018-12-04;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Proteomic Screens for Suppressors of Anoikis Identify IL1RAP as a Promising Surface Target in Ewing Sarcoma
    Authors: Zhang HF, Hughes CS, Li W et al.
    Cancer Discovery

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