Human IL-1 RII Antibody Summary
Phe14-Glu343 (Glu297Gly)
Accession # P27930
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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IL‑1 RII Inhibition of IL‑1 beta /IL‑1F2‑dependent Cell Proliferation and Neutralization by Human IL‑1 RII Antibody. Recombinant Human IL-1 RII (263-2R) inhibits Recombinant Human IL-1 beta / IL-1F2 (201-LB) induced proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IL-1 beta /IL-1F2 (50 pg/mL) activity elicited by Recombinant Human IL-1 RII (2 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-1 RII Monoclonal Antibody (Catalog # MAB263). The ND50 is typically 2-6 µg/mL.
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Detection of IL‑1 RII in HDLM-2 (positive) and K562 (negative). IL‑1 RII was detected in immersion fixed HDLM‑2 human Hodgkin’s lymphoma cells (positive) and absent in K562 human chronic myelogenous leukemia cells (negative) using Mouse Anti-Human IL‑1 RII Monoclonal Antibody (Catalog # MAB263) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surface. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
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Detection of IL‑1 RII in HDLM-2 cells by Flow Cytometry HDLM-2 cells were stained with Mouse Anti-Human IL‑1 RII Monoclonal Antibody (Catalog # MAB263, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-1 RII
Two distinct types of receptors that bind the pleiotropic cytokines IL-1 alpha and IL-1 beta have been described. The IL-1 receptor type I is an 80 kDa transmembrane protein that is expressed predominantly by T cells, fibroblasts and endothelial cells. IL-1 receptor type II is a 68 kDa transmembrane protein found on B lymphocytes, neutrophils, monocytes, large granular leukocytes and endothelial cells. Both receptors are members of the immunoglobulin superfamily and show approximately 28% sequence similarity in their extracellular domains. The two receptor types do not heterodimerize in a receptor complex.
An IL-1 receptor accessory protein that can heterodimerize with the type I receptor in the presence of IL-1 alpha or IL-1 beta but not IL-1ra, was identified (1). This type I receptor complex appears to mediate all the known IL-1 biological responses. The receptor type II has a short cytoplasmic domain and does not transduce IL-1 signals. In addition to the membrane-bound form of IL-1 RII, a naturally-occurring soluble form of IL-1 RII has been described. It has been suggested that the type II receptor, either as the membrane-bound or as the soluble form, serves as a decoy for IL-1 and inhibits IL-1 action by blocking the binding of IL-1 to the signaling type I receptor complex.
Product Datasheets
Citations for Human IL-1 RII Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Induction of the IL-1RII decoy receptor by NFAT/FOXP3 blocks IL-1 beta -dependent response of Th17 cells
Authors: Dong Hyun Kim, Hee Young Kim, Sunjung Cho, Su-Jin Yoo, Won-Ju Kim, Hye Ran Yeon et al.
eLife
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Isolation and characterization of human trophoblast side-population (SP) cells in primary villous cytotrophoblasts and HTR-8/SVneo cell line.
Authors: Takao T, Asanoma K, Kato K
PLoS ONE, 2011-07-07;6(7):e21990.
Species: Human
Sample Types: Cell Lysates, Whole Cells
Applications: Cell Selection, ICC, Western Blot -
Immunolocalization of interleukin-1 receptors in the sarcolemma and nuclei of skeletal muscle in patients with idiopathic inflammatory myopathies.
Authors: Grundtman C, Salomonsson S, Dorph C, Bruton J, Andersson U, Lundberg IE
Arthritis Rheum., 2007-02-01;56(2):674-87.
Species: Human
Sample Types: Whole Cells, Whole Tissue
Applications: ICC, IHC-Fr
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