Home / IL-10 / Human IL-10 Fluorescein-conjugated Antibody

Human IL-10 Fluorescein-conjugated Antibody

Catalog #: IC2172F
3 Citations Datasheet / COA / SDS
Newer Version Available: IC9210G
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Discontinued Product

IC2172F has been discontinued and is replaced by IC9210G.

Detection of IL‑10 in Human PBMCs by Flow Cytometry.
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Citations (3)
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Human IL-10 Fluorescein-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-10 in ELISAs. In sandwich immunoassays, less than 5% cross-reactivity with recombinant equine IL-10 is observed and less than 0.5% cross-reactivity with recombinant canine IL-10, recombinant mouse IL-10, recombinant rat IL-10, recombinant porcine IL-10, recombinant cotton rat IL-10, and recombinant feline IL-10 is observed.
Source
Monoclonal Mouse IgG1 Clone # 127107
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-10
Ser19-Asn178
Accession # P22301
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of IL-10 antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of IL‑10 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) either (A) untreated or (B) treated with PMA and Calcium Ionomycin were stained with Mouse Anti-Human IL-10 Fluorescein-conjugated Monoclonal Antibody (Catalog # IC2172F) and Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (Catalog # FAB100A). Quadrant markers were set based on control antibody staining (Catalog # IC002F). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-10

Interleukin-10 (IL-10) is a pleiotropic cytokine that can exert either immunostimulatory or immunosuppressive effects on a variety of cell types. It is produced predominantly by Th2 lymphocytes and other activated lymphoid cells.

Long Name
Interleukin 10
Entrez Gene IDs
3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 102133450 (Cynomolgus Monkey); 493683 (Feline); 100715618 (Guinea Pig); 2949786 (Viral)
Alternate Names
CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; GVHDS; IL10; IL-10; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF

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Citations for Human IL-10 Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Native cellulose nanofibrills induce immune tolerance in vitro by acting on dendritic cells
    Sci Rep, 2016-08-25;6(0):31618.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Targeting of macrophage galactose-type C-type lectin (MGL) induces DC signaling and activation.
    Authors: Napoletano C, Zizzari IG, Rughetti A, Rahimi H, Irimura T, Clausen H, Wandall HH, Belleudi F, Bellati F, Pierelli L, Frati L, Nuti M
    Eur. J. Immunol., 2012-04-01;42(4):936-45.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. IRX-2, a novel biologic, favors the expansion of T effector over T regulatory cells in a human tumor microenvironment model.
    Authors: Schilling B, Harasymczuk M, Schuler P, Egan JE, Whiteside TL
    J. Mol. Med., 2011-09-14;90(2):139-47.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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