Human IL-12 p70 Antibody Summary
Arg23-Ser219 of p35, Ile23-Ser328 of p40
Accession # P29459(p35) & P29460 (p40)
Applications
Human IL-12 p70 Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
View Larger
IL‑12 p70 in THP‑1 Human Cell Line. IL‑12 p70 was detected in immersion fixed THP‑1 human acute monocytic leukemia cell line treated with 5 ng/mL PMA for 48 hours and 100 ng/mL LPS and 3 µM monensin for 24 hours (positive staining) and THP‑1 human acute monocytic leukemia cell line (untreated, negative staining) using Mouse Anti-Human IL‑12 p70 Monoclonal Antibody (Catalog # MAB611R) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-12 p70
Interleukin 12, also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a pleiotrophic cytokine originally identified in the medium of activated human B lymphoblastoid cell lines. The p40 subunit of IL-12 has been shown to have extensive amino acid sequence homology to the extracellular domain of the human IL-6 receptor while the p35 subunit shows distant but significant sequence similarity to IL-6, G-CSF, and chicken MGF. These observations have led to the suggestion that IL-12 might have evolved from a cytokine/soluble receptor complex. Human and murine IL-12 share 70% and 60% amino acid sequence homology in their p40 and p35 subunits, respectively. IL-12 apparently shows species specificity with human IL-12 reportedly showing minimal activity in the murine system. IL-12 is produced by macrophages and B lymphocytes and has been shown to have multiple effects on T cells and natural killer (NK) cells. These effects include inducing production of IFN-gamma and TNF by resting and activated T and NK cells, synergizing with other IFN-gamma inducers at both the transcriptional and post-transcriptional levels. This interaction induces IFN-gamma gene expression, enhancing the cytotoxic activity of resting NK and T cells, inducing and synergizing with IL-2 in the generation of lymphokine-activated killer (LAK) cells, acting as a co-mitogen to stimulate proliferation of resting T cells, and inducing proliferation of activated T and NK cells. Current evidence indicates that IL-12, produced by macrophages in response to infectious agents, is a central mediator of the cell-mediated immune response by its actions on the development, proliferation, and activities of TH1 cells. In its role as the initiator of cell-mediated immunity, it has been suggested that IL-12 has therapeutic potential as a stimulator of cell-mediated immune responses to microbial pathogens, metastatic cancers, and viral infections such as AIDS.
Product Datasheets
Citation for Human IL-12 p70 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
-
Post-acute sequelae of SARS-CoV-2 cardiovascular symptoms are associated with trace-level cytokines that affect cardiomyocyte function
Authors: Sinclair, JE;Vedelago, C;Ryan, FJ;Carney, M;Redd, MA;Lynn, MA;Grubor-Bauk, B;Cao, Y;Henders, AK;Chew, KY;Gilroy, D;Greaves, K;Labzin, L;Ziser, L;Ronacher, K;Wallace, LM;Zhang, Y;Macauslane, K;Ellis, DJ;Rao, S;Burr, L;Bain, A;Karawita, A;Schulz, BL;Li, J;Lynn, DJ;Palpant, N;Wuethrich, A;Trau, M;Short, KR;
Nature microbiology
Species: Human
Sample Types: Plasma
Applications: Bioassay
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Human IL-12 p70 Antibody
There are currently no reviews for this product. Be the first to review Human IL-12 p70 Antibody and earn rewards!
Have you used Human IL-12 p70 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
