Human IL-12 R beta 1 Antibody Summary
Cys24-Glu540
Accession # P42701
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
View Larger
Detection of IL‑12 R beta 1 in Daudi cells by Flow Cytometry Daudi cells were stained with Mouse Anti-Human IL‑12 R beta 1 Monoclonal Antibody (Catalog # MAB839, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-12 R beta 1
Interleukin12 (IL-12) is a key mediator of cellular-immunity and induces the differentiation of Th1 cells from precursor T helper cells. The biological activities of IL-12 are mediated through the high-affinity receptor complex composed of two subunits designated IL-12 R beta 1 and IL-12 R beta 2. Individually, IL-12 R beta 1 and IL-12 R beta 2 bind IL‑12 with low affinity. Co-expression of both subunits confers high-affinity binding and is required for IL-12 activity. Both IL-12 receptor subunits are type I transmembrane proteins that share similarities with the gp130/G-CSF R subgroup in the cytokine receptor superfamily. IL-12 R beta 1 cDNA encodes a 662 amino acid (aa) protein with a putative 23 aa signal peptide that is cleaved to generate the mature protein with a 522 aa extracellular domain, a 25 aa transmembrane domain and a 92 aa cytoplasmic region. Expression of IL-12 R beta 1 is detected in activated T cells, NK cells and B cells. The expression of IL‑12 R beta 2 is more restricted and appears to be limited to Th2 cells.
Product Datasheets
Citations for Human IL-12 R beta 1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
2
Citations: Showing 1 - 2
Filter your results:
Filter by:
-
Blood CD4+CD45RO+CXCR5+ T cells are decreased but partially functional in signal transducer and activator of transcription 3 deficiency.
Authors: Mazerolles F, Picard C, Kracker S, Fischer A, Durandy A
J Allergy Clin Immunol, 2013-02-10;131(4):1146-56, 1156.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Human intestinal intraepithelial lymphocytes keep TNF alpha levels low by cell uptake and feedback inhibition of transcription.
Authors: Ebert EC, Mehta V
Cell. Immunol., 2006-08-17;241(1):7-13.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Human IL-12 R beta 1 Antibody
Average Rating: 4 (Based on 1 Review)
Have you used Human IL-12 R beta 1 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Filter by:
workign good with 1:5 dilution for the detection of human IL12 R beta in the serum or plasma samples.
