Human IL-12 R beta 2 Antibody

Catalog #: MAB19591 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB19591
MAB19591-SP

Detection of Human IL‑12 R beta 2 by Western Blot.

1 Image

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-12 R beta 2 Antibody Summary

Species Reactivity

Human

Specificity

Detects human IL-12 R beta 2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human IL‑12 R beta 1 or recombinant mouse IL-12 R beta 1 is observed.

Source

Monoclonal Mouse IgG_2A Clone # 622509

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human IL-12 R beta 2
Lys24-Cys622
Accession # Q99665

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Human IL‑12 R beta 2 by Western Blot. Western blot shows lysates of human CD4^{+in vitrodifferentiated Th1 cells. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human IL-12 R beta 2 Monoclonal Antibody (Catalog # MAB19591) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IL-12 R beta 2 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.}

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-12 R beta 2

IL-12 R beta 2 is a type I cytokine receptor family protein that associates with IL 12 R beta 1 to form a functional high affinity receptor complex for IL-12. The extracellular region of IL 12 R beta 2 contains five fibronectin type 3 domains. The cytoplasmic region is alternatively spliced, giving rise to two isoforms. IL-12 R beta 2 is up‑regulated in Th1 cells during inflammatory responses. The extracellular regions of human and mouse IL‑12 R beta 2 share 69% amino acid sequence identity.

Long Name

Interleukin 12 Receptor beta 2

Entrez Gene IDs

3595 (Human); 16162 (Mouse)

Alternate Names

IL-12 R beta 2; IL-12 receptor beta 2; IL-12 receptor subunit beta-2; IL12R beta 2; IL-12R subunit beta-2; IL12RB2; IL-12Rb2; IL-12R-beta-2; interleukin 12 receptor, beta 2; interleukin-12 receptor beta-2 chain; interleukin-12 receptor subunit beta-2