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Human IL-13 R alpha 1 Antibody

Catalog #: AF152
11 Citations 1 Review Datasheet / COA / SDS
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AF152
AF152-SP
Cell Proliferation Induced by IL‑13 and Neutralization by Human IL‑13 R alpha 1 Antibody.
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Product Details
Citations (11)
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Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-13 R alpha 1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL‑13 R alpha 1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinat human (rh) IL-13 R alpha 2, recombinant mouse IL-13 R alpha 1, rhIL-5 R alpha, rhIL-5 R beta, rhIL-4 R, and rhIL-9 R is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IL‑13 R alpha 1
Ala27-Thr343 (Thr130Ile)
Accession # Q5JSL4
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human IL‑13 R alpha 1 Fc Chimera (Catalog # 146-IR)
Flow Cytometry
2.5 µg/106 cells
See below
Immunohistochemistry
5-15 µg/mL
Immersion fixed paraffin-embedded sections of human skin and human heart.
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Neutralization
Measured by its ability to neutralize IL‑13-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 10-30 µg/mL in the presence of 10 ng/mL Recombinant Human IL‑13.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by IL‑13 and Neutralization by Human IL‑13 R alpha 1 Antibody. View Larger

Cell Proliferation Induced by IL‑13 and Neutralization by Human IL‑13 R alpha 1 Antibody. Recombinant Human IL-13 (Catalog # 213-ILB) stimulates prolif-eration in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-13 (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-13 Ra1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF152). The ND50 is typically 10-30 µg/mL.

Flow Cytometry Detection of IL‑13 Ra1 antibody in Human Granulocytes antibody by Flow Cytometry. View Larger

Detection of IL‑13 R alpha 1 in Human Granulocytes by Flow Cytometry. Human whole blood granulocytes were stained with Goat Anti-Human IL-13 Ra1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF152, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

Immunohistochemistry IL-13 Ra1 antibody in Human Skin by Immunohistochemistry (IHC-P). View Larger

IL‑13 R alpha 1 in Human Skin. IL-13 Ra1 was detected in immersion fixed paraffin-embedded sections of human skin using Goat Anti-Human IL-13 Ra1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF152) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry View Larger

IL‑13 R alpha 1 in Human Heart. IL‑13 R alpha 1 was detected in immersion fixed paraffin-embedded sections of Human Heart using Goat Anti-Human IL‑13 R alpha 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF152) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in myocardial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-13 R alpha 1

Two type 1 membrane proteins belonging to the hemopoietin receptor family have been cloned and shown to bind IL-13 with differing affinities. The lower affinity IL-13 binding protein, previously designated IL-13 R alpha, IL-13 R alpha ' or NR4, is now referred to as IL-13 R alpha 1. The high-affinity IL-13 binding protein, previously also designated IL-13 R or IL-13 R alpha ', is now referred to as IL-13 R alpha 2. The human IL-13 R alpha 1 was originally cloned based on sequence homology to the mouse IL-13 R alpha 1. The IL‑13 R alpha 1 cDNA encodes a 427 amino acid (aa) residue precursor protein with a putative 21 aa residue signal peptide, a 324 aa residue extracellular domain, a 23 aa residue transmembrane region and a 59 aa residue cytoplasmic tail. Human and mouse IL-13 R alpha 1 share 76% aa sequence identity. The extracellular domain of IL‑13 R alpha 1 is also closely related to that of IL-13 R alpha 2. IL-13 R alpha 1 has been shown to combine with the IL-4 R alpha to form a high-affinity receptor complex capable of transducing an IL-13-dependent proliferative signal. The role of IL-13 R alpha 2 in IL-13 signaling remains to be elucidated.

References
  1. Caput, D. et al. (1996) J. Biol. Chem. 271:16921.
  2. Donaldson, D.D. et al. (1998) J. Immunol. 161:2317.
  3. Aman, M.J. et al. (1996) J. Biol. Chem. 271:29265.
  4. Hilton, D.J. et al. (1996) Proc. Natl. Acad. Sci. USA 93:497.
  5. Zhang, J.G. et al. (1997) J. Biol. Chem. 272:9474.
Long Name
Interleukin 13 Receptor alpha 1
Entrez Gene IDs
3597 (Human); 16164 (Mouse)
Alternate Names
bB128O4.2.1 (interleukin 13 receptor, alpha 1); Cancer/testis antigen 19; CD213a1 antigen; CD213a1; CT19; IL-13 R alpha 1; IL13 receptor alpha-1 chain; IL-13 receptor subunit alpha-1; IL13R alpha 1; IL-13R subunit alpha-1; IL13R; IL13RA; IL-13Ra; IL13RA1; IL-13Ra1; IL-13R-alpha-1; interleukin 13 receptor, alpha 1; interleukin-13 receptor subunit alpha-1; NR4

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Citations for Human IL-13 R alpha 1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. Inclusion of 4-1BB Costimulation Enhances Selectivity and Functionality of IL13R?2-Targeted Chimeric Antigen Receptor T Cells
    Authors: Starr R, Aguilar B, Gumber D et al.
    Cancer Research Communications
  2. The IL-4/IL-13 signaling axis promotes prostatic fibrosis
    Authors: Q D'Arcy, M Gharaee-Ke, A Zhilin-Rot, JA Macoska
    PLoS ONE, 2022-10-06;17(10):e0275064.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells, Whole Tissue
    Applications: IHC, Neutralization, Western Blot
  3. Optimization of IL13R?2-Targeted Chimeric Antigen Receptor T Cells for Improved Anti-tumor Efficacy against Glioblastoma
    Authors: CE Brown, B Aguilar, R Starr, X Yang, WC Chang, L Weng, B Chang, A Sarkissian, A Brito, JF Sanchez, JR Ostberg, M D'Apuzzo, B Badie, ME Barish, SJ Forman
    Mol. Ther., 2017-10-05;26(1):31-44.
    Species: Human
    Sample Types: Xenograft
    Applications: Flow Cytometry
  4. Expression of surfactant protein D in airways of asthmatics and interleukin-13 modulation of surfactant protein D in human models of airway epithelium.
    Authors: Xu J, Singhera G, Dorscheid D
    Respir Res, 2015-02-15;16(0):26.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  5. IL-4 and IL-13 differentially regulate TLR-induced eosinophil-basophil differentiation of cord blood CD34+ progenitor cells.
    Authors: Reece P, Gauvreau G, Sehmi R, Denburg J
    PLoS ONE, 2014-06-27;9(6):e100734.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  6. Human osteoarthritic cartilage shows reduced in vivo expression of IL-4, a chondroprotective cytokine that differentially modulates IL-1beta-stimulated production of chemokines and matrix-degrading enzymes in vitro.
    Authors: Assirelli E, Pulsatelli L, Dolzani P, Platano D, Olivotto E, Filardo G, Trisolino G, Facchini A, Borzi R, Meliconi R
    PLoS ONE, 2014-05-12;9(5):e96925.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: Flow Cytometry, IHC-Fr
  7. The involvement of interleukin-1 and interleukin-4 in the response of human annulus fibrosus cells to cyclic tensile strain: an altered mechanotransduction pathway with degeneration
    Authors: Hamish T J Gilbert, Judith A Hoyland, Anthony J Freemont, Sarah J Millward-Sadler
    Arthritis Research & Therapy
  8. Obligatory role for interleukin-13 in obstructive lesion development in airway allografts.
    Authors: Lama VN, Harada H, Badri LN, Flint A, Hogaboam CM, McKenzie A, Martinez FJ, Toews GB, Moore BB, Pinsky DJ
    Am. J. Pathol., 2006-07-01;169(1):47-60.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  9. Classification of distinct subtypes of peripheral T-cell lymphoma unspecified, identified by chemokine and chemokine receptor expression: Analysis of prognosis.
    Authors: Ohshima K, Karube K, Kawano R, Tsuchiya T, Suefuji H, Yamaguchi T, Suzumiya J, Kikuchii M
    Int. J. Oncol., 2004-09-01;25(3):605-13.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  10. Differential effect of IL-4 and IL-13 on the expression of recombination-activating genes in mature B cells from human peripheral blood.
    Authors: Kajiwara K, Shinazawa M, Morishima H, Yanagihara Y
    Cell. Immunol., 2004-02-01;227(2):121-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Vascular endothelial growth factor ligands and receptors that regulate human cytotrophoblast survival are dysregulated in severe preeclampsia and hemolysis, elevated liver enzymes, and low platelets syndrome.
    Authors: Zhou Y, 2019, McMaster M, Woo K, Janatpour M, Perry J, Karpanen T, Alitalo K, Damsky C, Fisher SJ
    2002-04-01;160(4):1405-23.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P

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Human IL-13 R alpha 1 Antibody
By Kurt Shanebeck on 12/06/2017
Application: B/N Sample Tested: Recombinant protein Species: Human

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