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Human IL-15 Antibody

Catalog #: MAB247
21 Citations Datasheet / COA / SDS
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MAB247-SP
MAB247-100
MAB247-500
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Cell Proliferation Induced by IL‑15 and Neutralization by Human IL‑15 Antibody.
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Citations (21)
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Human IL-15 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-15 in Western blots. In Western blots, no cross-reactivity with recombinant human IL‑2 is observed.
Source
Monoclonal Mouse IgG1 Clone # 34593
Purification
Protein A or G purified from ascites
Immunogen
E. coli-derived recombinant human IL-15
Asn49-Ser162
Accession # P40933
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human IL‑15 (Catalog # 247-ILB)
Neutralization
Measured by its ability to neutralize IL‑15-induced proliferation in the MO7e human megakaryocytic leukemic cell line. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359. The Neutralization Dose (ND50) is typically 0.007-0.042 µg/mL in the presence of 10 ng/mL Recombinant Human IL‑15.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by IL‑15 and Neutralization by Human IL‑15 Antibody. View Larger

Cell Proliferation Induced by IL‑15 and Neutralization by Human IL‑15 Antibody. Recombinant Human IL-15 (Catalog # 247-ILB) stimulates proliferation in the MO7e human megakaryocytic leukemic cell line in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human IL-15 (10 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-15 Monoclonal Antibody (Catalog # MAB247). The ND50 is typically 0.007-0.042 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-15

Interleukin 15 (IL-15) is a widely expressed 14 kDa cytokine that is structurally and functionally related to IL-2 (1-3). Mature human IL-15 shares 70% amino acid sequence identity with mouse and rat IL-15. Alternate splicing generates isoforms of IL-15 with either a long or short signal peptide (LSP or SSP), and the SSP isoform is retained intracellularly (4). IL-15 binds with high affinity to IL-15 R alpha (5). It binds with lower affinity to a complex of IL-2 R beta and the common gamma chain ( gamma c) which are also subunits of the IL-2 receptor complex (1, 6). IL-15 associates with IL-15 R alpha in the endoplasmic reticulum, and this complex is expressed on the cell surface (7, 8). The dominant mechanism of IL-15 action is known as transpresentation in which IL-15 and IL-15 R alpha are coordinately expressed on the surface of one cell and interact with complexes of IL-2 R beta / gamma c on adjacent cells (9). This enables cells to respond to IL-15 even if they do not express IL-15 R alpha (8, 10). Soluble IL-15-binding forms of IL-15 R alpha can be generated by proteolytic shedding or alternate splicing (11-13). These molecules retain the ability to bind tightly to IL-15 and can either inhibit or augment IL-15 function (5, 12, 13). Consistent with its shared use of IL-2 receptor subunits, IL-15 induces IL-2-like effects in lymphocyte development and homeostasis (3). It is particularly important for the maintenance and activation of NK cells and CD8+ memory T cells (3). IL-15 also exerts pleiotropic effects on other hematopoietic cells and non-immune cells (2). Ligation of membrane-associated IL-15/IL-15 R alpha complexes induces reverse signaling that promotes cellular adhesion, tyrosine phosphorylation of intracellular proteins, and cytokine secretion by the IL-15/IL-15 R alpha expressing cells (14, 15).

References
  1. Grabstein, K. et al. (1994) Science 264:965.
  2. Budagian, V. et al. (2006) Cytokine Growth Factor Rev. 17:259.
  3. Ma, A. et al. (2006) Annu. Rev. Immunol. 24:657.
  4. Tagaya, Y. et al. (1997) Proc. Natl. Acad. Sci. USA 94:14444.
  5. Giri, J.G. et al. (1995) EMBO 14:3654.
  6. Giri, J. et al. (1994) EMBO J. 13:2822.
  7. Duitman, E.H. et al. (2008) Mol. Cell. Biol. 28:4851.
  8. Dubois, S. et al. (2002) Immunity 17:537.
  9. Stonier, S.W. and K.S. Schluns (2010) Immunol. Lett. 127:85.
  10. Burkett, P.R. et al. (2004) J. Exp. Med. 200:825.
  11. Budagian, V. et al. (2004) J. Biol. Chem. 279:40368.
  12. Mortier, E. et al. (2004) J. Immunol. 173:1681.
  13. Bulanova, E. et al. (2007) J. Biol. Chem. 282:13167.
  14. Budagian, V. et al. (2004) J. Biol. Chem. 279:42192.
  15. Neely, G.G. et al. (2004) J. Immunol. 172:4225.
Long Name
Interleukin 15
Entrez Gene IDs
3600 (Human); 16168 (Mouse); 25670 (Rat); 102119613 (Cynomolgus Monkey); 493682 (Feline)
Alternate Names
IL15; IL-15; IL-15MGC9721; interleukin 15; interleukin-15

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Citations for Human IL-15 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

21 Citations: Showing 1 - 10
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  1. Protective heterologous T cell immunity in COVID-19 induced by the trivalent MMR and Tdap vaccine antigens
    Authors: Vijayashree Mysore, Xavier Cullere, Matthew L. Settles, Xinge Ji, Michael W. Kattan, Michaël Desjardins et al.
    Med (N Y)
  2. Gut Bacteria Induce Granzyme B Expression in Human Colonic ILC3s In Vitro in an IL-15–Dependent Manner
    Authors: Moriah J. Castleman, Stephanie M. Dillon, Tezha A. Thompson, Mario L. Santiago, Martin D. McCarter, Edward Barker et al.
    The Journal of Immunology
  3. Transmembrane-Bound IL-15–Promoted Epithelial-Mesenchymal Transition in Renal Cancer Cells Requires the Src-Dependent Akt/GSK-3 beta / beta -Catenin Pathway12
    Authors: Huaqin Yuan, Xiaoxin Meng, Wenjie Guo, Peifen Cai, Wanshuai Li, Qian Li et al.
    Neoplasia
  4. Complement-activated interferon-gamma –primed human endothelium transpresents interleukin-15 to CD8+ T cells
    Authors: Catherine B. Xie, Bo Jiang, Lingfeng Qin, George Tellides, Nancy C. Kirkiles-Smith, Dan Jane-wit et al.
    Journal of Clinical Investigation
  5. IL-15R alpha membrane anchorage in either cis or trans is required for stabilization of IL-15 and optimal signaling
    Authors: Agnès Quéméner, Sébastien Morisseau, Rui P. Sousa, Kilian Trillet, Mike Maillasson, Isabelle Leray et al.
    Journal of Cell Science
  6. Clinical manufacturing of recombinant human interleukin 15. I. Production cell line development and protein expression in E. coli with stop codon optimization
    Authors: Vinay V. Vyas, Dominic Esposito, Terry L. Sumpter, Trevor L. Broadt, James Hartley, George C. Knapp IV et al.
    Biotechnology Progress
  7. Human Liver Memory CD8+ T Cells Use Autophagy for Tissue Residence
    Authors: L Swadling, LJ Pallett, MO Diniz, JM Baker, OE Amin, KA Stegmann, AR Burton, NM Schmidt, A Jeffery-Sm, N Zakeri, K Suveizdyte, F Froghi, G Fusai, WM Rosenberg, BR Davidson, A Schurich, AK Simon, MK Maini
    Cell Rep, 2020-01-21;30(3):687-698.e6.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Scalable, cGMP-compatible purification of extracellular vesicles carrying bioactive human heterodimeric IL-15/lactadherin complexes
    Authors: DC Watson, BC Yung, C Bergamasch, B Chowdhury, J Bear, D Stellas, A Morales-Ka, JC Jones, BK Felber, X Chen, GN Pavlakis
    J Extracell Vesicles, 2018-02-28;7(1):1442088.
    Species: Human
    Sample Types: Extracellular Vesicles
    Applications: Transmission Electron Microsco
  9. Targeting the binding interface on a shared receptor subunit of a cytokine family enables the inhibition of multiple member cytokines with selectable target spectrum.
    Authors: Nata T, Basheer A, Cocchi F, van Besien R, Massoud R, Jacobson S, Azimi N, Tagaya Y
    J Biol Chem, 2015-07-16;290(37):22338-51.
    Species: Human
    Sample Types: Protein
    Applications: Neutralization
  10. IL-15 Expression on RA Synovial Fibroblasts Promotes B Cell Survival.
    Authors: Benito-Miguel M, Garcia-Carmona Y, Balsa A, Bautista-Caro MB, Arroyo-Villa I, Cobo-Ibanez T, Bonilla-Hernan MG, De Ayala CP, Sanchez-Mateos P, Martin-Mola E, Miranda-Carus ME
    PLoS ONE, 2012-07-09;7(7):e40620.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  11. Development of an IL-15-autocrine CD8 T-cell leukemia in IL-15-transgenic mice requires the cis expression of IL-15R alpha.
    Authors: Sato N, Sabzevari H, Fu S, Ju W, Petrus MN, Bamford RN, Waldmann TA, Tagaya Y
    Blood, 2011-02-08;117(15):4032-40.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  12. TLR3 and Rig-like receptor on myeloid dendritic cells and Rig-like receptor on human NK cells are both mandatory for production of IFN-gamma in response to double-stranded RNA.
    Authors: Perrot I, Deauvieau F, Massacrier C
    J. Immunol., 2010-07-16;185(4):2080-8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  13. Differential survival of leukocyte subsets mediated by synovial, bone marrow, and skin fibroblasts: site-specific versus activation-dependent survival of T cells and neutrophils.
    Authors: Filer A, Parsonage G, Smith E, Osborne C, Thomas AM, Curnow SJ, Rainger GE, Raza K, Nash GB, Lord J, Salmon M, Buckley CD
    Arthritis Rheum., 2006-07-01;54(7):2096-108.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  14. Cloning and expression of feline interleukin 15.
    Authors: Dean GA, Barger A, Lavoy A
    Cytokine, 2005-01-21;29(2):77-83.
    Species: Feline
    Sample Types: Whole Cells
    Applications: ICC, Western Blot
  15. Distinct roles of IL-12 and IL-15 in human natural killer cell activation by dendritic cells from secondary lymphoid organs.
    Authors: Ferlazzo G, Pack M, Thomas D, Paludan C, Schmid D, Strowig T, Bougras G, Muller WA, Moretta L, Munz C
    Proc. Natl. Acad. Sci. U.S.A., 2004-11-09;101(47):16606-11.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  16. IL-15 and the initiation of cell contact-dependent synovial fibroblast-T lymphocyte cross-talk in rheumatoid arthritis: effect of methotrexate.
    Authors: Miranda-Carus ME, Balsa A, Benito-Miguel M, Perez de Ayala C, Martin-Mola E
    J. Immunol., 2004-07-15;173(2):1463-76.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  17. Fibroblasts from human spleen regulate NK cell differentiation from blood CD34(+) progenitors via cell surface IL-15.
    Authors: Briard D, Brouty-Boye D, Azzarone B, Jasmin C
    J. Immunol., 2002-05-01;168(9):4326-32.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  18. Regulatory activity of autocrine IL-10 on dendritic cell functions.
    Authors: Corinti S, la Sala A, Pastore S
    J. Immunol., 2001-04-01;166(7):4312-8.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  19. Clearance of senescent decidual cells by uterine natural killer cells in cycling human endometrium
    Authors: Paul J Brighton, Yojiro Maruyama, Katherine Fishwick, Pavle Vrljicak, Shreeya Tewary, Risa Fujihara et al.
    eLife
  20. IL-15 negatively regulates curdlan-induced IL-23 production by human monocyte-derived dendritic cells and subsequent Th17 response
    Authors: Ahmet Eken, Zehra Okus, Serife Erdem, Zehra Busra Azizoglu, Yesim Haliloglu, Ayten Bicer et al.
    Northern Clinics of Istanbul
  21. Identification of a potent anti-IL-15 antibody with opposing mechanisms of action in vitro and in vivo
    Authors: D K Finch, A Midha, C L Buchanan, D Cochrane, R I Craggs, S Cruwys et al.
    British Journal of Pharmacology

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