Human IL-15 APC-conjugated Antibody Summary
Asn49-Ser162
Accession # P40933
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of IL‑15 in LPS-treated Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes, resting (open histogram), or treated overnight with 1 µg/mL LPS (filled histogram), were stained with Mouse Anti-Human IL-15 APC-conjugated Monoclonal Antibody (Catalog # IC2471A) or isotype control antibody (Catalog # IC002A, data not shown). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-15
Interleukin 15 (IL-15) is a widely expressed 14 kDa cytokine that is structurally and functionally related to IL-2 (1‑3). Mature human IL‑15 shares 70% amino acid sequence identity with mouse and rat IL-15. Alternate splicing generates isoforms of IL-15 with either a long or short signal peptide (LSP or SSP), and the SSP isoform is retained intracellularly (4). IL-15 binds with high affinity to IL-15 R alpha (5). It binds with lower affinity to a complex of IL-2 R beta and the common gamma chain ( gamma c) which are also subunits of the IL-2 receptor complex (1, 6). IL-15 associates with IL-15 R alpha in the endoplasmic reticulum, and this complex is expressed on the cell surface (7, 8). The dominant mechanism of IL-15 action is known as transpresentation in which IL-15 and IL-15 R alpha are coordinately expressed on the surface of one cell and interact with complexes of IL-2 R beta / gamma c on adjacent cells (9). This enables cells to respond to IL-15 even if they do not express IL-15 R alpha (8, 10). Soluble IL-15-binding forms of IL-15 R alpha can be generated by proteolytic shedding or alternate splicing (11-13). These molecules retain the ability to bind tightly to IL-15 and can either inhibit or augment IL-15 function (5, 12, 13). Consistent with its shared use of IL-2 receptor subunits, IL-15 induces IL-2-like effects in lymphocyte development and homeostasis (3). It is particularly important for the maintenance and activation of NK cells and CD8+ memory T cells (3). IL-15 also exerts pleiotropic effects on other hematopoietic cells and non-immune cells (2). Ligation of membrane-associated IL-15/IL-15 R alpha complexes induces reverse signaling that promotes cellular adhesion, tyrosine phosphorylation of intracellular proteins, and cytokine secretion by the IL-15/IL-15 R alpha expressing cells (14, 15).
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- Dubois, S. et al. (2002) Immunity 17:537.
- Stonier, S.W. and K.S. Schluns (2010) Immunol. Lett. 127:85.
- Burkett, P.R. et al. (2004) J. Exp. Med. 200:825.
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Product Datasheets
Citations for Human IL-15 APC-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Complement-activated interferon-gamma –primed human endothelium transpresents interleukin-15 to CD8+ T cells
Authors: Catherine B. Xie, Bo Jiang, Lingfeng Qin, George Tellides, Nancy C. Kirkiles-Smith, Dan Jane-wit et al.
Journal of Clinical Investigation
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Inflamm-aging influences immune cell survival factors in human bone marrow
Eur. J. Immunol, 2017-01-11;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
FAQs
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What concentration of LPS was used to stimulate PBMCs prior to staining with Human IL-15 Antibody, Catalog #s MAB2471 and IC2471?
PBMCs were stimulated overnight with 1 μg/mL LPS prior to staining with Human IL-15 Antibody. 3mM Monesin was added for the last 3-4 hours of stimulation.
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