Human IL-17/IL-17A Alexa Fluor® 488-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: IL-17/IL-17A

Interleukin 17, also known as IL-17A and CTLA-8, is a 20-22 kDa, secreted pleiotropic cytokine that exhibits a high degree of homology to a protein encoded by the ORF13 gene of herpesvirus Saimiri. Human IL-17 cDNA encodes a 155 amino acid (aa) residue precursor protein with a 23 amino acid residue signal peptide that is cleaved to yield the 132 aa residue mature IL-17. Both recombinant and natural IL-17 have been shown to exist as disulfide linked homodimers. IL-17 is also known to form a heterodimer with 20-24 kDa IL-17F. At the amino acid level, hIL-17 shows 62% aa sequence identity with mouse IL-17. The receptor for the IL-17A homodimer and IL-17A:F heterodimer is reported to be a combination of IL-17 RA and IL-17 RC, with a possible contribution by IL-17 RD. The expression of IL-17 is widespread, and found associated with mast cells, LTi cells, B cells, gamma δ T cells, CD4⁺ Th17 cells, iNKT cells, neutrophils, intestinal Paneth cells, Type I ILCs and CD8⁺ T_c17 cells. IL-17 exhibits multiple biological activities on a variety of cells, including: the induction of IL-6 and IL-8 production by fibroblasts, the enhancement of surface expression of ICAM-1 on fibroblasts, activation of NF-kappa B and costimulation of T cell proliferation, the preservation of intestinal mucosal integrity via claudin synthesis, and the induction of antimicrobial peptides by epithelium.

Product Datasheets

Product Specific Notices

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

Related Research Areas

• B Cell Chemotaxis, Migration, and Adhesion
• Cytokines and Receptors in Tfh Cell Differentiation and Function
• gamma delta T Cells
• Helper T (Th) Cells
• IL-17 Family
• LTi Cells
• Macrophage Activation Markers
• Microglia Activation Markers
• Molecules Secreted by VSMC
• Multiple Sclerosis and Myelin Related Molecules
• Natural Killer T (NKT) Cells
• NCR-ILC3
• Neuroinflammatory Factors
• Search Cytokine and Growth Factor Products
• T Cell Cytokine Signaling
• Th17 Cells