Human IL-18 BPa Antibody

Catalog #: MAB1191
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB1191
MAB1191-SP

Neutralization of IL-18 BPa Bioactivity by Human IL-18 BPa Antibody

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Citations (2)

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Human IL-18 BPa Antibody Summary

Species Reactivity

Human

Specificity

Detects human IL-18 BPa in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse (rm) IL-18 BPc or rmIL-18 BPd is observed.

Source

Monoclonal Mouse IgG₁ Clone # 136007

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human IL-18 BPa
Thr29-Gly192
Accession # AAD17190

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Neutralization

Measured by its ability to neutralize IL-18 BPa inhibition of IL-18/IL-1F4-induced IFN-gamma secretion in the KG-1 human acute myelogenous leukemia cell line. The Neutralization Dose (ND₅₀) is typically 0.800-12.0 μg/mL in the presence of 1 μg/mL Recombinant Human IL-18 BPa Fc Chimera, 40 ng/mL Recombinant Human IL-18/IL-1F4, and 20 ng/mL Recombinant Human TNF-alpha.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Neutralization of IL-18 BPa Bioactivity by Human IL-18 BPa Antibody Mouse Anti-Human IL-18 BPa Monoclonal Antibody (Catalog # MAB1191) neutralizes Recombinant Human IL-18 BPa Fc Chimera (119-BP) inhibition of Recombinant Human IL-18/IL-1F4 (9124-IL) induced IFN gamma secretion in the KG-1 human acute myelogenous leukemia cell line as measured by the Human IFN-gamma Quantikine ELISA Kit (DIF50C). The ND₅₀ for this effect is typically 0.800-12.0 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-18 BPa

Interleukin 18 binding protein (IL-18 BP or IL-18 BPa) is a secreted glycoprotein, which functions as an IL-18 antagonist by binding to IL-18 and blocking its biological activity. IL‑18 BPa bears no amino acid sequence homology to the membrane-associated IL-18 and IL-1 receptor proteins. The gene for human IL-18 BPa has been localized to chromosome 11q13. It encodes for at least four isoforms by alternative splicing. The IL-18 BP isoforms a and c each contain one immunoglobulin (Ig)-like C2-type domain while isoforms b and d lack a complete Ig domain. The complete Ig domain has been shown to be essential to the binding and neutralizing properties of the binding proteins. Two isoforms of mouse IL18 BP (c and d) containing the complete Ig domain have also been isolated and shown to neutralize IL-18 bioactivity. Human and mouse IL-18 BPa share approximately 61% amino acid sequence identity. Several poxviruses also encode proteins with sequence similarity to the human and mouse IL-18 BPa. Viral IL-18 BPs have been shown to bind and inhibit IL-18 responses and may be involved in modulating host immune responses. The expression of IL-18 BP is markedly upregulated by IFN-gamma, suggesting that IL-18 activity is modulated by a negative feedback mechanism mediated by IL-18 BP.

References

Mühl, H. et al. (2000) Biochem. Biophys. Res. Commun. 267:960.
Kim, S-H. et al. (2000) Proc. Nat. Acad. Sci. USA 97:1190.
Calderara, S. et al. (2001) Virology 279:22.

Long Name

Interleukin 18 Binding Protein Isoform a

Entrez Gene IDs

10068 (Human); 84388 (Rat); 107126419 (Cynomolgus Monkey)

Alternate Names

IL18 BPa; IL-18 BPa; IL18BP; IL-18BP; IL18BPa; interleukin 18 binding protein; interleukin-18-binding protein; MC51L-53L-54L homolog gene product; tadekinig-alfa

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

IL-1 Family

Citations for Human IL-18 BPa Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Immunomodulatory IL-18 binding protein is produced by prostate cancer cells and its levels in urine and serum correlate with tumor status
Authors: Kazutoshi Fujita, Charles M. Ewing, William B. Isaacs, Christian P. Pavlovich
International Journal of Cancer
Toll-like receptor 8 agonist and bacteria trigger potent activation of innate immune cells in human liver.
Authors: Jo J, Tan A, Ussher J, Sandalova E, Tang X, Tan-Garcia A, To N, Hong M, Chia A, Gill U, Kennedy P, Tan K, Lee K, De Libero G, Gehring A, Willberg C, Klenerman P, Bertoletti A
PLoS Pathog, 2014-06-26;10(6):e1004210.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization