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Human IL-18 BPa Quantikine ELISA Kit

Catalog #: DBP180
12 Citations 1 Review Datasheet / COA / SDS
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DBP180
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QC27: Quantikine Immunoassay Control Set 860 for Human IL-18 BPa
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Human IL-18 BPa ELISA Calibrator Diluent RD5-18 Standard Curve
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Citations (12)
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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human IL-18 BPa Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Urine (10 uL)
Sensitivity
7.52 pg/mL
Assay Range
26.6 - 1,700 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Specificity
Natural and recombinant human IL-18 BPa
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.

Product Summary

The Quantikine Human IL-18 BPa Immunoassay is a 4.5 hour solid phase ELISA designed to measure IL-18 BPa in cell culture supernates, cell lysates, serum, and plasma. It contains NS0-expressed recombinant human IL-18 BPa and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate recombinant human IL-18 BPa. Results obtained using natural human IL-18 BPa showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-18 BPa.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of a known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of a known concentration were tested in separate assays to determine inter-assay precision. Assays were performed by at least three technicians using two lots of kit components

Cell Culture Supernates, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 123 404 789 136 434 841
Standard Deviation 5.5 9.49 21.1 9.47 32.8 59.1
CV% 4.5 2.3 2.7 7 7.6 7

Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 109 368 740 115 362 692
Standard Deviation 5.73 16.4 15.8 13.5 29.1 48.3
CV% 5.3 4.5 2.1 11.7 8 7

Recovery

The recovery of human IL-18 BPa spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=4) 106 98-114
EDTA Plasma (n=4) 106 93-113
Heparin Plasma (n=4) 105 92-119
Serum (n=4) 102 92-113

Linearity

To assess the linearity of the assay, samples containing high concentrations of human IL-18 BPa were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human IL-18 BPa ELISA Linearity

Scientific Data

N/A Human IL-18 BPa ELISA Calibrator Diluent RD5-18 Standard Curve View Larger

Human IL-18 BPa ELISA Calibrator Diluent RD5-18 Standard Curve

N/A Human IL-18 BPa ELISA Calibrator Diluent RD6-68 Standard Curve View Larger

Human IL-18 BPa ELISA Calibrator Diluent RD6-68 Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-18 BPa

Interleukin 18 binding proteins are secreted glycoproteins which antagonize IL-18 activity by binding IL-18 and blocking its biological activity. Alternative splicing of the IL-18 BP gene produces at least four isoforms that differ in affinity for IL-18. The IL-18 BP isoforms a and c each contain one immunoglobulin (Ig)-like C2-type domain while isoforms b and d lack a complete Ig-like domain.

Long Name:
Interleukin 18 Binding Protein Isoform a
Entrez Gene IDs:
10068 (Human); 84388 (Rat); 107126419 (Cynomolgus Monkey)
Alternate Names:
IL18 BPa; IL-18 BPa; IL18BP; IL-18BP; IL18BPa; interleukin 18 binding protein; interleukin-18-binding protein; MC51L-53L-54L homolog gene product; tadekinig-alfa
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 100 µL Assay Diluent
  3.   Add 100 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate and wash 4 times.
    8. 200 µL Substrate Solution
  8.   Add 200 µL of Conjugate to each well.
  9.   For Serum & Plasma Samples: Cover with a new plate sealer, and incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
    For Cell Culture Supernate/Urine Samples: Cover with a new plate sealer, and incubate at room temperature for 20 minutes. PROTECT FROM LIGHT.
    10. 50 µL Stop Solution
  10. Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human IL-18 BPa Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. Increased Levels of Caspase-1 and IL-1? Among Adults With Persistent Immune Activation After 12 Years of Suppressive Antiretroviral Therapy in the Infectious Diseases Institute HIV Treatment Cohort
    Authors: Nabatanzi, R;Ssekamatte, P;Castelnuovo, B;Kambugu, A;Nakanjako, D;
    Open forum infectious diseases
    Species: Human
    Sample Types: Plasma
  2. Role of NLRP3 inflammasome activation in HCC cell progression
    Authors: Dai, B;Cao, H;Hu, Y;Gong, Z;Huang, X;Chen, Y;Liu, F;Peng, X;Zhang, Y;Lei, X;
    Heliyon
    Species: Human
    Sample Types: Cell Culture Supernates
  3. Pharmacokinetic Study of rhIL-18BP and Its Effect on Radiation-Induced Cytokine Changes in Mouse Serum and Intestine
    Authors: W Cui, L Hull, A Zizzo, L Wang, B Lin, M Zhai, M Xiao
    Toxics, 2022-12-30;11(1):.
    Species: Mouse
    Sample Types: Serum
  4. Expression of Circulating MicroRNAs and Myokines and Interactions with Serum Osteopontin in Type 2 Diabetic Patients with Moderate and Poor Glycemic Control: A Biochemical and Molecular Study
    Authors: HA Al-Rawaf, AH Alghadir, SA Gabr
    BioMed Research International, 2021-12-07;2021(0):7453000.
    Species: Human
    Sample Types: Serum
  5. The Effects of Beverage Intake after Exhaustive Exercise on Organ Damage, Inflammation and Oxidative Stress in Healthy Males.
    Authors: Tominaga T, Ikemura T, Yada K, Kanda K, Sugama K, Ma S, Choi W, Araya M, Huang J, Nakamura N, Suzuki K
    Antioxidants (Basel), 2021-05-28;10(6):.
    Species: Human
    Sample Types: Urine
  6. A pilot study evaluating GSK1070806 inhibition of interleukin-18 in renal transplant delayed graft function
    Authors: E Wlodek, RB Kirkpatric, S Andrews, R Noble, R Schroyer, J Scott, CJE Watson, M Clatworthy, EM Harrison, SJ Wigmore, K Stevenson, D Kingsmore, NS Sheerin, O Bestard, HA Stirnadel-, L Abberley, M Busz, S DeWall, M Birchler, D Krull, KS Thorneloe, A Weber, L Devey
    PLoS ONE, 2021-03-08;16(3):e0247972.
    Species: Human
    Sample Types: Serum
  7. IL-18BP is a secreted immune checkpoint and barrier to IL-18 immunotherapy
    Authors: T Zhou, W Damsky, OE Weizman, MK McGeary, KP Hartmann, CE Rosen, S Fischer, R Jackson, RA Flavell, J Wang, MF Sanmamed, MW Bosenberg, AM Ring
    Nature, 2020-06-24;0(0):.
    Species: Human
    Sample Types: Plasma
  8. Quartz Dust Exposure Affects NLRP3 Inflammasome Activation and Plasma Levels of IL-18 and IL-1Ra in Iron Foundry Workers
    Authors: A Hedbrant, L Andersson, IL Bryngelsso, D Eklund, H Westberg, E Särndahl, A Persson
    Mediators Inflamm., 2020-01-07;2020(0):8490908.
    Species: Human
    Sample Types: Plasma
  9. Whole blood assay as a model for in vitro evaluation of inflammasome activation and subsequent caspase-mediated interleukin-1 beta release
    Authors: TAT Tran, HW Grievink, K Lipinska, C Kluft, J Burggraaf, M Moerland, D Tasev, KE Malone
    PLoS ONE, 2019-04-08;14(4):e0214999.
    Species: Human
    Sample Types: Cell Culture Supernates
  10. Evaluation of the Effect Derived from Silybin with Vitamin D and Vitamin E Administration on Clinical, Metabolic, Endothelial Dysfunction, Oxidative Stress Parameters, and Serological Worsening Markers in Nonalcoholic Fatty Liver Disease Patients
    Authors: A Federico, M Dallio, M Masarone, AG Gravina, R Di Sarno, C Tuccillo, V Cossiga, S Lama, P Stiuso, F Morisco, M Persico, C Loguercio
    Oxid Med Cell Longev, 2019;2019(0):8742075.
    Species: Human
    Sample Types: Serum
  11. Aberrant monocyte responses predict and characterize dengue virus infection in individuals with severe disease
    Authors: YK Yong, HY Tan, SH Jen, EM Shankar, SK Natkunam, J Sathar, R Manikam, SD Sekaran
    J Transl Med, 2017-05-31;15(1):121.
    Species: Human
    Sample Types: Plasma
  12. Human Mucosa-Associated Invariant T Cells Accumulate in Colon Adenocarcinomas but Produce Reduced Amounts of IFN-gamma.
    Authors: Sundstrom P, Ahlmanner F, Akeus P, Sundquist M, Alsen S, Yrlid U, Borjesson L, Sjoling A, Gustavsson B, Wong S, Quiding-Jarbrink M
    J Immunol, 2015-08-21;195(7):3472-81.
    Species: Human
    Sample Types: Cell Culture Supernates

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Human IL-18 BPa Quantikine ELISA Kit
By Milos Lazic on 05/08/2018
Sample Tested: Kupffer cells

Very good ELISA. Have been using it only in cell supernatants. Linear standard curve, easy to calculate IL-18 concentrations. Appropriate for concentration response curves. Used it instead of HTRF, due to higher sensitivity!

Human IL-18 BPa Quantikine ELISA Kit DBP180