Human IL-18/IL-1F4 Biotinylated Antibody

Catalog #: D045-6
11 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
D045-6

All IL-18/IL-1F4 Antibodies

Product Details

Citations (11)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-18/IL-1F4 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

This antibody reacts with human IL-18 on ELISA.

Source

Monoclonal Rat IgG_2A Clone # 159-12B

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human IL-18/IL-1F4

Formulation

25 μg IgG in 50 μL volume of PBS containing 1% BSA and 0.09% sodium azide

Label

Biotin

Applications

Recommended Concentration

Sample

Human IL-18/IL-1F4 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.5-2.0 µg/mL

Use in combination with:

Capture Reagent: Human IL‑18/IL‑1F4 Antibody (Catalog # D044-3)

Standard: Recombinant Human IL-18/IL-1F4 Protein (Catalog # B001-5)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

This antibody solution is stable for one year from the date of purchase when stored at 4°C.

Background: IL-18/IL-1F4

Interleukin 18 (IL-18) is a 18 kDa cytokine which identified as a costimulatory factor for production of interferon-gamma (IFN-gamma ) in response to toxic shock and shares functional similarities with IL-12. IL-18 is synthesized as a precursor 24 kDa molecule without a signal peptide and must be cleaved to produce an active molecule. IL-1 converting enzyme (ICE, Caspase-1) cleaves pro-IL-18 at aspartic acid in the P1 position, producing the mature, bioactive peptide that is readily released from the cells. It is reported that IL-18 is produced from Kupffer cells, activated macrophages, keratinocytes, intestinal epithelial cells, osteoblasts, adrenal cortex cells and murine diencephalon. IFN-gamma is produced by activated T or NK cells and plays critical roles in the defense against microbiral pathogens. IFN-gamma activates macrophages, enhances NK activity and B cell maturation, proliferation and Ig secretion, induces MHC class I and II antigens, and inhibits osteoclast activation. IL-18 acts on T helper type-1 (Th1) T cells and in combination with IL-12 strongly induces them to produce IFN-gamma. Pleiotropic effects of IL-18 has also been reported, such as, enhancement production of IFN-gamma and GM-CSF in peripheral blood mononuclear cells, production of Th1 cytokines, IL-2, GM-CSF and IFN-gamma in T cells, enhancement of Fas ligand expression by Th1 cells.

References

McCurnin, D., et al., Pediatrics 121, 945-956 (2008)
Haveman, L. M., et al., Int. Immunol. 18, 1521-1529 (2006)
Shigehara, K., et al., J. Immunol. 166, 642-649 (2001)
Dao, T., et al. Cell Immunol. 173, 230-235 (1996)
Micallef, M., et al. Eur. J. Immunol. 26, 1647-1651 (1996)
Ushio, S., et al. J. Immunol. 156, 4274-4279 (1996)
Okamura, H., et al. Nature 378, 88-91 (1995)

Long Name

Interleukin 18

Entrez Gene IDs

3606 (Human); 16173 (Mouse); 29197 (Rat); 397057 (Porcine); 574151 (Primate)

Alternate Names

Iboctadekin; IFN-gamma-inducing factor; IGIF; IGIFIL-1 gamma; IL18; IL-18; IL-18MGC12320; IL-1F4; IL1F4iboctadekin; IL-1g; Interferon gamma-inducing factor; interleukin 18 (interferon-gamma-inducing factor); Interleukin-1 gamma; interleukin-18

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human IL-18/IL-1F4 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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Unleashing Natural IL18 Activity Using an Anti-IL18BP Blocker Induces Potent Immune Stimulation and Antitumor Effects
Authors: Menachem, A;Alteber, Z;Cojocaru, G;Fridman Kfir, T;Blat, D;Leiderman, O;Galperin, M;Sever, L;Cohen, N;Cohen, K;Granit, RZ;Vols, S;Frenkel, M;Soffer, L;Meyer, K;Menachem, K;Galon Tilleman, H;Morein, D;Borukhov, I;Toporik, A;Perpinial Shahor, M;Tatirovsky, E;Mizrachi, A;Levy-Barda, A;Sadot, E;Strenov, Y;Eitan, R;Jakobson-Setton, A;Yanichkin, N;Ferre, P;Ophir, E;
Cancer immunology research
Species: Human
Sample Types: Serum
Applications: ELISA Detection
cAbl Kinase Regulates Inflammasome Activation and Pyroptosis via ASC Phosphorylation
Authors: Mikhail A. Gavrilin, Evan R. Prather, Alex D. Vompe, Christian C. McAndrew, Mark D. Wewers
The Journal of Immunology
Human M2 Macrophages Limit NK Cell Effector Functions through Secretion of TGF-? and Engagement of CD85j
Authors: SY Nuñez, A Ziblat, F Secchiari, NI Torres, JM Sierra, XL Raffo Irao, RE Araya, CI Domaica, MB Fuertes, NW Zwirner
J. Immunol., 2017-12-27;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development (Detection)
IL-18 associated with lung lymphoid aggregates drives IFN? production in severe COPD
Authors: E Briend, GJ Ferguson, M Mori, G Damera, K Stephenson, NA Karp, S Sethi, CK Ward, MA Sleeman, JS Erjefält, DK Finch
Respir. Res., 2017-08-22;18(1):159.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development (Detection)
Production and regulation of interleukin-1 family cytokines at the materno-fetal interface
Authors: LM Scott, AH Bryant, A Rees, B Down, RH Jones, CA Thornton
Cytokine, 2017-07-13;0(0):.
Species: Human
Sample Types: Tissue Culture Supernates
Applications: ELISA Development (Detection)
Regulatory Dendritic Cells Restrain NK Cell IFN-gamma Production through Mechanisms Involving NKp46, IL-10, and MHC Class I-Specific Inhibitory Receptors.
Authors: Spallanzani R, Torres N, Avila D, Ziblat A, Iraolagoitia X, Rossi L, Domaica C, Fuertes M, Rabinovich G, Zwirner N
J Immunol, 2015-07-31;195(5):2141-8.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development (Detection)
Leptin enhances the secretion of interleukin (IL)-18, but not IL-1beta, from human monocytes via activation of caspase-1.
Authors: Jitprasertwong P, Jaedicke K, Nile C, Preshaw P, Taylor J
Cytokine, 2013-11-23;65(2):222-30.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development (Capture)
Long-term weight loss decreases the nontraditional cardiovascular risk factors interleukin-18 and matrix metalloproteinase-9 in obese subjects.
Authors: Madsen EL, Bruun JM, Skogstrand K, Hougaard DM, Christiansen T, Richelsen B
Metab. Clin. Exp., 2009-07-01;58(7):946-53.
Species: Human
Sample Types: Serum
Applications: Luminex Development
Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine.
Authors: Hu H, Kwun J, Aizenstein BD, Knechtle SJ
Transplantation, 2009-06-27;87(12):1814-20.
Species: Human
Sample Types: Urine
Applications: Antibody Array Development
Development and validation of sandwich ELISA microarrays with minimal assay interference.
Authors: Gonzalez RM, Seurynck-Servoss SL, Crowley SA
J. Proteome Res., 2008-04-19;7(6):2406-14.
Species: Human
Sample Types: Serum
Applications: ELISA Microarray Development

Effect of serum content and diluent selection on assay sensitivity and signal intensity in multiplex bead-based immunoassays.
Authors: Pfleger C, Schloot N, ter Veld F
J. Immunol. Methods, 2007-10-22;329(1):214-8.
Species: Human
Sample Types: Serum
Applications: Luminex Development