Human IL-1ra/IL-1F3 Biotinylated Antibody

Catalog #: BAF280
8 Citations Datasheet / COA / SDS

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BAF280

Product Details

Citations (8)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-1ra/IL-1F3 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human IL-1ra/IL-1F3 in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant mouse IL‑1ra, recombinant human (rh) IL-1 alpha, rhIL-1 beta, rhIL-1 RI, and rhIL-1 RII is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human IL‑1ra/IL-1F3

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human IL‑1ra/IL‑1F3 (Catalog # 280-RA)

Human IL-1ra/IL-1F3 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.1-0.4 µg/mL

Use in combination with:

Capture Reagent: Human IL‑1ra/IL‑1F3 Antibody (Catalog # MAB280)

Standard: Recombinant Human IL-1ra/IL-1F3 Protein (Catalog # 280-RA)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-1ra/IL-1F3

IL-1ra was originally isolated from the urine of patients with monocytic leukemia and has also been purified from adherent monocytes. The naturally occurring, fully glycosylated form has an apparent molecular weight of about 25,000 Daltons. The protein shows 26% amino acid homology to IL-1 beta and 19% homology to IL-1 alpha. It will compete with either factor for receptor binding, but does not interact with either one. Human IL-1ra will bind to both types of IL-1 receptor (I and II) on human cells, but reportedly will not block binding to the type II receptor on murine pre-B cell lines. The recombinant, non-glycosylated form of IL-1ra blocks binding of IL-1 to its receptor equally as well as the naturally-occurring, glycosylated form. The IL-1ra has been shown to block the inflammatory responses induced by IL-1 both in vitro and in vivo. Currently, pre-clinical and clinical studies are underway to test possible therapeutic applications for IL-1ra in the treatment of sepsis, rheumatoid arthritis and chronic myelogenous leukemia.

Long Name

Interleukin 1 Receptor Antagonist

Entrez Gene IDs

3557 (Human); 16181 (Mouse); 60582 (Rat); 397499 (Porcine)

Alternate Names

DIRA; ICIL-1ra; IL1F3; IL-1F3; IL1ra; IL-1ra; IL-1ra3; IL1RN; IL-1RN; interleukin 1 receptor antagonist; IRAP; MVCD4

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human IL-1ra/IL-1F3 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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A novel hybrid promoter responsive to pathophysiological and pharmacological regulation
Authors: Maria C. Subang, Rewas Fatah, Carly Bright, Patricia Blanco, Mariana Berenstein, Ying Wu et al.
Journal of Molecular Medicine
Extending the dynamic range of biomarker quantification through molecular equalization
Authors: Newman, S;Wilson, B;Mamerow, D;Wollant, B;Nyein, H;Rosenberg-Hasson, Y;Maecker, H;Eisenstein, M;Soh, H;
bioRxiv
Species: Human
Sample Types: Serum
Applications: Bioassay
Mycobacterium tuberculosis Lipoarabinomannan Activates Human Neutrophils via a TLR2/1 Mechanism Distinct from Pam3CSK4
Authors: JS Hook, M Cao, K Weng, N Kinnare, JG Moreland
J. Immunol., 2019-12-23;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
Suppression of TNF-alpha and IL-1 signaling identifies a mechanism of homeostatic regulation of macrophages by IL-27.
Authors: Kalliolias GD, Gordon RA, Ivashkiv LB
J. Immunol., 2010-10-22;185(11):7047-56.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development
trans fatty acids and systemic inflammation in heart failure.
Authors: Mozaffarian D, Rimm EB, King IB, Lawler RL, McDonald GB, Levy WC
Am. J. Clin. Nutr., 2004-12-01;80(6):1521-5.
Species: Human
Sample Types: Plasma
Applications: ELISA Development
Direct comparison of traditional ELISAs and membrane protein arrays for detection and quantification of human cytokines.
Authors: Copeland S, Siddiqui J, Remick D
J. Immunol. Methods, 2004-01-01;284(1):99-106.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Array Development
Interleukin-12 induces sustained activation of multiple host inflammatory mediator systems in chimpanzees.
Authors: Lauw FN, Dekkers PE, te Velde AA, Speelman P, Levi M, Kurimoto M, Hack CE, Van Deventer SJ, van der Poll T
J. Infect. Dis., 1999-03-01;179(3):646-52.
Species: Primate - Pan troglodytes (Chimpanzee)
Sample Types: Plasma
Applications: ELISA Development
Transport study of interleukin-1 inhibitors using a human in vitro model of the blood-brain barrier
Authors: Elisabet O. Sjöström, Maxime Culot, Lisa Leickt, Mikael Åstrand, Erik Nordling, Fabien Gosselet et al.
Brain, Behavior, & Immunity - Health