Human IL-1Rrp2/IL-1R6 Antibody

Catalog #: MAB8721 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB8721-100
MAB8721-SP

CXCL8/Il-8 Secretion Induced by IL‑36 gamma /IL‑1F9 and Neutralization by Human IL‑1 Rrp2/IL‑1 R6 Antibody.

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Human IL-1Rrp2/IL-1R6 Antibody Summary

Species Reactivity

Human

Specificity

Detects human IL‑1 Rrp2/IL‑1 R6 in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 116004

Purification

Protein A or G purified from ascites

Immunogen

Mouse myeloma cell line NS0-derived recombinant human IL‑1 Rrp2/IL‑1 R6
Asp20-Tyr337
Accession # Q9HB29

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Neutralization

Measured by its ability to neutralize IL‑36 gamma /IL‑1F9-induced IL-8 secretion in the A431 human epithelial carcinoma cell line. The Neutralization Dose (ND₅₀) is typically 0.25-1.5 µg/mL in the presence of 30 ng/mL Recombinant Human IL‑36 gamma /IL‑1F9 (aa 18-169).

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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CXCL8/Il-8 Secretion Induced by IL‑36 gamma /IL‑1F9 and Neutralization by Human IL‑1 Rrp2/IL‑1 R6 Antibody. Recombinant Human IL-36 gamma/IL-1F9 (aa 18-169) (Catalog # 6835-IL) stimulates CXCL8/IL-8 secretion in the A431 human epithelial carcinoma cell line in a dose-dependent manner (orange line), as measured by the Human CXCL8/IL-8 Quantikine ELISA Kit (Catalog # D8000C). Il-8 Secretion elicited by Recombinant Human IL-36 gamma/IL-1F9 (aa 18-169) (30 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-1 Rrp2/IL-1 R6 Monoclonal Antibody (Catalog # MAB8721). The ND₅₀ is typically 0.25-1.5 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-1Rrp2/IL-1R6

The Interleukin 1 receptor family (IL-1 R) comprises at least eleven members including IL-1 RI (IL-1 R1), IL-1 RII (IL-1 R2), IL-1 RAcP (IL‑1 R3), ST2 (T1/IL‑1 R4), IL‑18 Ra (IL-1 Rrp/IL-1 R5), IL-1 Rrp2 (IL-1 RL2/IL-1 R6), IL-18 Rb (AcPL/IL-1 R7), IL-1RAPL‑1 (TIGIRR‑2/IL‑1 R8), and TIGIRR-1 (IL-1 R9) (1). All family members possess three immunoglobulin (Ig)-like domains in their extracellular region. Most members also have an intracellular TIR (Toll-like receptor/IL-1 receptor signaling) domain that is also conserved in the Toll-like receptor family. Related proteins, SIGIRR (single Ig domain-containing IL-1 R-related molecule) and IL-18BP, differ from the other members by having only one Ig domain (1). Human IL-1 Rrp2 cDNA encodes a 561 amino acid (aa) residue precursor protein with a putative 19 aa signal peptide and a 318 aa extracellular domain. It shares 67% and 65% amino acid sequence identity with rat and mouse IL 1 Rrp2, respectively. IL-1 Rrp2 is expressed in lung epitheium, brain vasculature, kidney, testis, onocytes, skin-derived keratinocytes, fibroblasts and, to a lesser extent, endothelial cells (2, 3). IL-1 Rrp2 has been shown to mediate the activation of the transcription factor NF kappa B by the IL-1 family ligands IL-1 F6, F8 or F9 (also known as IL-1 epsilon ), with IL-1RAcP as a co‑factor (3, 4). Response to IL-1 F9 is specifically antagonized by IL‑1 F5 (also known as IL-1δ), an IL-1 family ligand that is most closely related to IL-1ra (3). IL-1 Rrp2, IL‑1 F5, and IL-1 F9 are all up-regulated in lesional psoriasis skin, suggesting that the IL-1 Rrp2 mediated signaling pathway may take part in local inflammatory responses (3).

References

Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229.
Lovenberg, T. W. et al. (1996) J. Neuroimmunol. 70:113.
Debets, R. et al. (2001) J. Immunol. 167:1440.
Towne, J. E. et al. (2004) J. Biol. Chem. 279:13677.

Long Name

Interleukin 1 Receptor-like 2

Entrez Gene IDs

8808 (Human); 107527 (Mouse); 171106 (Rat)

Alternate Names

IL-1 R6; IL-1 Rrp2; IL1RL2; IL-1RL2; IL1Rrp2; IL-1Rrp2; IL1RRP2IL1R-rp2Interleukin-1 receptor-related protein 2; interleukin 1 receptor-like 2; interleukin-1 receptor-like 2