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Human IL-22 Quantikine ELISA Kit

R&D Systems | Catalog # D2200

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Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (100 µL), Serum (100 µL), EDTA Plasma (100 µL), Heparin Plasma (100 µL), Urine (100 µL)

Sensitivity

5.8 pg/mL

Assay Range

15.6-1000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
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Product Summary for Human IL-22 Quantikine ELISA Kit

The Quantikine Human IL-22 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human IL-22 in cell culture supernates, serum, plasma, and urine. It contains E. coli-expressed recombinant human IL-22 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IL-22 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring IL-22.

Product Specifications

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Species

Human

Specificity

Natural and recombinant human IL-22

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

Interference observed with 1 or more available related molecules.

精度

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 56.5 227 468 63.0 243 491
Standard Deviation 2.55 7.00 10.7 5.32 12.1 19.6
CV% 4.5 3.1 2.3 8.4 5.0 4.0

Recovery for Human IL-22 Quantikine ELISA Kit

The recovery of human IL-22 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=4) 107 104-110
EDTA Plasma (n=4) 97 90-103
Heparin Plasma (n=4) 95 86-104
Serum (n=4) 92 87-99

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of IL-22 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Human IL-22 ELISA Linearity

Scientific Data Images for Human IL-22 Quantikine ELISA Kit

Human IL-22 ELISA Standard Curve

Human IL-22 ELISA Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-22

IL-22 (Interleukin-22) is a cytokine that induces the production of reactive oxygen species, IL-6, IL-10, and TNF-alpha as well as neutrophil infiltration during inflammation. It also supports the integrity of epithelial barriers and induces epithelial cell proliferation during wound healing. IL-22 signals through a receptor complex consisting of IL-22 R and IL-10 R beta. IL-10 R beta is a shared component of the receptor complexes for IL-10, IL-26, IL-28, and IL-29. IL-22 additionally binds to IL-22BP which blocks the interaction of IL-22 with IL-22 R.

Long Name

Interleukin 22

Alternate Names

IL-TIF, IL22

Entrez Gene IDs

50616 (Human); 50929 (Mouse); 500836 (Rat)

Gene Symbol

IL22

Additional IL-22 Products

Product Documents for Human IL-22 Quantikine ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human IL-22 Quantikine ELISA Kit

For research use only

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human IL-22 Quantikine ELISA Kit

Customer Reviews for Human IL-22 Quantikine ELISA Kit (4)

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  • Human IL-22 Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Heparin Plasma
    Verified Customer | Posted 01/11/2021
  • Human IL-22 Quantikine ELISA Kit
    Name: Gerre Lafond
    Sample Tested: Bowes human melanoma cell line
    Verified Customer | Posted 05/17/2018
  • Human IL-22 Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: B16-F10 mouse melanoma cell line
    Verified Customer | Posted 11/30/2017
  • Human IL-22 Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Serum
    Verified Customer | Posted 09/07/2017
    The IL-22 Quantikine ELISA performed as expected with low % CV's, and reproducible results.
    Human IL-22 Quantikine ELISA Kit D2200

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Protocols

View specific protocols for Human IL-22 Quantikine ELISA Kit (D2200):

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 100 µL Assay Diluent
  3.   Add 100 µL of Assay Diluent to each well.
    4. 100 µL Standard, Control, or Sample
  4.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate and wash 4 times.
    8. 200 µL Substrate Solution
  8.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
    9. 50 µL Stop Solution
  9.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

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