Human IL-22 R alpha 1 Antibody Summary
Pro18-Thr228
Accession # Q8N6P7
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of IL‑22 R alpha 1 in COLO 205 Human Cell Line by Flow Cytometry. COLO 205 human colorectal adenocarcinoma cell line was stained with Mouse Anti-Human IL-22 Ra1 Monoclonal Antibody (Catalog # MAB2770, filled histogram) or isotype control antibody (MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (F0102B).
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IL‑22 R alpha 1 in HT‑29 Human Cell Line. IL-22 Ra1 was detected in immersion fixed HT-29 human colon adenocarcinoma cell line using Mouse Anti-Human IL-22 Ra1 Monoclonal Antibody (Catalog # MAB2770) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Detection of IL‑22 R alpha 1 in HT‑29 Human Colon Adenocarcinoma Cell Line (Positive) and HepG2 Human Hepatocellular Carcinoma Cell Line (Negative) Cells. IL‑22 R alpha 1 was detected in immersion fixed HT‑29 human colon adenocarcinoma cell line (positive) and HepG2 human hepatocellular carcinoma cell line (negative) cells using Mouse Anti-Human IL‑22 R alpha 1 Monoclonal Antibody (Catalog # MAB2770) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surface and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Detection of IL‑22 R alpha 1 in HT-29 cells by Flow Cytometry. HT-29 cells were stained with Mouse Anti-Human IL‑22 R alpha 1 Monoclonal Antibody (Catalog # MAB2770, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
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Detection of Human IL-22R alpha 1 by Western Blot miR-197 suppresses the expression of IL22RA1 by binding to its 3′UTR.a) miR-197 binding site on IL22RA1 3′UTR. b) HEK-293T cells were transfected with psi-CHECK2 vectors encoding luciferase (vector), luciferase fused to the IL22RA1-3′UTR (IL22RA1 wt 3′UTR), or luciferase fused to a mutated in miR-197 binding to the IL22RA1 3′UTR (IL22RA1 mut 3′UTR) together with 2 µg miR-197 expressing plasmid or 2 µg scramble RNA expressing plasmid. Cells transfected with only with vector lacking the IL22RA1 3′UTR was valued as 100%. The error bars are calculated as standard error of at least 6 independent experiments. c) Western blot (WB) analysis of IL22RA1 protein 48 h after transfection with 5/10 nM of scramble control pre-miR or with 5/10 nM of pre-miR-197. d) Densitometry analysis of 4 WBs analysis of IL22RA1 protein 48 h after transfection with 10 nM of scramble control RNA (scramb), or 10 nM pre-miR-197 *p = 0.0000818. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25208211), licensed under a CC-BY license. Not internally tested by R&D Systems.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-22 R alpha 1
IL-22 receptor, also known as IL-22 R alpha 1 and CRF2-9, is an approximately 65 kDa transmembrane glycoprotein in the type II cytokine receptor family (CRF). IL‑22 R alpha 1 contains a 211 amino acid (aa) extracellular domain (ECD) with two fibronectin type III repeats, and a 323 aa cytoplasmic domain. IL-22 R alpha 1 associates with either IL-10 R beta or IL-20 R beta to form receptor complexes with distinct ligand selectivities. IL-10 R beta is a shared subunit of the IL-10, -22, -26, -28, and -29 receptors, while IL-20 R beta is a shared subunit of the IL-19, -20, -22R and -24 receptors (1). IL-22 R alpha 1/IL-10 R beta is an IL-22 responsive receptor (2, 3), and IL-22 R alpha 1/IL-20 R beta is an IL-20 or IL-24 responsive receptor (4, 5). IL-22 R alpha 1 contains cytoplasmic motifs for interactions with signal transduction molecules, but formation of ternary complexes with IL-10 R beta or IL-20 R beta and the respective ligands is required for signal transduction (2, 6). IL-22BP functions as a competitive antagonist by binding IL‑22 and preventing its association with IL-22 R alpha 1 (7, 9). Even though it is a receptor for interleukins, IL-22 R alpha 1 is not expressed on hematopoietic cells (6, 10, 11). Instead, IL-22 R alpha 1 expression is restricted to epithelial and stromal cells (6, 10‑13). IL-22 R alpha 1 signaling promotes innate immune responses and wound healing at sites of infection and inflammation. This includes upregulation of antimicrobial, acute phase, proinflammatory, and extracellular matrix proteins as well as proteases (3, 11, 13, 14). IL-22 R alpha 1 signaling also promotes downregulation of proteins involved in keratinocyte differentiation (3, 14). Within the ECD, human IL-22 R alpha 1 shares 78%, 76%, and 83% aa sequence identity with mouse, rat, and canine IL-22 R, respectively. It shares 22%‑25% aa sequence identity with the ECDs of other class II receptors IL-10 R, IL-20 R, and IL-28 R.
- Langer, J.A. et al. (2004) Cytokine Growth Factor Rev. 15:33.
- Xie, M.-H. et al. (2000) J. Biol. Chem. 275:31335.
- Boniface, K. et al. (2005) J. Immunol. 174:3695.
- Dumoutier, L. et al. (2001) J. Immunol. 167:3545.
- Wang, M. et al. (2002) J. Biol. Chem. 277:7341.
- Kotenko, S.V. et al. (2001) J. Biol. Chem. 276:2725.
- Li, J. et al. (2004) Int. Immunopharmacol. 4:693.
- Logsdon, N.J. et al. (2002) J. Interferon Cytokine Res. 22:1099.
- Kotenko, S.V. et al. (2001) J. Immunol. 166:7096.
- Nagalakshmi, M.L. et al. (2004) Int. Immunopharmacol. 4:577.
- Nagalakshmi, M.L. et al. (2004) Int. Immunopharmacol. 4:679.
- Aggarwal, S. et al. (2001) J. Interferon Cytokine Res. 21:1047.
- Wolk, K. et al. (2004) Immunity 21:241.
- Wolk, K. et al. (2006) Eur. J. Immunol. 36:1309.
Product Datasheets
Citations for Human IL-22 R alpha 1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Expression of IL-20 Receptor Subunit beta Is Linked to EAE Neuropathology and CNS Neuroinflammation
Authors: JR Dayton, Y Yuan, LP Pacumio, BG Dorflinger, SC Yoo, MJ Olson, SI Hernández-, MM McMahon, L Cruz-Oreng
Frontiers in Cellular Neuroscience, 2021-09-07;15(0):683687.
Species: Human
Sample Types: Whole Cells, Whole Tissue
Applications: ICC, IHC -
Anti-IL-20 monoclonal antibody inhibited tumor growth in hepatocellular carcinoma
Authors: YS Chiu, CH Hsing, CF Li, CY Lee, YH Hsu, MS Chang
Sci Rep, 2017-12-14;7(1):17609.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Novel role for IL-22 in protection during chronic Mycobacterium tuberculosis HN878 infection
Authors: P Treerat, O Prince, A Cruz-Lagun, M Muñoz-Torr, MA Salazar-Le, M Selman, B Fallert-Ju, TA Reinhardt, JF Alcorn, D Kaushal, J Zuñiga, J Rangel-Mor, JK Kolls, SA Khader
Mucosal Immunol, 2017-03-01;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Interleukin-22 drives the proliferation, migration and osteogenic differentiation of mesenchymal stem cells: a novel cytokine that could contribute to new bone formation in spondyloarthropathies
Authors: Ahmed A El-Zayadi
Rheumatology (Oxford), 2017-03-01;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Secreted recombinant human IL-24 protein inhibits the proliferation of esophageal squamous cell carcinoma Eca-109 cells in vitro and in vivo
Authors: QUNFENG MA, BANGMING JIN, YAO ZHANG, YINAN SHI, CHI ZHANG, DAN LUO et al.
Oncology Reports
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Interleukin-22 is increased in multiple sclerosis patients and targets astrocytes.
Authors: Perriard G, Mathias A, Enz L, Canales M, Schluep M, Gentner M, Schaeren-Wiemers N, Du Pasquier R
J Neuroinflammation, 2015-06-16;12(0):119.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry, IHC -
A novel human interleukin-24 peptide created by computer-guided design contributes to suppression of proliferation in esophageal squamous cell carcinoma Eca-109 cells
Authors: QUNFENG MA, XUEFENG DENG, BANGMING JIN, YAO ZHANG, DAN LUO, HEYU SONG et al.
Oncology Reports
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The crosstalk between IL-22 signaling and miR-197 in human keratinocytes.
Authors: Lerman, Galya, Sharon, Moran, Leibowitz-Amit, Raya, Sidi, Yechezke, Avni, Dror
PLoS ONE, 2014-09-10;9(9):e107467.
Species: Human
Sample Types: Whole Cells
Applications: Western Blot -
Novel mechanism of MDA-7/IL-24 cancer-specific apoptosis through SARI induction.
Authors: Dash R, Bhoopathi P, Das S, Sarkar S, Emdad L, Dasgupta S, Sarkar D, Fisher P
Cancer Res, 2013-11-26;74(2):563-74.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Interleukin-22 suppresses the growth of A498 renal cell carcinoma cells via regulation of STAT1 pathway.
Authors: Zhang F, Shang D, Zhang Y, Tian Y
PLoS ONE, 2011-05-23;6(5):e20382.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
TH17 cytokines induce human airway smooth muscle cell migration.
Authors: Chang Y, Al-Alwan L, Risse PA, Roussel L, Rousseau S, Halayko AJ, Martin JG, Hamid Q, Eidelman DH
J. Allergy Clin. Immunol., 2011-02-22;127(4):1046-53.e1-2.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
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We used this antibody in an in-house ELISA along with pAb (AF2770) and protein standard (2770-LR) to quantify IL-22R alpha1 in human serum and plasma. This combination could not detect IL-22R alpha1 in our samples but generated a good standard curve.
