Human IL-22 R alpha 1 Antibody

IL‑10 secretion Induced by IL‑22 and Neutralization by Human IL‑22 R alpha 1 Antibody. Recombinant Human IL-22 (Catalog # 782-IL) stimulates IL-10 secretion in the COLO 205 human colorectal adenocarcin-oma cell line in a dose-dependent manner (orange line), as measured by the Human IL-10 DuoSet ELISA Development Kit (Catalog # DY217B). IL-10 secretion elicited by Recombinant Human IL-22 (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-22 Ra1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2770). The ND₅₀ is typically 0.2-1 µg/mL.

Detection of Human IL-22R alpha 1 by Flow Cytometry Stimulation of AGS cells with IL-22 leads to the inhibition of H. pylori-induced CCL20 expression.A, AGS cells were stained with an isotype control antibody (dashed line) or an anti-IL-22R1 antibody (solid line) conjugated with allophycocyanin and analyzed by flow cytometry. B, AGS cells were infected with H. pylori (HP) in the presence of various concentration of IL-22 for 24 h and the CCL20 concentration in the culture supernatants was determined by ELISA. C-D, AGS cells were infected with H. pylori in the presence or absence of IL-22 and the CCL20 mRNA in the cells (C) and CCL20 protein in the culture supernatants (D) were determined by real-time PCR and ELISA, respectively. E. AGS cells were pretreated with a neutralizing antibody against IL-22R1 (2 µg/ml) for 1 h followed by the H. pylori infection in the absence or presence of IL-22 for 6 h. The CCL20 concentration in the culture supernatants was determined by ELISA. Data represent the mean ± SEM from three independent experiments. **, p<0.005; *, p<0.02 for H. pylori + IL-22 versus H. pylori only. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0097350), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Human IL-22R alpha 1 by Western Blot IL-22 and IL-22 receptor expression levels increase in the airways of asthmatic subjects. (A-D) Bronchial biopsies were obtained from healthy controls, mild asthmatics and severe asthmatics and stained (A, negative control) for IL-22 expression (in brown) by immunohistochemistry. Scale bar 50 μm. (E) The number of IL-22 positive cells was determined per mm2 of biopsy tissue. n=5 per group, *p<0.05 vs. healthy control. (F) Primary bronchial epithelial cells were obtained from healthy controls, mild asthmatics and severe asthmatics and assessed for IL-22 receptor expression by Western blot. Cells were allowed to grow to confluence, serum starved overnight and stimulated with IL-22, TGF-beta 1 (10 ng/mL each) or both cytokines for 5 days before Western blot analysis. (G) Expression levels of the IL-22 receptor in unstimulated cells were quantified relative to GAPDH expression. n=5 per group, *p<0.05 vs. healthy control. Image collected and cropped by CiteAb from the following publication (https://respiratory-research.biomedcentral.com/articles/10.1186/1465-9921-14-118), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Human IL-22R alpha 1 by Immunohistochemistry IL-22 and IL-22 receptor expression levels increase in the airways of asthmatic subjects. (A-D) Bronchial biopsies were obtained from healthy controls, mild asthmatics and severe asthmatics and stained (A, negative control) for IL-22 expression (in brown) by immunohistochemistry. Scale bar 50 μm. (E) The number of IL-22 positive cells was determined per mm2 of biopsy tissue. n=5 per group, *p<0.05 vs. healthy control. (F) Primary bronchial epithelial cells were obtained from healthy controls, mild asthmatics and severe asthmatics and assessed for IL-22 receptor expression by Western blot. Cells were allowed to grow to confluence, serum starved overnight and stimulated with IL-22, TGF-beta 1 (10 ng/mL each) or both cytokines for 5 days before Western blot analysis. (G) Expression levels of the IL-22 receptor in unstimulated cells were quantified relative to GAPDH expression. n=5 per group, *p<0.05 vs. healthy control. Image collected and cropped by CiteAb from the following publication (https://respiratory-research.biomedcentral.com/articles/10.1186/1465-9921-14-118), licensed under a CC-BY license. Not internally tested by R&D Systems.