Human IL-22 R alpha 1 Biotinylated Antibody

Catalog #: BAF2770 Datasheet / COA / SDS

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BAF2770

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Human IL-22 R alpha 1 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human IL‑22 R alpha 1 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human (rh) IL-20 R alpha, rhIL-20 R beta, and rhIL-22BP is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human IL-22 R alpha 1
Pro18-Thr228
Accession # Q8N6P7

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human IL‑22 R alpha 1 (Catalog # 2770-LR)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-22 R alpha 1

IL-22 receptor, also known as IL-22 R alpha 1 and CRF2-9, is an approximately 65 kDa transmembrane glycoprotein in the type II cytokine receptor family (CRF). IL‑22 R alpha 1 contains a 211 amino acid (aa) extracellular domain (ECD) with two fibronectin type III repeats, and a 323 aa cytoplasmic domain. IL-22 R alpha 1 associates with either IL-10 R beta or IL-20 R beta to form receptor complexes with distinct ligand selectivities. IL-10 R beta is a shared subunit of the IL-10, -22, -26, -28, and -29 receptors, while IL-20 R beta is a shared subunit of the IL-19, -20, -22R and -24 receptors (1). IL-22 R alpha 1/IL-10 R beta is an IL-22 responsive receptor (2, 3), and IL-22 R alpha 1/IL-20 R beta is an IL-20 or IL-24 responsive receptor (4, 5). IL-22 R alpha 1 contains cytoplasmic motifs for interactions with signal transduction molecules, but formation of ternary complexes with IL-10 R beta or IL-20 R beta and the respective ligands is required for signal transduction (2, 6). IL-22BP functions as a competitive antagonist by binding IL‑22 and preventing its association with IL-22 R alpha 1 (7, 9). Even though it is a receptor for interleukins, IL-22 R alpha 1 is not expressed on hematopoietic cells (6, 10, 11). Instead, IL-22 R alpha 1 expression is restricted to epithelial and stromal cells (6, 10‑13). IL-22 R alpha 1 signaling promotes innate immune responses and wound healing at sites of infection and inflammation. This includes upregulation of antimicrobial, acute phase, proinflammatory, and extracellular matrix proteins as well as proteases (3, 11, 13, 14). IL-22 R alpha 1 signaling also promotes downregulation of proteins involved in keratinocyte differentiation (3, 14). Within the ECD, human IL-22 R alpha 1 shares 78%, 76%, and 83% aa sequence identity with mouse, rat, and canine IL-22 R, respectively. It shares 22%‑25% aa sequence identity with the ECDs of other class II receptors IL-10 R, IL-20 R, and IL-28 R.

References

Langer, J.A. et al. (2004) Cytokine Growth Factor Rev. 15:33.
Xie, M.-H. et al. (2000) J. Biol. Chem. 275:31335.
Boniface, K. et al. (2005) J. Immunol. 174:3695.
Dumoutier, L. et al. (2001) J. Immunol. 167:3545.
Wang, M. et al. (2002) J. Biol. Chem. 277:7341.
Kotenko, S.V. et al. (2001) J. Biol. Chem. 276:2725.
Li, J. et al. (2004) Int. Immunopharmacol. 4:693.
Logsdon, N.J. et al. (2002) J. Interferon Cytokine Res. 22:1099.
Kotenko, S.V. et al. (2001) J. Immunol. 166:7096.
Nagalakshmi, M.L. et al. (2004) Int. Immunopharmacol. 4:577.
Nagalakshmi, M.L. et al. (2004) Int. Immunopharmacol. 4:679.
Aggarwal, S. et al. (2001) J. Interferon Cytokine Res. 21:1047.
Wolk, K. et al. (2004) Immunity 21:241.
Wolk, K. et al. (2006) Eur. J. Immunol. 36:1309.

Long Name

Interleukin 22 Receptor

Entrez Gene IDs

58985 (Human); 230828 (Mouse)

Alternate Names

CRF2-9; CRF2-9interleukin 22 receptor; Cytokine receptor class-II member 9; Cytokine receptor family 2 member 9; IL-22 R alpha 1; IL-22 receptor subunit alpha-1; IL22R alpha 1; IL22R; IL22R1; IL22RA1; IL-22Ra1; IL-22R-alpha-1; IL-TIF-R1; interleukin 22 receptor, alpha 1; interleukin-22 receptor subunit alpha-1; zcytoR11