Human IL-22 R alpha 1 PE-conjugated Antibody Summary
Pro18-Thr228
Accession # Q8N6P7
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
View Larger
Detection of IL-22 R alpha 1 in HT-29 Human Cell Line by Flow Cytometry. HT-29 human colon adenocarcinoma cell line was stained with Mouse Anti-Human IL-22 Ra1 PE-conjugated Monoclonal Anti-body (Catalog # FAB2770P, filled histogram) or isotype control antibody (IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.
View Larger
Detection of IL-22 R alpha 1 in COLO 205 Human Cell Line by Flow Cytometry. COLO 205 human colorectal adenocarcinoma cell line was stained with Mouse Anti-Human IL-22 Ra1 PE-conjugated Monoclonal Anti-body (Catalog # FAB2770P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-22 R alpha 1
IL-22 receptor, also known as IL-22 R alpha 1 and CRF2-9, is an approximately 65 kDa transmembrane glycoprotein in the type II cytokine receptor family (CRF). IL-22 R alpha 1 contains a 211 amino acid (aa) extracellular domain (ECD) with two fibronectin type III repeats, and a 323 aa cytoplasmic domain. IL-22 R alpha 1 associates with either IL-10 R beta or IL-20 R beta to form receptor complexes with distinct ligand selectivities. IL-10 R beta is a shared subunit of the IL-10, -22, -26, -28, and -29 receptors, while IL-20 R beta is a shared subunit of the IL-19, -20, -22R and -24 receptors (1). IL-22 R alpha 1/IL-10 R beta is an IL-22 responsive receptor (2, 3), and IL-22 R alpha 1/IL-20 R beta is an IL-20 or IL-24 responsive receptor (4, 5). IL-22 R alpha 1 contains cytoplasmic motifs for interactions with signal transduction molecules, but formation of ternary complexes with IL-10 R beta or IL-20 R beta and the respective ligands is required for signal transduction (2, 6). IL-22BP functions as a competitive antagonist by binding IL-22 and preventing its association with IL-22 R alpha 1 (7, 9). Even though it is a receptor for interleukins, IL-22 R alpha 1 is not expressed on hematopoietic cells (6, 10, 11). Instead, IL-22 R alpha 1 expression is restricted to epithelial and stromal cells (6, 10‑13). IL-22 R alpha 1 signaling promotes innate immune responses and wound healing at sites of infection and inflammation. This includes upregulation of antimicrobial, acute phase, proinflammatory, and extracellular matrix proteins as well as proteases (3, 11, 13, 14). IL-22 R alpha 1 signaling also promotes downregulation of proteins involved in keratinocyte differentiation (3, 14). Within the ECD, human IL-22 R alpha 1 shares 78%, 76%, and 83% aa sequence identity with mouse, rat, and canine IL-22 R, respectively. It shares 22%‑25% aa sequence identity with the ECDs of other class II receptors IL-10 R, IL-20 R, and IL-28 R.
- Langer, J.A. et al. (2004) Cytokine Growth Factor Rev. 15:33.
- Xie, M.-H. et al. (2000) J. Biol. Chem. 275:31335.
- Boniface, K. et al. (2005) J. Immunol. 174:3695.
- Dumoutier, L. et al. (2001) J. Immunol. 167:3545.
- Wang, M. et al. (2002) J. Biol. Chem. 277:7341.
- Kotenko, S.V. et al. (2001) J. Biol. Chem. 276:2725.
- Li, J. et al. (2004) Int. Immunopharmacol. 4:693.
- Logsdon, N.J. et al. (2002) J. Interferon Cytokine Res. 22:1099.
- Kotenko, S.V. et al. (2001) J. Immunol. 166:7096.
- Nagalakshmi, M.L. et al. (2004) Int. Immunopharmacol. 4:577.
- Nagalakshmi, M.L. et al. (2004) Int. Immunopharmacol. 4:679.
- Aggarwal, S. et al. (2001) J. Interferon Cytokine Res. 21:1047.
- Wolk, K. et al. (2004) Immunity 21:241.
- Wolk, K. et al. (2006) Eur. J. Immunol. 36:1309.
Product Datasheets
Citations for Human IL-22 R alpha 1 PE-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
4
Citations: Showing 1 - 4
Filter your results:
Filter by:
-
Interleukin-22 promotes tumor angiogenesis
Authors: Nicholas J. Protopsaltis, Wei Liang, Eric Nudleman, Napoleone Ferrara
Angiogenesis
-
Interleukin-22 Might Act as a Double-Edged Sword in Type 2 Diabetes and Coronary Artery Disease
Authors: Fangchen Gong, Jin Wu, Ping Zhou, Mengyao Zhang, Jingning Liu, Ying Liu et al.
Mediators of Inflammation
-
TH17 cytokines induce human airway smooth muscle cell migration.
Authors: Chang Y, Al-Alwan L, Risse PA, Roussel L, Rousseau S, Halayko AJ, Martin JG, Hamid Q, Eidelman DH
J. Allergy Clin. Immunol., 2011-02-22;127(4):1046-53.e1-2.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
IL-17 and IL-22 mediate IL-20 subfamily cytokine production in cultured keratinocytes via increased IL-22 receptor expression.
Authors: Tohyama M, Hanakawa Y, Shirakata Y, Dai X, Yang L, Hirakawa S, Tokumaru S, Okazaki H, Sayama K, Hashimoto K
Eur. J. Immunol., 2009-10-01;39(10):2779-88.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Human IL-22 R alpha 1 PE-conjugated Antibody
There are currently no reviews for this product. Be the first to review Human IL-22 R alpha 1 PE-conjugated Antibody and earn rewards!
Have you used Human IL-22 R alpha 1 PE-conjugated Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
