Human IL-28B/IFN-lambda 3 Quantikine ELISA Kit

Catalog #: D28B00
5 Citations Datasheet / COA / SDS

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D28B00

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QC222: Quantikine Immunoassay Control Set 1183 Human IL-28B

Human IL-28B/IFN-lambda 3 ELISA Standard Curve

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Citations (5)

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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human IL-28B/IFN-lambda 3 Quantikine ELISA Kit Summary

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 uL), Cell Lysates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Urine (50 uL)

Sensitivity

1.87 pg/mL

Assay Range

15.6 - 1,000 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Urine)

Specificity

Natural and recombinant human IL-28B.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine Human IL-28B/IFN-lambda 3 Immunoassay is a 4.5 hour solid phase ELISA designed to measure IL-28B levels in cell culture supernates, cell lysates, serum, EDTA plasma, and urine. It contains CHO cell-expressed recombinant human IL-28B and antibodies raised against the recombinant protein. Results obtained for naturally occurring human IL-28B showed linear curves that were parallel to the standard curves obtained using the Quantikine Human IL-28B Immunoassay standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-28B.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Urine

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	90.7	279	560	98.6	282	532
Standard Deviation	2.8	10.5	11.9	7.15	19.9	36.1
CV%	3.1	3.8	2.1	7.3	7.1	6.8

Recovery

The recovery of human IL-28B spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	113	83-122
Cell Lysis Buffer (n=4)	96	87-103
EDTA Plasma (n=4)	111	93-124
Serum (n=4)	111	96-124
Urine (n=4)	84	75-96

Linearity

To assess the linearity of the assay, samples spiked with high concentrations of human IL-28B were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Scientific Data

N/A

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Human IL-28B/IFN-lambda 3 ELISA Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-28B/IFN-lambda 3

Human/mouse IL-28A (IFN-lambda 2), IL-28B (IFN-lambda 3), and human IL-29 (IFN-lambda 1) are class II cytokine receptor ligands that are distantly related to members of the IL-10 family (11 - 13% amino acid (aa) sequence identity) and type I IFN family (15 - 19% aa sequence identity). These cytokines exert bioactivities that overlap those of type I IFNs, including antiviral activity and up-regulation of MHC class I antigen expression. The proteins signal through the same heterodimeric receptor complex that is composed of the IL-10 receptor beta (IL-10 R beta) and a novel IL-28 receptor alpha (IL-28 R alpha, also known as IFN-lambda R1). Mouse IL-28 shares 61%, 62%, and 52% aa identity with human IL-28A, IL-28B, and IL-29, respectively.

Long Name:

Interleukin 28B

Entrez Gene IDs:

282617 (Human); 338374 (Mouse)

Alternate Names:

interleukin-28B; IFN-lambda 3; IL28B; IL-28B; IL28C; interferon, lambda 3

Related Research Areas

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human IL-28B/IFN-lambda 3 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Amino acid catabolite markers for early prognostication of pneumonia in patients with COVID-19
Authors: Maeda, R;Seki, N;Uwamino, Y;Wakui, M;Nakagama, Y;Kido, Y;Sasai, M;Taira, S;Toriu, N;Yamamoto, M;Matsuura, Y;Uchiyama, J;Yamaguchi, G;Hirakawa, M;Kim, YG;Mishima, M;Yanagita, M;Suematsu, M;Sugiura, Y;
Nature communications
Species: Human
Sample Types: Serum
Clostridium butyricum-induced ?-3 fatty acid 18-HEPE elicits anti-influenza virus pneumonia effects through interferon-lambda upregulation
Authors: M Hagihara, M Yamashita, T Ariyoshi, S Eguchi, A Minemura, D Miura, S Higashi, K Oka, T Nonogaki, T Mori, K Iwasaki, J Hirai, Y Shibata, T Umemura, H Kato, N Asai, Y Yamagishi, A Ota, M Takahashi, H Mikamo
Cell Reports, 2022-12-13;41(11):111755.
Species: Human
Sample Types: Cell Culture Supernates
Antiviral Activity of Influenza A Virus Defective Interfering Particles against SARS-CoV-2 Replication In Vitro through Stimulation of Innate Immunity
Authors: U Rand, SY Kupke, H Shkarlet, MD Hein, T Hirsch, P Marichal-G, L Cicin-Sain, U Reichl, D Bruder
Cells, 2021-07-11;10(7):.
Species: Human
Sample Types: Cell Culture Supernates
DHX15 is required to control RNA virus-induced intestinal inflammation
Authors: J Xing, X Zhou, M Fang, E Zhang, LJ Minze, Z Zhang
Cell Reports, 2021-06-22;35(12):109205.
Species: Human
Sample Types: Cell Culture Supernates
Symmetric Arginine Dimethylation Is Selectively Required for mRNA Splicing and the Initiation of Type I and Type III Interferon Signaling
Authors: PJ Metz, KA Ching, T Xie, P Delgado Cu, S Niessen, JH Tatlock, K Jensen-Per, BW Murray
Cell Rep, 2020-02-11;30(6):1935-1950.e8.
Species: Human
Sample Types: Cell Culture Supernates