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Human IL-32 Antibody

Catalog #: AF3040
16 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3040
AF3040-SP
Product Details
Citations (16)
FAQs
Supplemental Products
Reviews (1)
Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-32 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-32 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human IL‑32 alpha
Cys2-Lys131
Accession # NP_001012651
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL

Recombinant Human IL‑32 alpha

Recombinant Human IL-32 beta

Recombinant Human IL-32 gamma (Catalog # 4690-IL/CF)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-32

Interleukin 32 (IL-32) is an N-glycosylated cytokine that is upregulated by inflammatory stimulation in monocytes, NK cells, epithelial cells, and pancreatic myofibroblasts (1-5). It cooperates with these stimuli to promote the expression of other proinflammatory molecules such as TNF-alpha, IL-6, IL-1 beta, IL-1 alpha, and CXCL8/IL‑8 (5-7). The longest of several IL-32 splicing variants is the 20-25 kDa gamma isoform which is also known as natural killer cell transcript 4 (NK4) (8, 9). The alpha isoform (IL-32 alpha ) lacks a portion of the putative signal peptide as well as 57 aa from the C-terminal region. IL-32 alpha is less potent than IL-32 beta, gamma, or δ at inducing the expression of proinflammatory molecules in peripheral blood mononuclear cells (PBMC) (8, 10). Neutrophil-derived Proteinase 3 (PR3) cleaves IL-32 alpha between Thr57 and Val58, a cleavage site that is retained in other IL-32 isoforms (11). The N-terminal fragment of PR3-cleaved IL-32 alpha shows increased potency at inducing CXCL2/MIP-2 and CXCL8 expression in PBMC relative to uncleaved IL-32 alpha (11, 12). IL-32 is highly expressed by colonic epithelial cells in inflammatory bowel disease and Crohn’s disease, rheumatoid arthritis synovium, and ductal epithelial cells in chronic pancreatitis and pancreatic cancer (5, 13-15). IL-32 inhibits HIV-1 replication in vitro, and it is elevated in the serum of HIV-1 patients (16, 17).

References
  1. Netea, M.G. et al. (2006) PloS Med. 3:e277.
  2. Nold-Petry, C.A. et al. (2009) Proc. Natl. Acad. Sci. USA 106:3883.
  3. Li, W. et al. (2009) Eur. J. Immunol. 39:1019.
  4. Nishida, A. et al. (2008) Am. J. Physiol. Gastrointest. Liver Physiol. 294:G831.
  5. Shoda, H. et al. (2006) Arthritis Res. Ther. 8:R166.
  6. Netea, M.G. et al. (2005) Proc. Natl. Acad. Sci. USA 102:16309.
  7. Hong, J. et al. (2010) Cytokine 49:171.
  8. Kim, S-H. et al. (2005) Immunity 22:131.
  9. Dahl, C.A. et al. (1992) J. Immunol. 148:597.
  10. Choi, J-D. et al. (2009) Immunology 126:535.
  11. Novick, D. et al. (2006) Proc. Natl. Acad. Sci. USA 103:3316.
  12. Kim, S. et al. (2008) BMB Rep. 41:814.
  13. Shioya, M. et al. (2007) Clin. Exp. Immunol. 149:480.
  14. Joosten, L.A.B. et al. (2006) Proc. Natl. Acad. Sci. USA 103:3298.
  15. Nishida, A. et al. (2009) J. Biol. Chem. 284:17868.
  16. Rasool, S.T. et al. (2008) Immunol. Lett. 117:161.
  17. Nold, M.F. et al. (2008) J. Immunol. 181:557.
Long Name
Interleukin 32
Entrez Gene IDs
9235 (Human)
Alternate Names
IL32; IL-32; IL-32alpha; IL-32beta; interleukin 32; interleukin-32 theta; interleukin-32; natural killer cell transcript 4; Natural killer cells protein 4; NK4; NK4IL-32delta; TAIF; TAIFa; TAIFb; TAIFc; TAIFd; TAIFIL-32gamma; Tumor necrosis factor alpha-inducing factor

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Citations for Human IL-32 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

16 Citations: Showing 1 - 10
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  1. The pro-tumorigenic cytokine IL-32 has a high turnover in multiple myeloma cells due to proteolysis regulated by oxygen-sensing cysteine dioxygenase and deubiquitinating enzymes
    Authors: Martin Kastnes, Kristin Roseth Aass, Siri Anshushaug Bouma, Charlotte Årseth, Muhammad Zahoor, Mariia Yurchenko et al.
    Frontiers in Oncology
  2. β-Glucan-Induced Trained Immunity Protects against Leishmania braziliensis Infection: a Crucial Role for IL-32
    Authors: JC Dos Santos, AM Barroso de, MV Teodoro Si, B Cirovic, LCJ de Bree, MSMA Damen, SJCFM Moorlag, RS Gomes, MM Helsen, M Oosting, ST Keating, A Schlitzer, MG Netea, F Ribeiro-Di, LAB Joosten
    Cell Rep, 2019-09-03;28(10):2659-2672.e6.
    Species: Transgenic Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  3. Genetic variant in IL-32 is associated with the ex vivo cytokine production of anti-TNF treated PBMCs from rheumatoid arthritis patients
    Authors: MSMA Damen, K Schraa, L Tweehuysen, AA den Broede, MG Netea, CD Popa, LAB Joosten
    Sci Rep, 2018-09-19;8(1):14050.
    Species: Human
    Sample Types: Cell Culture Supernates
  4. Allele-specific long-distance regulation dictates IL-32 isoform switching and mediates susceptibility to HIV-1
    Authors: RJ Palstra, E de Crignis, MD Röling, T van Staver, TW Kan, W van Ijcken, YM Mueller, PD Katsikis, T Mahmoudi
    Sci Adv, 2018-02-21;4(2):e1701729.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Interleukin (IL)-32beta-mediated CCAAT/enhancer-binding protein alpha (C/EBPalpha) phosphorylation by protein kinase Cdelta (PKCdelta) abrogates the inhibitory effect of C/EBPalpha on IL-10 production.
    Authors: Kang J, Park Y, Kim M, Lee D, Bak Y, Ham S, Park S, Hong J, Yoon D
    J Biol Chem, 2013-06-30;288(33):23650-8.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  6. Role of interleukin-32 in Helicobacter pylori-induced gastric inflammation.
    Authors: Sakitani K, Hirata Y, Hayakawa Y, Serizawa T, Nakata W, Takahashi R, Kinoshita H, Sakamoto K, Nakagawa H, Akanuma M, Yoshida H, Maeda S, Koike K
    Infect Immun, 2012-08-13;80(11):3795-803.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Inhibition of IL-32 activation by alpha-1 antitrypsin suppresses alloreactivity and increases survival in an allogeneic murine marrow transplantation model.
    Authors: Marcondes AM, Li X, Tabellini L, Bartenstein M, Kabacka J, Sale GE, Hansen JA, Dinarello CA, Deeg HJ
    Blood, 2011-09-06;118(18):5031-9.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Inflammation-dependent secretion and splicing of IL-32{gamma} in rheumatoid arthritis.
    Authors: Heinhuis B, Koenders MI, van de Loo FA, Netea MG, van den Berg WB, Joosten LA
    Proc. Natl. Acad. Sci. U.S.A., 2011-03-07;108(12):4962-7.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  9. Protection from RNA and DNA viruses by IL-32.
    Authors: Zepp JA, Nold-Petry CA, Dinarello CA, Nold MF
    J. Immunol., 2011-02-23;186(7):4110-8.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Electrochemiluminescent Assay
  10. Native IL-32 is released from intestinal epithelial cells via a non-classical secretory pathway as a membrane-associated protein.
    Authors: Hasegawa H, Thomas HJ, Schooley K, Born TL
    Cytokine, 2011-01-01;53(1):74-83.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  11. Interleukin-32 expression in the pancreas.
    Authors: Nishida A, Andoh A, Inatomi O, Fujiyama Y
    J. Biol. Chem., 2009-04-21;284(26):17868-76.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  12. Epithelial overexpression of interleukin-32alpha in inflammatory bowel disease.
    Authors: Shioya M, Nishida A, Yagi Y, Ogawa A, Tsujikawa T, Kim-Mitsuyama S, Takayanagi A, Shimizu N, Fujiyama Y, Andoh A
    Clin. Exp. Immunol., 2007-06-22;149(3):480-6.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  13. IL-32 is induced by activation of toll-like receptors in multiple myeloma cells
    Authors: Kristin Roseth Aass, Synne Stokke Tryggestad, Robin Mjelle, Martin H. Kastnes, Tonje Marie Vikene Nedal, Kristine Misund et al.
    Frontiers in Immunology
  14. Hypoxia promotes IL-32 expression in myeloma cells, and high expression is associated with poor survival and bone loss
    Authors: Muhammad Zahoor, Marita Westhrin, Kristin Roseth Aass, Siv Helen Moen, Kristine Misund, Katarzyna Maria Psonka-Antonczyk et al.
    Blood Advances
  15. Interplay between thyroid cancer cells and macrophages: effects on IL-32 mediated cell death and thyroid cancer cell migration
    Authors: Yvette J. E. Sloot, Katrin Rabold, Thomas Ulas, Dennis M. De Graaf, Bas Heinhuis, Kristian Händler et al.
    Cell Oncol (Dordr)
  16. Intracellular IL-32 regulates mitochondrial metabolism, proliferation, and differentiation of malignant plasma cells
    Authors: Kristin Roseth Aass, Robin Mjelle, Martin H. Kastnes, Synne S. Tryggestad, Luca M. van den Brink, Ingrid Aass Roseth et al.
    iScience

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Human IL-32 Antibody
By Anonymous on 03/08/2019
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Adult lung Species: Human

Did not work with human lung sections without retreival or with citrate retreival - IgG was used as control.
***Bio-Techne Response: Thank you for reviewing our product. We are sorry to hear that this antibody did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.***

Immunocytochemistry/Immunofluorescence Human IL-32 Antibody AF3040