Human IL-34 Antibody

Catalog #: MAB5265
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5265
MAB5265-SP

Cell Proliferation Induced by IL‑34 and Neutralization by Human IL‑34 Antibody.

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All IL-34 Antibodies

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Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-34 Antibody Summary

Species Reactivity

Human

Specificity

Detects recombinant human IL-34 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse IL-34 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 578416

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human IL-34
Asn21-Pro242
Accession # Q6ZMJ4

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<1.0 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration

Sample

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

2.5 µg/10⁶ cells

Human IL-34 transfected NS0 cells fixed with paraforaldehyde and permeabilized with saponin

Neutralization

Measured by its ability to neutralize IL‑34-induced proliferation in human peripheral blood monocytes. Lin, H. et al. (2008) Science 320:807. The Neutralization Dose (ND₅₀) is typically 0.01-0.05 µg/mL in the presence of 10 ng/mL Recombinant Human IL‑34.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Cell Proliferation Induced by IL‑34 and Neutralization by Human IL‑34 Antibody. Recombinant Human IL-34 (Catalog # 5265-IL) stimulates proliferation in human peripheral blood monocytes in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-34 (10 ng/mL) is neutralized (green line) by increasing concentrations of Human IL-34 Monoclonal Antibody (Catalog # MAB5265). The ND₅₀ is typically 0.01-0.05 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-34

Interleukin 34 (IL-34; also known as uncharacterized protein C16orf77) is secreted as a homodimer consisting of 39 kDa monomers (1). It belongs to no known cytokine family. Human IL-34 is synthesized as a 242 amino acid (aa) precursor that contains a 20 aa signal sequence and a 222 aa mature chain. The mature chain contains one potential site of N-linked glycosylation. Human IL-34 is 71% identical to mouse IL-34 on the amino acid level (1). IL-34 is expressed in various tissues, including the heart, brain, liver, kidney, spleen, thymus, testes, ovary, small intestine, prostate, and colon, and is most abundant in the spleen (1). The receptor for IL-34 is colony-stimulating factor 1 receptor (CSF-1R) (1). IL-34 stimulates monocyte proliferation (1). In functional studies, IL-34, like CSF-1, the other ligand for CSF-1R, stimulated phosphorylation of extracellular signal-regulated kinase-1 and -2 (ERK1/2) in human monocytes (1). In addition, IL-34 promoted the formation of the colony-forming unit-macrophage (CFU-M), a macrophage progenitor, in human bone marrow cultures (1).

References

Lin, H. et al. (2008) Science 320:807.

Long Name

Interleukin 34

Entrez Gene IDs

146433 (Human); 76527 (Mouse)

Alternate Names

C16orf77; IL34; IL-34; interleukin 34

Product Datasheets

Product Datasheet

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Related Research Areas

Citation for Human IL-34 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

IL-34 is a Treg-specific cytokine and mediates transplant tolerance.
Authors: Bezie S, Picarda E, Ossart J, Tesson L, Usal C, Renaudin K, Anegon I, Guillonneau C
J Clin Invest, 2015-09-21;125(10):3952-64.
Species: Human, Rat
Sample Types: Whole Cells
Applications: Neutralization