Human IL-36 alpha /IL-1F6 Biotinylated Antibody

Catalog #: BAF1078 Datasheet / COA / SDS

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BAF1078

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Human IL-36 alpha /IL-1F6 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human IL‑36 alpha /IL‑1F6 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human (rh) rhIL-36Ra, rhIL‑37, rhIL-1 alpha, rhIL-1 beta and rhIL-18 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human IL‑36 alpha /IL‑1F6
Met1-Phe158
Accession # Q9UHA7

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human IL‑36 alpha /IL‑1F6 (Catalog # 1078-IL)

Immunohistochemistry

5-15 µg/mL

Immersion fixed paraffin-embedded sections of human tonsil

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-36 alpha/IL-1F6

Human interleukin 1 family member #6 [IL-1F6; also FIL-1 epsilon (epsilon)] is a member of the IL-1 family of proteins (1‑3). IL-1 family members include IL-1 beta, IL-1 alpha,
IL‑1ra, IL-18 and IL-1F5 through F10 (4). All family members show a 12 beta -strand, beta -trefoil configuration, and all family members are believed to have arisen from a common ancestral gene that has undergone multiple duplications (4). IL-1F6 is synthesized as a 158 amino acid (aa) protein that contains no signal sequence, no prosegment and no potential N-linked glycosylation site(s) (1‑3). It appears to be actively secreted (1). When found in cell lysate, it presents as an 18 kDa monomer (2). Human to mouse, full length IL-1F6 has 54% aa identity. Within the family, IL-1F6 is 30% aa identical to IL-1ra, and 27%, 31%, 36%, 46%, 57% and 28% aa identical to IL-1 beta, IL-1F5, F7, F8, F9 and F10, respectively. Cells reported to express IL-1F6 include monocytes, B cells and T cells (1, 4). Notably, IL-1F6 is the only novel IL-1 family member found to be expressed on T-cells. The receptor for IL-1F6 is reported to be a combination of IL-1 Rrp2 and IL-1 RAcP (5). Recombinant
IL‑1F6, along with IL-1F8 and IL-1F9, has been shown to act as an agonist by activating the pathway involving NF-kappa B and MAPK in an IL-1 Rrp2 dependent manner. This suggests that IL-1F6 may signal in a similar fashion to IL-1 and IL-18 in having a binding receptor which upon ligation, recruits a second receptor as a signaling component, forming an active heterodimeric receptor complex.

References

Smith, D.E. et al. (2000) J. Biol. Chem. 275:1169.
Kumar, S. et al. (2000) J. Biol. Chem. 275:10308.
Nicklin, M.J.H. et al. (2002) Genomics. 79:718.
Dunn, E. et al. (2001) Trends Immunol. 22:533.
Towne, J.E. et al. (2004) J. Biol. Chem. 279:13677.

Long Name

Interleukin 36 alpha/Interleukin 1 Family 6

Entrez Gene IDs

27179 (Human); 54448 (Mouse)

Alternate Names

FIL1 epsilon; FIL1; FIL1(EPSILON); FIL1E; IL-1 epsilon; IL1(EPSILON); IL1E; IL-1E; IL-1F6 (FIL-1-epsilon); IL1F6; IL-1F6; IL36 alpha; IL-36 alpha; IL36A; interleukin 1 family, member 6 (epsilon); interleukin 1, epsilon; Interleukin 36, Alpha; Interleukin-1 epsilon; interleukin-1 family member 6; Interleukin-36 Alpha