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Human IL-36Ra/IL-1F5 Antibody

Catalog #: AF1275
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1275
AF1275-SP
Product Details
Citations (3)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-36Ra/IL-1F5 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-36Ra/IL-1F5 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) IL-1ra is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human IL-36Ra/IL-1F5
Val2-Asp155
Accession # Q9UBH0
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human IL-36Ra/IL-1F5 (Catalog # 1275-IL)
Blockade of Receptor-ligand Interaction
In a functional ELISA, 0.5-2 µg/mL of this antibody will block 50% of the binding of 5 μg/mL of Recombinant Human IL-1 Rrp2 Fc Chimera or Recombinant Mouse IL-1 Rrp2 (Catalog # 872-RP or 2354-RP) to immobilized Recombinant Human IL-36Ra/IL-1F5 or Recombinant Mouse IL-36Ra/IL-1F5, respectively (Catalog # 1275-IL or 2714-ML) coated at 1 µg/mL (100 µL/well). At 100 μg/mL, this antibody will block >90% of the binding.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-36Ra/IL-1F5

Human Interleukin-36 receptor antagonist protein [IL36Ra; also named interleukin 1 family member 5, IL-1F5, FIL-1δ (delta), IL36RN, IL-1HY1, IL-1H3, and IL-1L1] is a member of the IL-1 family of proteins (1‑6). IL-1 family members include IL-1 beta, IL-1 alpha, IL-1ra, IL-18 and IL-1F5-F10 (6, 7). All family members show a 12 beta -strand, beta -trefoil configuration, and all family members are believed to have arisen from a common ancestral gene that underwent multiple duplications (7). The human IL-1F5 gene is in closest proximity to the gene for IL-1ra and is likely a relatively recent duplication of the IL-1ra gene (2, 3). IL-1F5 is synthesized as a 155 amino acid (aa) protein that contains no signal sequence, no prosegment and no potential N-linked glycosylation site(s) (2‑5). Nevertheless, it appears to be secreted as a 17 kDa monomer (5). There is an alternate start site that potentially gives rise to an alternate splice form (5). The translated product, however, has a premature stop codon, resulting in a truncated 16 aa peptide. Human to mouse, full length IL-1F5 has 90% aa identity. Within the family, IL-1F5 is 50% aa identical to IL-1ra, and 32%, 31%, 35%, 37%, 32% and 42% aa identical to IL-1 beta, IL-1F6, F7, F8, F9 and F10, respectively. Cells reported to express IL-1F5 include monocytes, B cells, dendritic cells/Langerhans cells, keratinocytes, and gastric fundus Parietal and Chief cells (1, 8). The receptor for IL-1F5 has not been positively identified. Indirect evidence suggests it is IL-1 Rrp2 and/or IL-1 RAcP (9). In either case, activity association with receptor binding is also unclear. It was initially reported to be an antagonist of IL‑1F9 activity (4, 7). This would be consistent with its hypothesized relationship to IL-1ra. Studies, however, find IL-1F5 antagonist activity difficult to demonstrate (9).

References
  1. Smith, D. E. et al. (2000) J. Biol. Chem. 275:1169.
  2. Kumar, S. et al. (2000) J. Biol. Chem. 275:10308.
  3. Mulero, J.J. et al. (1999) Biochem. Biophys. Res. Commun. 263:702.
  4. Nicklin, M.J.H. et al. (2002) Genomics. 79:718.
  5. Barton, J.L. et al. (2000) Eur. J. Immunol. 30:3299.
  6. Dinarello, C. et al. (2010)  Nature Immunology 11. 973.
  7. Dunn, E. et al. (2001) Trends Immunol. 22:533.
  8. Debets, R. et al. (2001) J. Immunol. 167:1440.
  9. Towne, J.E. et al. (2004) J. Biol. Chem. 279:13677.
Long Name
Interleukin 36 Receptor Antagonist/Interleukin 1 Family 5
Entrez Gene IDs
26525 (Human); 54450 (Mouse)
Alternate Names
FIL1 delta; FIL1; FIL1-delta, IL-1RP3, IL-1L1, IL-1-delta); FIL1DIL-1 delta; IL1F5 (Canonical product IL-1F5a); IL1F5; IL-1HY1; IL-1L1; IL-1RP3; IL36Ra; IL-36Ra; IL36RN; interleukin 1 family, member 5 (delta); interleukin 1, delta; Interleukin 36 Receptor Antagonist; interleukin-1 family member 5; Interleukin-1 HY1; Interleukin-1 receptor antagonist homolog 1; Interleukin-1-like protein 1; Interleukin-36 Receptor Antagonist

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Citations for Human IL-36Ra/IL-1F5 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Hepatokine Fetuin B expression is regulated by leptin-STAT3 signalling and associated with leptin in obesity
    Authors: D Wang, M Wu, X Zhang, L Li, M Lin, X Shi, Y Zhao, C Huang, X Li
    Scientific Reports, 2022-07-27;12(1):12869.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  2. A novel IL36RN/IL1F5 homozygous nonsense mutation, p.Arg10X, in a Japanese patient with adult-onset generalized pustular psoriasis.
    Authors: Sugiura, K, Takeichi, T, Kono, M, Ogawa, Y, Shimoyama, Y, Muro, Y, Akiyama, M
    Br J Dermatol, 2012-06-20;167(3):699-701.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  3. IL-1F5 mediates anti-inflammatory activity in the brain through induction of IL-4 following interaction with SIGIRR/TIR8.
    Authors: Costelloe C, Watson M, Murphy A, McQuillan K, Loscher C, Armstrong ME, Garlanda C, Mantovani A, O'Neill LA, Mills KH, Lynch MA
    J. Neurochem., 2008-02-18;105(5):1960-9.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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