Human IL-38/IL-1F10 Antibody

Catalog #: MAB2427
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2427
MAB2427-SP

Product Details

Citations (1)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-38/IL-1F10 Antibody Summary

Species Reactivity

Human

Specificity

Detects human IL‑38/IL‑1F10 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 50% cross-reactivity with recombinant mouse IL-1F10 is observed and no cross-reactivity with recombinant human (rh) IL-1 alpha /IL-1F1 rhIL-1 beta /IL-1F2, rhIL-1ra/IL-1F3, rhIL-18/IL-1F4, rhIL-36Ra/IL‑1F5, rhIL-36 alpha /IL‑1F6, rhIL‑1F7/FIL1 zeta, rhIL-36 beta /IL-1F8, or rhIL-36 gamma /IL‑1F9 is observed.

Source

Monoclonal Rat IgG_2B Clone # 316709

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human IL‑38/IL‑1F10
Met1-Trp152
Accession # Q8WWZ1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human IL‑38/IL‑1F10

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-38/IL-1F10

Human IL-38, previously called IL-1F10 or IL-1HY2, is a secreted cytokine belonging to the IL-1 family. It is synthesized as a 152 amino acid mature protein that lacks an identifiable signal sequence. It binds to soluble IL-1 RI. Human IL-38/IL-1F10 shares 82% aa sequence identity with mouse IL-38/IL-1F10. Human IL-38/IL-1F10 also shares 39% and 27% aa sequence identity with IL-1ra/IL-1F3 and IL-1 beta /IL-1F2, respectively.

Long Name

Interleukin 1 Family Member 10

Entrez Gene IDs

84639 (Human); 215274 (Mouse); 102125744 (Cynomolgus Monkey)

Alternate Names

FIL1- theta; FKSG75; IL1F10; IL-1HY2; IL38; IL-38; interleukin 1 family, member 10 (theta); UNQ6119/PRO20041

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human IL-38/IL-1F10 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Interleukin-1 family cytokines and their regulatory proteins in normal pregnancy and pre-eclampsia.
Authors: Southcombe J, Redman C, Sargent I, Granne I
Clin Exp Immunol, 2015-06-29;181(3):480-90.
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot