Human IL-4 Antibody

Name: Human IL-4 Antibody
Brand: R&D Systems
SKU: AB-204-NA

Catalog #: AB-204-NA
2 Citations Datasheet / COA / SDS

Discontinued Product

AB-204-NA has been discontinued.

View all IL-4 products.

Cell Proliferation Induced by IL‑4 and Neutralization by Human IL‑4 Antibody.

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All IL-4 Antibodies

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Citations (2)

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Human IL-4 Antibody Summary

Species Reactivity

Human

Specificity

Detects human IL-4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 25% cross-reactivity with recombinant rhesus monkey IL-4 is observed, and less than 1% cross-reactivity with recombinant mouse IL-4, recombinant rat IL-4, recombinant feline IL-4, recombinant canine IL-4, recombinant porcine IL-4, and recombinant bovine IL-4 is observed.

Source

Polyclonal Goat IgG

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human IL‑4 (R&D Systems, Catalog # 204-IL)
His25-Ser153
Accession # NP_000580

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human IL‑4 (Catalog # 204-IL)

Neutralization

Measured by its ability to neutralize IL‑4-induced proliferation in the TF‑1 human erythroleukemic cell line [Kitamura, T. et al. (1989) J. Cell Physiol. 140:323]. The Neutralization Dose (ND₅₀) is typically 0.05-0.2 µg/mL in the presence of 0.5 ng/mL Recombinant Human IL‑4.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Cell Proliferation Induced by IL‑4 and Neutralization by Human IL‑4 Antibody. Recombinant Human IL-4 (Catalog # 204-IL) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-4 (0.5 ng/mL) is neutralized (green line) by increasing concen-trations of Goat Anti-Human IL-4 Polyclonal Antibody (Catalog # AB-204-NA). The ND₅₀ is typically 0.05 - 0.2 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 1 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-4

Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13-18 kDa Th2 cytokine that shows pleiotropic effects during immune responses. It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha -helix structure. Human IL-4 is synthesized with a 24 aa signal sequence. Alternate splicing generates an isoform with a 16 aa internal deletion. Mature human IL-4 shares 55%, 39%, and 43% aa sequence identity with bovine, mouse, and rat IL-4, respectively. Human, mouse, and rat IL-4 are species-specific in their activities. IL-4 exerts its effects through two receptor complexes. The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and -21). The type II receptor on nonhematopoietic cells consists of IL-4 R alpha and IL-13 R alpha 1. The type II receptor also transduces IL‑13 mediated signals. IL-4 is primarily expressed by Th2-biased CD4⁺ T cells, mast cells, basophils, and eosinophils. It promotes cell proliferation, survival, and immunoglobulin class switch to IgG4 and IgE in human B cells, acquisition of the Th2 phenotype by naïve CD4⁺ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells. IL-4 plays a dominant role in the development of allergic inflammation and asthma.

Long Name

Interleukin 4

Entrez Gene IDs

3565 (Human); 16189 (Mouse); 287287 (Rat); 397225 (Porcine); 280824 (Bovine); 403785 (Canine); 574281 (Primate); 100302454 (Rabbit)

Alternate Names

B cell growth factor 1; BCDF; B-cell stimulatory factor 1; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; IL4; IL-4; IL-4B_cell stimulatory factor 1; IL4E12; interleukin 4; interleukin-4; Lymphocyte stimulatory factor 1; MGC79402; pitrakinra

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human IL-4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Nucleolar protein NOP2/NSUN1 suppresses HIV-1 transcription and promotes viral latency by competing with Tat for TAR binding and methylation
Authors: W Kong, A Biswas, D Zhou, G Fiches, K Fujinaga, N Santoso, J Zhu
PLoS Pathog., 2020-03-16;16(3):e1008430.
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture
A Novel Bromodomain Inhibitor Reverses HIV-1 Latency through Specific Binding with BRD4 to Promote Tat and P-TEFb Association
Authors: H Huang, S Liu, M Jean, S Simpson, H Huang, M Merkley, T Hayashi, W Kong, I Rodríguez-, X Zhang, HO Yosief, H Miao, J Que, JJ Kobie, J Bradner, NG Santoso, W Zhang, J Zhu
Front Microbiol, 2017-06-07;8(0):1035.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization