Human IL-5 Antibody Summary
Ile20-Ser134
Accession # P05113
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Cell Proliferation Induced by IL‑5 and Neutralization by Human IL‑5 Antibody. Recombinant Human IL-5 (Catalog # 205-IL) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-5 (1.25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-5 Polyclonal Antibody (Catalog # AB-205-NA). The ND50 is typically 8-10 µg/mL.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-5
Interleukin 5 is a T cell-derived factor that promotes the proliferation, differentiation and activation of eosinophils. In mice, IL-5 has also been shown to be a growth and differentiation factor for B cells. Various names previously used to describe IL-5 include: T cell replacing factor (TRF), B cell growth factor II (BCGFII), B cell differentiation factor μ (BCDF μ), eosinophil differentiation factor (EDF) and eosinophil colony-stimulating factor (Eo-CSF). Biologically active IL-5 is a disulfide-linked homodimer. The cDNAs for murine and human IL-5 encode precursor proteins with signal peptides that are cleaved to form mature proteins containing 113 and 115 amino acid residues, respectively. Murine and human IL-5 are 70% identical in their amino acid sequences and show species cross‑reactivity. The genes for human and mouse IL-5 have been mapped to chromosome 5 and chromosome 11, respectively; closely linked to the genes for IL-3, IL-4 and GM-CSF.
IL-5 exerts its activity on target cells by binding to specific cell surface receptors. The functional high‑affinity receptor for human IL-5 has been shown to be composed of a low-affinity IL-5 binding alpha -subunit and a non-binding common beta -subunit that is shared with the high‑affinity receptors for GM‑CSF and IL-3.
Product Datasheets
Citation for Human IL-5 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Inhibition of interleukin-5 induced false positive anti-drug antibody responses against mepolizumab through the use of a competitive blocking antibody
Authors: Daniel Sikkema
J. Immunol. Methods, 2016-11-24;0(0):.
Applications: Competitive Binding Assay
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