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Human IL-5 PE-conjugated Antibody

Catalog #: IC605P
2 Citations Datasheet / COA / SDS
Newer Version Available: IC605G
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Discontinued Product

IC605P has been discontinued and is replaced by IC605U. IC605N. IC605T. IC605S. IC605G.

Detection of IL-5 in Human Peripheral Blood Mononuclear Cells by Flow Cytometry.
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Citations (2)
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Human IL-5 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-5 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse IL‑5 is observed.
Source
Monoclonal Mouse IgG1 Clone # 9906
Purification
Protein A or G purified from ascites
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑5
Ile20-Ser134
Accession # P05113
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
0.5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of IL-5 antibody in Human Peripheral Blood Mononuclear Cells antibody by Flow Cytometry. View Larger

Detection of IL-5 in Human Peripheral Blood Mononuclear Cells by Flow Cytometry. Resting (panel A) and PMA/ionomycin-activated (panel B) human peripheral blood mononuclear cells were stained with Mouse Anti-Human IL-5 PE-conjugated Monoclonal Antibody (Catalog # IC605P) and Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (Catalog # FAB100A). Quadrant markers were set based on control antibody staining (Catalog # IC002P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: IL-5

Interleukin-5 (IL-5) is a 20-21 kDa secreted glycoprotein that belongs to the alpha -helical group of cytokines (1-3). Unlike other family members, it is present as a covalently linked antiparallel dimer (4, 5). The cDNA for human IL-5 encodes a signal peptide and a 115 amino acid (aa) mature protein that contains both N- and O-linked carbohydrate. Mature human IL-5 shares 70%, 70%, 62%, 71%, 70% and 66%, aa sequence identity with mouse, rat, canine, equine, feline and porcine IL-5, respectively. IL-5 is primarily produced by CD4+ Th2 cells, but also by activated eosinophils, mast cells, EBV-transformed B cells, group 2 ILCs, macrophages, and IL‑2‑stimulated invariant natural killer T cells (iNKT) (1, 3, 6-9). IL-5 increases production and mobilization of eosinophils and CD34+ progenitors from the bone marrow and causes maturation of eosinophil precursors outside the bone marrow (1, 6, 10). The receptor for human IL-5, mainly expressed by eosinophils, but also found on basophils and mast cells, consists of a unique ligand-binding subunit (IL-5 R alpha ) and a shared signal‑transducing subunit, beta c (3, 6, 11). IL-5 R alpha first binds IL-5 at low affinity, then associates with preformed beta c dimers, forming a high-affinity receptor (12). IL-5 also binds proteoglycans, potentially enhancing its activity (13). Soluble forms of IL-5 R alpha   antagonize IL-5 and can be found in vivo (10, 14). In humans, IL-5 primarily affects cells of the eosinophilic lineage, and promotes their differentiation, maturation, activation, migration and survival, while in mice IL-5 also enhances Ig class switching and release from B1 cells (1 ‑ 3, 9, 10, 15, 16). IL-5 also promotes differentiation of basophils and primes them for histamine and leukotriene release (17).

References
  1. Rosenberg, H. F. et al. (2007) J. Allergy Clin. Immunol. 119:1303.
  2. Takatsu, K. (2014) Front. Immunol. 5:369.
  3. Martinez-Moczygemba, M. and D. P. Huston (2003) J. Allergy Clin. Immunol. 112:653.
  4. Minamitake, Y. et al. (1990) J. Biochem. 107:292.
  5. McKenzie, A. N. et al. (1991) Mol. Immunol. 28:155.
  6. Shakoory, B. et al. (2004) J. Interferon Cytokine Res. 24:271.
  7. Hazenberg, M.D.  and H. Spits (2014) Blood 124:700.
  8. Sakuishi, K. et al. (2007) J. Immunol. 179:3452.
  9. Li, X. et al. (2016) Biochem. Biophys. Res. Commun. 473:939.
  10. Cameron, L. et al. (2000) J. Immunol. 164:1538.
  11. Broughton, S.E. et al. (2015) Cytokine 74:247.
  12. Zaks-Zilberman, M. et al. (2008) J. Biol. Chem. 283:13398.
  13. Lipscombe, R. et al. (1998) J. Leukocyte Biol. 63:342.
  14. Tavernier, J. et al. (2000) Blood 95:1600.
  15. Kopf, M. et al. (1996) Immunity 4:15.
  16. Horikawa, K. and K. Takatsu (2006) Immunology 118:497.
  17. Denburg, J. A. et al. (1991) Blood 77:1462.
Long Name
Interleukin 5
Entrez Gene IDs
3567 (Human); 16191 (Mouse); 24497 (Rat); 397409 (Porcine); 280825 (Bovine); 403790 (Canine); 493803 (Feline)
Alternate Names
BCDF mu; B-cell differentiation factor I; BCGFII; EDF; Eo-CSF; Eosinophil differentiation factor; IL5; IL-5; IL-5T-cell replacing factor; interleukin 5 (colony-stimulating factor, eosinophil); interleukin-5; TRF; TRFB cell differentiation factor I

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Citations for Human IL-5 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. T-cell activation during exacerbations: a longitudinal study in refractory asthma.
    Authors: Mamessier E, Nieves A, Lorec AM, Dupuy P, Pinot D, Pinet C, Vervloet D, Magnan A
    Allergy, 2008-09-01;63(9):1202-10.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Human CD4(+) T cells recognize an epitope within alpha-fetoprotein sequence and develop into TGF-beta-producing CD4(+) T cells.
    Authors: Alisa A, Boswell S, Pathan AA, Ayaru L, Williams R, Behboudi S
    J. Immunol., 2008-04-01;180(7):5109-17.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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