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Human IL-6 Antibody

Catalog #: AB-206-NA
15 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AB-206-NA
Cell Proliferation Induced by IL‑6 and Neutralization by Human IL‑6 Antibody.
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Citations (15)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-6 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-6 in direct ELISAs and Western blots. In these formats, less than 5% cross-reactivity with recombinant mouse IL‑6, recombinant bovine IL-6, recombinant porcine IL-6, recombinant equine IL-6, recombinant feline IL-6, recombinant rat IL-6, recombinant cotton rat IL-6, recombinant rabbit IL-6, and recombinant viral IL-6 is observed.
Source
Polyclonal Goat IgG
Purification
Protein A or G purified
Immunogen
E. coli-derived recombinant human IL-6
Pro29-Met212
Accession # P05231
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human IL‑6 (Catalog # 206-IL)
Simple Western
50 µg/mL
PBMC conditioned media
Neutralization
Measured by its ability to neutralize IL‑6-induced proliferation in the T1165.85.2.1 mouse plasmacytoma cell line [Nordan, R.P. and M. Potter (1986) Science 233:566]. The Neutralization Dose (ND50) is typically 0.05-0.15 µg/mL in the presence of 2.5 ng/mL Recombinant Human IL‑6.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by IL‑6 and Neutralization by Human IL‑6 Antibody. View Larger

Cell Proliferation Induced by IL‑6 and Neutralization by Human IL‑6 Antibody. Recombinant Human IL-6 (Catalog # 206-IL) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-6 (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-6 Polyclonal Antibody (Catalog # AB-206-NA). The ND50 is typically 0.05-0.15 µg/mL.

Simple Western View Larger

Detection of Human IL‑6 by Simple WesternTM. Simple Western lane view shows PBMC conditioned media untreated (-) or treated (+) with PHA, loaded at 0.2 mg/mL. A specific band was detected for IL‑6 at approximately 31 kDa (as indicated) using 50 µg/mL of Goat Anti-Human IL‑6 Polyclonal Antibody (Catalog # AB-206-NA). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 1 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-6

Interleukin-6 (IL-6) is a pleiotropic, alpha -helical, phosphorylated and variably glycosylated cytokine that plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. Mature human IL-6 is 183 amino acids (aa) in length expressed as a 22-28 kDA molecular weight protein. IL-6 shares 39% aa sequence identity with mouse and rat IL-6. Alternative splicing generates several isoforms with internal deletions, some of which exhibit antagonistic properties. IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R alpha) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R alpha, triggering IL-6 R alpha association with gp130 and gp130 dimerization. gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130-expressing cells that lack cell surface IL-6 R alpha. Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 R alpha is predominantly restricted to hepatocytes, monocytes, and resting lymphocytes. Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R alpha but not from other cytokines that use gp130 as a co-receptor. IL-6, along with TNF-alpha and IL-1, function to drive the acute inflammatory response and the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. When dysregulated, it contributes to chronic inflammation in obesity, insulin resistance, inflammatory bowel disease, arthritis, sepsis, and atherosclerosis. IL-6 can also function as an anti-inflammatory molecule, as in skeletal muscle where it is secreted in response to exercise. In addition, it enhances hematopoietic stem cell proliferation and the differentiation of Th17 cells, memory B cells, and plasma cells.

Long Name
Interleukin 6
Entrez Gene IDs
3569 (Human); 16193 (Mouse); 24498 (Rat); 399500 (Porcine); 280826 (Bovine); 403985 (Canine); 102138971 (Cynomolgus Monkey); 100034196 (Equine); 493687 (Feline); 463288 (Primate); 100008733 (Rabbit)
Alternate Names
B-cell differentiation factor; B-cell stimulatory factor 2; BSF2; BSF-2; CDF; CTL differentiation factor ; HSF; hybridoma growth factor; IFNB2; IFN-beta-2; IL6; IL-6; Interferon beta-2; interleukin 6 (interferon, beta 2); interleukin BSF-2; interleukin-6; MGI-2A

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Citations for Human IL-6 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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  1. Reciprocal Functional Modulation of the Activation of T Lymphocytes and Fibroblasts Derived from Human Solid Tumors
    Authors: Jennifer L. Barnas, Michelle R. Simpson-Abelson, Stephen P. Brooks, Raymond J. Kelleher, Richard B. Bankert
    The Journal of Immunology
  2. Features of Postoperative Immune Suppression Are Reversible With Interferon Gamma and Independent of Interleukin-6 Pathways
    Authors: E. Rebecca Longbottom, Hew D. T. Torrance, Helen C. Owen, Paraskevi C. Fragkou, Charles J. Hinds, Rupert M. Pearse et al.
    Annals of Surgery
  3. A translational approach to micro-inflammation in end-stage renal disease: molecular effects of low levels of interleukin-6
    Authors: Bruno Memoli, Simona Salerno, Alfredo Procino, Loredana Postiglione, Sabrina Morelli, Maria Luisa Sirico et al.
    Clinical Science
  4. Cystathionine-beta -synthase is essential for AKT-induced senescence and suppresses the development of gastric cancers with PI3K/AKT activation
    Authors: Haoran Zhu, Keefe T Chan, Xinran Huang, Carmelo Cerra, Shaun Blake, Anna S Trigos et al.
    eLife
  5. IL6 is associated with response to dasatinib and cetuximab: Phase II clinical trial with mechanistic correlatives in cetuximab-resistant head and neck cancer
    Authors: L.P. Stabile, A.M. Egloff, M.K. Gibson, W.E. Gooding, J. Ohr, P. Zhou et al.
    Oral Oncology
  6. Early growth response 2 (EGR2) is a novel regulator of the senescence programme
    Authors: Eleanor J. Tyler, Ana Gutierrez del Arroyo, Bethany K. Hughes, Ryan Wallis, James C. Garbe, Martha R. Stampfer et al.
    Aging Cell
  7. Treatment of type 2 diabetes with the designer cytokine IC7Fc
    Authors: M Findeisen, TL Allen, DC Henstridge, H Kammoun, AE Brandon, LL Baggio, KI Watt, M Pal, L Cron, E Estevez, C Yang, GM Kowalski, L O'Reilly, C Egan, E Sun, LM Thai, G Krippner, TE Adams, RS Lee, J Grötzinger, C Garbers, S Risis, MJ Kraakman, NA Mellet, J Sligar, ET Kimber, RL Young, MA Cowley, CR Bruce, PJ Meikle, PA Baldock, P Gregorevic, TJ Biden, GJ Cooney, DJ Keating, DJ Drucker, S Rose-John, MA Febbraio
    Nature, 2019-09-25;574(7776):63-68.
    Species: Mouse
    Sample Types: Whole Blood
    Applications: ELISA Capture
  8. Small Extracellular Vesicles Are Key Regulators of Non-cell Autonomous Intercellular Communication in Senescence via the Interferon Protein IFITM3
    Authors: M Borghesan, J Fafián-Lab, O Eleftheria, P Carpintero, M Paez-Ribes, G Vizcay-Bar, A Swisa, D Kolodkin-G, P Ximénez-Em, R Lowe, B Martín-Mar, H Peinado, J Muñoz, RA Fleck, Y Dor, I Ben-Porath, A Vossenkamp, D Muñoz-Espi, A O'Loghlen
    Cell Rep, 2019-06-25;27(13):3956-3971.e6.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. Leukemia inhibitory factor functions in parallel with interleukin-6 to promote ovarian cancer growth
    Authors: K McLean, L Tan, DE Bolland, LG Coffman, LF Peterson, M Talpaz, N Neamati, RJ Buckanovic
    Oncogene, 2018-10-10;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  10. Autophagy facilitates TLR4- and TLR3-triggered migration and invasion of lung cancer cells through the promotion of TRAF6 ubiquitination.
    Authors: Zhan Z, Xie X, Cao H, Zhou X, Zhang X, Fan H, Liu Z
    Autophagy, 2013-12-04;10(2):257-68.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  11. Adipocytes promote ovarian cancer metastasis and provide energy for rapid tumor growth.
    Authors: Nieman KM, Kenny HA, Penicka CV, Ladanyi A, Buell-Gutbrod R, Zillhardt MR, Romero IL, Carey MS, Mills GB, Hotamisligil GS, Yamada SD, Peter ME, Gwin K, Lengyel E
    Nat. Med., 2011-10-30;17(11):1498-503.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  12. CD40-ligand stimulates myelopoiesis by regulating flt3-ligand and thrombopoietin production in bone marrow stromal cells.
    Authors: Solanilla A, Dechanet J, El Andaloussi A, Dupouy M, Godard F, Chabrol J, Charbord P, Reiffers J, Nurden AT, Weksler B, Moreau JF, Ripoche J
    Blood, 2000-06-15;95(12):3758-64.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  13. Human Herpesvirus 8 Viral Interleukin-6 Interacts with Splice Variant 2 of Vitamin K Epoxide Reductase Complex Subunit 1
    Authors: Daming Chen, Emily Cousins, Gordon Sandford, John Nicholas
    Journal of Virology
  14. Soluble factors produced by activated CD4+ T cells modulate EBV latency
    Authors: Nagy N, Adori M, Rasul A et al.
    Proc Natl Acad Sci U S A
  15. STAT3 Induction of miR-146b Forms a Feedback Loop to Inhibit the NF-kappa B to IL-6 Signaling Axis and STAT3-Driven Cancer Phenotypes
    Authors: Michael Xiang, Nicolai J. Birkbak, Vida Vafaizadeh, Sarah R. Walker, Jennifer E. Yeh, Suhu Liu et al.
    Science Signaling

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