Human IL-6 Magnetic Luminex® Performance Assay
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Human IL-6 Magnetic Luminex® Performance Assay Summary
| Panel | Luminex Performance Human Cytokine Panel A Luminex Performance Human Obesity Panel |
| Assay Type | Magnetic bead-based multiplex assay for the Luminex® platform |
| Format | 1 x 96-well microplate and Magnetic antibody-coated beads |
| Species | Human |
| Analytes Detected | Please see analyte list in assay customization tool below. |
| Performance Validation | Our Luminex High Performance Assays undergo our most extensive validation testing. All Luminex High Performance Assay Panels are validated for use with serum and plasma samples. Some High Performance Assays are additionally validated for cell culture supernatants, milk, saliva, or urine, as indicated on the individual product data sheets. Luminex High Performance Assays are tested for sensitivity (three-quarters the low standard), intra-assay precision, inter-assay precision, and to ensure assay linearity for validated sample types. Recovery values for individual samples in validated sample types are also tested. Validation data for each analyte can be found in the product datasheet. |
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Magnetic Luminex High Performance Assays are flexible bead-based multiplex assays. They allow up to 50 user-defined target analytes to be simultaneously profiled using cell culture supernates, serum, or plasma samples. Some panels are also validated with human milk, saliva, and/or urine samples.
- Simultaneously profile Human IL-6 with up to analytes of your choice in one sample
- Magnetic format allows for easier wash steps
- Undergoes similar validation testing as Quantikine® ELISA Kits
- Precise and Specific
- Requires a small sample volume (<50 µL)
- Run your assay in just 3-5 hours
- Mass-calibrated standards for consistent results with every new lot of material
- Enough reagents for one 96-well plate
- Standard Cocktail(s)
- Bead Diluent
- Biotin Antibody Diluent
- Calibrator Diluent
- Wash Buffer
- Streptavidin-PE
- One flat-bottom 96-well Microplate
- Foil Plate Sealers (4)
- Mixing Bottle
- Standard Value Card**
**A standard curve must be generated each time an assay is run, utilizing values from the Standard Value Card included in the Base Kit.
- User-mix antibody-coated Magnetic beads in individual vials
- User-mixed cocktail of biotinylated detection antibody
Precision
|
Intra-assay Precision (precision within an assay) - Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. |
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| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 25 | 25 | 25 |
| Mean (pg/mL) | 30.2 | 180 | 903 | 34 | 189 | 900 |
| Standard deviation | 1.43 | 7.78 | 41.9 | 3.00 | 13.0 | 53.0 |
| CV (%) | 4.7 | 4.3 | 4.6 | 7.9 | 6.7 | 5.9 |
Recovery
|
Samples containing and/or spiked with human IL-6 were evaluated for recovery. |
||||||
| Sample Type | Average % Recovery | Range (%) | ||||
|---|---|---|---|---|---|---|
| Cell culture supernate | 96 | 91-100 | ||||
| Serum | 108 | 95-118 | ||||
| EDTA plasma | 102 | 95-116 | ||||
| Heparin plasma | 109 | 96-129 | ||||
Linearity
|
Samples containing and/or spiked with human IL-6 were serially diluted to evaluate assay linearity. |
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| Cell culture supernate | Serum | EDTA plasma | Heparin plasma | ||||||
|---|---|---|---|---|---|---|---|---|---|
| 1:2 | Average % of Expected | 101 | 106 | 103 | 99 | ||||
| Range (%) | 87-117 | 104-109 | 82-124 | 86-110 | |||||
| 1:4 | Average % of Expected | 103 | 112 | 122 | 101 | ||||
| Range (%) | 86-127 | 103-117 | 116-129 | 85-114 | |||||
| 1:8 | Average % of Expected | 102 | 115 | 126 | 100 | ||||
| Range (%) | 82-130 | 97-126 | 121-131 | 87-110 | |||||
Assays for the Luminex platform are offered as High Performance Assays or Assays. The Luminex High Performance Assays are fully validated panels with a focused selection of analytes. They are available in We Mix, You Mix, and Predetermined formats. The Luminex Assays allows for the maximum number of analytes in a multiplex and is supplied as a premixed kit. View a table comparing the features and benefits of these bead-based multiplex assays.
Magnetic Luminex High Performance Assays are designed for use with the Luminex MAGPIX CCD Imager. Alternatively, kits can be used with the Luminex 100/200™ or FLEXMAP 3D, dual laser, flow-based sorting and detection platforms.
Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards, and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest. After washing away any unbound substances, a biotinylated antibody cocktail specific to the analytes of interest is added to each well. Following a wash to remove any unbound biotinylated antibody, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE), which binds to the biotinylated detection antibodies, is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using the Luminex or Bio-Plex analyzer. One laser is bead-specific and determines which analyte is being detected. A magnet in the analyzer captures and holds the superparaMagnetic microparticles in a monolayer. Two spectrally distinct Light Emitting Diodes (LEDs) illuminate the beads. One LED identifies the analyte that is being detected and the second LED determines the magnitude of the PE-derived signal, which is in direct proportion to the amount of analyte bound. Each well is imaged with a CCD camera. Kits can also be used with Luminex 100/200 or a Bio-Rad Bio-Plex dual laser, flow-based systems.
Scientific Data
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Quantist software graph of signal intensity versus concentration for IL-6 in the Human Cytokine A Luminex Panel
Background: IL-6
IL-6 (Interleukin-6) is a pleiotropic cytokine that acts in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. It contributes to chronic inflammation in obesity, insulin resistance, inflammatory bowel disease, arthritis, sepsis, and atherosclerosis. IL-6 signals through a receptor complex of IL-6 R alpha and gp130. gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130-expressing cells that lack cell surface IL-6 R alpha.
Citations for Human IL-6 Magnetic Luminex® Performance Assay
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
12
Citations: Showing 1 - 10
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Insulin Resistance in Women Correlates with Chromatin Histone Lysine Acetylation, Inflammatory Signaling, and Accelerated Aging
Authors: Vidal, CM;Alva-Ornelas, JA;Chen, NZ;Senapati, P;Tomsic, J;Robles, VM;Resto, C;Sanchez, N;Sanchez, A;Hyslop, T;Emwas, N;Aljaber, D;Bachelder, N;Martinez, E;Ann, D;Jones, V;Winn, RA;Miele, L;Ochoa, AC;Dietze, EC;Natarajan, R;Schones, D;Seewaldt, VL;
Cancers
Species: Human
Sample Types: Serum
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Coronary Artery Disease in Persons With Human Immunodeficiency Virus Without Detectable Viral Replication
Authors: Knudsen, AD;Fuchs, A;Benfield, T;Gerstoft, J;Køber, L;Trøseid, M;Kofoed, KF;Nielsen, SD;
Open forum infectious diseases
Species: Human
Sample Types: Plasma
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25-hydroxycholesterol exacerbates vascular leak during acute lung injury
Authors: JH Madenspach, ED Morrell, JG McDonald, BM Thompson, Y Li, KG Birukov, AA Birukova, RD Stapleton, A Alejo, PW Karmaus, JM Meacham, P Rai, C Mikacenic, MM Wurfel, MB Fessler
JCI Insight, 2023-04-10;0(0):.
Species: Human
Sample Types: BALF
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SARS-CoV-2 Infection Modulates ACE2 Function and Subsequent Inflammatory Responses in Swabs and Plasma of COVID-19 Patients
Authors: L Gutiérrez-, E Riveira-Mu, C Barrios, V Palau, M Nevot, S Pedreño-Ló, J Senserrich, M Massanella, B Clotet, C Cabrera, O Mitjà, M Crespo, J Pascual, M Riera, E Ballana
Viruses, 2021-08-28;13(9):.
Species: Human
Sample Types: Plasma
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IRE1 Alpha/XBP1 Axis Sustains Primary Effusion Lymphoma Cell Survival by Promoting Cytokine Release and STAT3 Activation
Authors: R Gonnella, MS Gilardini, L Guttieri, MA Romeo, R Santarelli, M Cirone
Biomedicines, 2021-01-27;9(2):.
Species: Human
Sample Types: Cell Culture Supernates
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Disharmonic Inflammatory Signatures in COVID-19: Augmented Neutrophils' but Impaired Monocytes' and Dendritic Cells' Responsiveness
Authors: Z Parackova, I Zentsova, M Bloomfield, P Vrabcova, J Smetanova, A Klocperk, G Mesežnikov, LF Casas Mend, T Vymazal, A Sediva
Cells, 2020-09-29;9(10):.
Species: Human
Sample Types: Cell Culture Supernates
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Endo-1,3(4)-beta-Glucanase-Treatment of Oat beta-Glucan Enhances Fermentability by Infant Fecal Microbiota, Stimulates Dectin-1 Activation and Attenuates Inflammatory Responses in Immature Dendritic Cells
Authors: R Akkerman, MJ Logtenberg, R An, MA Van Den Be, BJ de Haan, MM Faas, E Zoetendal, P de Vos, HA Schols
Nutrients, 2020-06-03;12(6):.
Species: Human
Sample Types: Cell Culture Supernates
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Candidalysin activates innate epithelial immune responses via epidermal growth factor receptor
Authors: J Ho, X Yang, SA Nikou, N Kichik, A Donkin, NO Ponde, JP Richardson, RL Gratacap, LS Archambaul, CP Zwirner, C Murciano, R Henley-Smi, S Thavaraj, CJ Tynan, SL Gaffen, B Hube, RT Wheeler, DL Moyes, JR Naglik
Nat Commun, 2019-05-24;10(1):2297.
Species: Human
Sample Types: Cell Culture Supernates
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The importance of inter-individual Kupffer cell variability in the governance of hepatic toxicity in a 3D primary human liver microtissue model
Authors: A Kermanizad, DM Brown, W Moritz, V Stone
Sci Rep, 2019-05-13;9(1):7295.
Species: Human
Sample Types: Cell Culture Supernates
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A ferroptosis-based panel of prognostic biomarkers for Amyotrophic Lateral Sclerosis
Authors: D Devos, C Moreau, M Kyheng, G Garçon, AS Rolland, H Blasco, P Gelé, T Timothée L, C Veyrat-Dur, P Corcia, M Dutheil, P Bede, A Jeromin, P Oeckl, M Otto, V Meninger, V Danel-Brun, JC Devedjian, JA Duce, PF Pradat
Sci Rep, 2019-02-27;9(1):2918.
Species: Human
Sample Types: Plasma
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A randomised study in young subjects of the effects of eating extra fruit or nuts on periodontal inflammation
BDJ Open, 2018-02-09;0(0):.
Species: Human
Sample Types: Cell Culture Supernates
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Processing of Candida albicans Ece1p Is Critical for Candidalysin Maturation and Fungal Virulence
Authors: JP Richardson, S Mogavero, DL Moyes, M Blagojevic, T Krüger, AH Verma, BM Coleman, J De La Cruz, D Schulz, NO Ponde, G Carrano, O Kniemeyer, D Wilson, O Bader, SI Enoiu, J Ho, N Kichik, SL Gaffen, B Hube, JR Naglik
MBio, 2018-01-23;9(1):.
Species: Human
Sample Types: Cell Culture Supernates
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IL-6 performed as expected with Low % CVs
