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Human IL‑7R alpha /CD127 Biotinylated Antibody

Catalog #: BAF306
10 Citations Datasheet / COA / SDS
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BAF306
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Citations (10)
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Human IL‑7R alpha /CD127 Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-7 R alpha in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen

Mouse myeloma cell line NS0-derived recombinant human IL-7 R alpha


Extracellular domain
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human IL‑7 R alpha /CD127 Fc Chimera (Catalog # 306-IR)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-7R alpha/CD127

Interleukin 7 Receptor alpha (IL-7 R alpha ), also known as CD127, is a 75 kDa hematopoietin receptor superfamily member that plays an important role in lymphocyte differentiation, proliferation, and survival (1, 2). Mature human IL-7 R alpha consists of a 219 amino acid (aa) extracellular domain (ECD) with one fibronectin type-III domain and a WSXWS motif, a 25 aa transmembrane segment, and a 195 aa cytoplasmic domain (3). Alternate splicing of human IL-7 R alpha generates a secreted soluble form of the receptor (3). Within the ECD, human IL‑7 R alpha shares 67% aa sequence identity with mouse and rat IL-7 R alpha. IL-7 R alpha associates with the common gamma chain ( gamma c) to form the functional high affinity IL-7 receptor complex (4). The gamma c is also a subunit of the receptors for IL-2, -4, -9, -15, and -21. Human and mouse IL‑7 show cross-species activity through the IL-7 receptor (3, 5). IL-7 R alpha is expressed on double negative (CD4-/CD8-) and CD4+ or CD8+ single positive T cells as well as on CD8+ memory T cells and their precursors (6, 7). It is expressed early in B cell development, prior to the appearance of surface IgM (6). In mouse, IL-7 activation of IL-7 R alpha is critical for both T cell and B cell lineage development (8). In human, by contrast, it is required for T cell but not for B cell development (9). IL-7 induces the downregulation and shedding of cell surface IL‑7 R alpha (10). IL-7 R alpha additionally associates with TSLP R to form the functional receptor for thymic stromal lymphopoietin (11, 12). TSLP indirectly regulates T cell development by modulating dendritic cell activation (2, 13). Knockout of TSLP R in mice provokes minor changes in B and T cell development compared to those seen with IL-7 R alpha deletion (8, 14). The complexity of IL-7 R alpha biology is suggested by the competition between IL-7 and TSLP for receptor binding and by the ability of IL-7 R alpha to form functional complexes with SCF R and HGF R (11, 12, 15, 16).

References
  1. Mazzucchelli, R. and S.K. Durum (2007) Nat. Rev. Immunol. 7:144.
  2. Liu, Y.-J. et al. (2007) Annu. Rev. Immunol. 25:193.
  3. Goodwin, R.G. et al. (1990) Cell 60:941.
  4. Noguchi, M. et al. (1993) Science 262:1877.
  5. Barata, J.T. et al. (2006) Exp. Hematol. 34:1133.
  6. Sudo, T. et al. (1993) Proc. Natl. Acad. Sci. 90:9125.
  7. Kaech, S.M. et al. (2003) Nat. Immunol. 4:1191.
  8. Peschon, J.J. et al. (1994) J. Exp. Med. 180:1955.
  9. Prieyl, J.A. and T.W. LeBien (1996) Proc. Natl. Acad. Sci. 93:10348.
  10. Vranjkovic, A. et al. (2007) Int. Immunol. 19:1329.
  11. Park, L.S. et al. (2000) J. Exp. Med. 192:659.
  12. Pandey, A. et al. (2000) Nat. Immunol. 1:59.
  13. Reche, P.A. et al. (2001) J. Immunol. 167:336.
  14. Al-Shami, A. et al. (2004) J. Exp. Med. 200:159.
  15. Jahn, T. et al. (2007) Blood 110:1840.
  16. Lai, L. et al. (2006) Blood 107:1776.
Long Name
Interleukin 7 Receptor alpha
Entrez Gene IDs
3575 (Human); 16197 (Mouse); 294797 (Rat); 102143679 (Cynomolgus Monkey)
Alternate Names
CD127 antigen; CD127; CD127ILRA; CDW127; IL-7 R alpha; IL-7 receptor subunit alpha; IL7R alpha; IL-7R alpha; IL-7R subunit alpha; IL7R; IL7RA; IL-7Ra; IL-7R-alpha; interleukin 7 receptor alpha chain; interleukin 7 receptor isoform H5-6; interleukin 7 receptor; interleukin-7 receptor subunit alpha

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Citations for Human IL‑7R alpha /CD127 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Antisense modulation of IL7R splicing to control sIL7R expression in human CD4+ T cells
    Authors: Gaddiel Galarza-Muñoz, Debbie Kennedy-Boone, Geraldine Schott, Shelton S. Bradrick, Mariano A. Garcia-Blanco
    RNA
  2. Interleukin-7 dependent non-classical monocytes and CD40 expression are affected in children with type 1 diabetes
    Authors: P Hehenkamp, M Hoffmann, S Kummer, C Reinauer, C Döing, K Förtsch, J Enczmann, V Balz, E Mayatepek, T Meissner, M Jacobsen, J Seyfarth
    European Journal of Immunology, 2021-11-01;0(0):.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Detection
  3. IL-7 induces sCD127 release and mCD127 downregulation in human CD8+ T cells by distinct yet overlapping mechanisms, both of which are impaired in HIV infection
    Authors: SC Côté, SCB Schinkel, TK Berthoud, PO Barros, M Sanchez-Vi, AM Davidson, AM Crawley, JB Angel
    Eur. J. Immunol., 2020-05-27;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates, Whole Cells
    Applications: ELISA Detection, Flow Cytometry
  4. Context-specific regulation of surface and soluble IL7R expression by an autoimmune risk allele
    Authors: H Al-Mossawi, N Yager, CA Taylor, E Lau, S Danielli, J de Wit, J Gilchrist, I Nassiri, EA Mahe, W Lee, L Rizvi, S Makino, J Cheeseman, M Neville, JC Knight, P Bowness, BP Fairfax
    Nat Commun, 2019-10-08;10(1):4575.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Detection
  5. CD56(bright) NK IL-7R? expression negatively associates with HCV level, and IL-7-induced NK function is impaired during HCV and HIV infections
    Authors: CJ Judge, L Kostadinov, KE Sherman, AA Butt, Y Falck-Ytte, NT Funderburg, AL Landay, MM Lederman, SF Sieg, JK Sandberg, DD Anthony
    J. Leukoc. Biol., 2017-04-11;0(0):.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development
  6. Reduced plasma levels of soluble interleukin-7 receptor during graft-versus-host disease (GVHD) in children and adults.
    Authors: Poiret, Thomas, Rane, Lalit, Remberger, Mats, Omazic, Birgitta, Gustafsson-Jernberg, Asa, Vudattu, Nalini K, Ahmed, Raija, Ernberg, Ingemar, Winiarski, Jacek, Magalhaes, Isabelle, Ringden, Olle, Maeurer, Markus
    BMC Immunol, 2014-06-19;15(0):25.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development (Detection)
  7. IL-7 Restores Lymphocyte Functions in Septic Patients.
    Authors: Venet F, Foray A, Villars-Mechin A, Malcus C, Poitevin-Later F, Lepape A, Monneret G
    J Immunol, 2012-10-10;189(10):5073-81.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  8. Decreased systemic IL-7 and soluble IL-7Ralpha in multiple sclerosis patients.
    Genes Immun, 2012-08-23;13(7):587-92.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  9. Functionally significant differences in expression of disease-associated IL-7 receptor alpha haplotypes in CD4 T cells and dendritic cells.
    Authors: Hoe E, McKay FC, Schibeci SD, Gandhi K, Heard RN, Stewart GJ, Booth DR
    J. Immunol., 2010-01-22;184(5):2512-7.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development
  10. Identification and biochemical characterization of human plasma soluble IL-7R: lower concentrations in HIV-1-infected patients.
    Authors: Rose T, Lambotte O, Pallier C, Delfraissy JF, Colle JH
    J. Immunol., 2009-06-15;182(12):7389-97.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development

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