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Human IL-9 Antibody

Catalog #: AB-209-NA
4 Citations Datasheet / COA / SDS
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AB-209-NA
Cell Proliferation Induced by IL‑9 and Neutralization by Human IL‑9 Antibody.
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Citations (4)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-9 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-9 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 20% cross-reactivity with recombinant mouse IL-9 is observed.
Source
Polyclonal Goat IgG
Purification
Protein A or G purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-9
Gln19-Ile144
Accession # P15248
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human IL‑9 (Catalog # 209-IL)
Neutralization
Measured by its ability to neutralize IL‑9-induced proliferation in the MO7e human megakaryocytic leukemic cell line. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359. The Neutralization Dose (ND50) is typically 20-40 µg/mL in the presence of 5 ng/mL Recombinant Human IL‑9.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by IL‑9 and Neutralization by Human IL‑9 Antibody. View Larger

Cell Proliferation Induced by IL‑9 and Neutralization by Human IL‑9 Antibody. Recombinant Human IL‑9 (Catalog # 209-IL) stimulates proliferation in the MO7e human megakaryocytic leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑9 (5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‑9 Polyclonal Antibody (Catalog # AB-209-NA). The ND50 is typically 20-40 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 1 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-9

Human IL-9 was originally identified as a cytokine found in the conditioned medium of a human T cell leukemia virus type I (HTLV-I) transformed T cell line that is mitogenic for the factor-dependent human megakaryoblastic leukemic cell line, M07e. The cDNA encoding this cytokine was subsequently isolated by functional expression cloning and found to be similar to the mouse T cell growth factor III/P40. This human cytokine and its murine homologue are now designated as human and mouse IL-9. Besides HTLV-I or -II transformed T cell lines, rhIL-9 is also produced by activated human PBLs. Human IL-9 was also reported to be expressed by primary and cultured Hodgkin and Reed-Sternberg (H-RS) cells derived from Hodgkin’s disease patients, suggesting a possible role for rhIL-9 in the development of the pathophysiology of Hodgkin’s disease.

Human and murine IL-9 are also capable of enhancing in vitro survival of human T cell lines as well as synergizing with Epo to support erythroid colony formation in vitro. However, the mast cell enhancing activity associated with rmIL-9 has not yet been demonstrated in the human system and no human IL-9-dependent T cell clones have been identified.

The gene for rhIL-9 has been mapped to human chromosome 5. As in the mouse system, the human IL-9 cDNA encodes a 144 amino acid residue precursor protein with an 18 amino acid signal peptide that is cleaved to form the mature cysteine-rich protein with a predicted molecular mass of 14 kDa. Human IL-9 contains four potential N-linked glycosylation sites and the native rhIL-9 is a highly glycosylated protein. Human and mouse IL-9 share 56% and 67% homology at the amino acid and nucleotide levels, respectively. Although murine IL-9 is active on human cells, human IL-9 is not active on mouse cells.

Long Name
Interleukin 9
Entrez Gene IDs
3578 (Human); 16198 (Mouse)
Alternate Names
Cytokine P40; HP40; IL9; IL-9; IL-9homolog of mouse T cell and mast cell growth factor 40; interleukin 9; interleukin-9; p40 cytokine; p40 T-cell and mast cell growth factor; P40; T-cell growth factor P40

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Citations for Human IL-9 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Cloning and functional testing of rhesus macaque ( Macaca mulatta ) IL‐9 and IL‐33
    Authors: Sanghita Sarkar, Ann J. Hessell, Nancy L. Haigwood, James J. Kobie
    Journal of Medical Primatology
  2. STAT3/5-Dependent IL9 Overexpression Contributes to Neoplastic Cell Survival in Mycosis Fungoides
    Authors: Pablo A. Vieyra-Garcia, Tianling Wei, David Gram Naym, Simon Fredholm, Regina Fink-Puches, Lorenzo Cerroni et al.
    Clinical Cancer Research
  3. IL-9 regulates allergen-specific Th1 responses in allergic contact dermatitis.
    Authors: Liu J, Harberts E, Tammaro A, Girardi N, Filler R, Fishelevich R, Temann A, Licona-Limon P, Girardi M, Flavell R, Gaspari A
    J Invest Dermatol, 2014-01-31;134(7):1903-11.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  4. Autocrine release of interleukin-9 promotes Jak3-dependent survival of ALK+ anaplastic large-cell lymphoma cells.
    Authors: Qiu L, Lai R, Lin Q, Lau E, Thomazy DM, Calame D, Ford RJ, Kwak LW, Kirken RA, Amin HM
    Blood, 2006-06-08;108(7):2407-15.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells, Whole Tissue
    Applications: ICC, IHC-P, Neutralization, Western Blot

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