Human ILT1/CD85h APC-conjugated Antibody

Catalog #: FAB6364A Datasheet / COA / SDS

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FAB6364A

Detection of ILT1/CD85h in Human Blood Monocytes by Flow Cytometry.

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Human ILT1/CD85h APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human ILT1/CD85h in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) ILT2, 3, 4, 5, rhLIR6, or rhLIR8 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 600007

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human ILT1/CD85h
Pro17-Ser437
Accession # Q8N149

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of ILT1/CD85h in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human ILT1/CD85h APC-conjugated Monoclonal Antibody (Catalog # FAB6364A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: LILRA2/CD85h/ILT1

Immune-Like Transcript 1 (ILT1), also known as Leukocyte Immunoglobulin-like Receptor 7 (LIR-7), Leukocyte Immunoglobulin-like Receptor subfamily A member 2 (LILRA2), and CD85h, is a 51 kDa (unglycosylated) type I transmembrane glycoprotein and member of the leukocyte immunoglobulin-like receptor family. Human ILT1 is synthesized as a 483 amino acid (aa) precursor that contains a 23 aa signal sequence, a 426 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 13 aa cytoplasmic region. The ECD contains four Ig-like C2-type domains and seven potential sites for N-linked glycosylation. A splice variant produces a second isoform that has a one aa substitution for aa 419-436 in the longer form. There are no murine orthologs for human ILT1. ILT1 may act as a receptor for class I MHC antigens.

Long Name

Leukocyte Immunoglobulin-like Receptor, Subfamily A (with TM domain), Member 2

Entrez Gene IDs

11027 (Human)

Alternate Names

CD85 antigen-like family member H; CD85h antigen; CD85h; ILT1; ILT-1; ILT1CD85H; Leukocyte immunoglobulin-like receptor 7; leukocyte immunoglobulin-like receptor subfamily A member 2 soluble; leukocyte immunoglobulin-like receptor subfamily A member 2; leukocyte immunoglobulin-like receptor, subfamily A (with TM domain), member 2; LILRA2; LIR7; LIR7LIR-7Immunoglobulin-like transcript 1