Human Indoleamine 2,3-dioxygenase/IDO Antibody

Catalog #: MAB104981 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB104981-100
MAB104981-SP

Human Indoleamine 2,3‑dioxygenase/IDO ELISA Standard Curve.

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All Indoleamine 2,3-dioxygenase/IDO Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Indoleamine 2,3-dioxygenase/IDO Antibody Summary

Species Reactivity

Human

Specificity

Detects human Indoleamine 2,3‑dioxygenase/IDO in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 700826

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Indoleamine 2,3‑dioxygenase/IDO
Ala2-Gly403
Accession # P14902

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human Indoleamine 2,3‑dioxygenase/IDO Monoclonal Antibody (Catalog # MAB10498).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Indoleamine 2,3-dioxygenase/IDO DuoSet ELISA Kit (Catalog # DY6030-05) for convenient development of a sandwich ELISA.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA

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Human Indoleamine 2,3‑dioxygenase/IDO ELISA Standard Curve. Recombinant Human Indoleamine 2,3‑dioxygenase/IDO protein was serially diluted 2-fold and captured by Mouse Anti-Human Indoleamine 2,3‑dioxygenase/IDO Monoclonal Antibody (MAB10498) coated on a Clear Polystyrene Microplate (DY990). Mouse Anti-Human Indoleamine 2,3‑dioxygenase/IDO Monoclonal Antibody (Catalog # MAB104981) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994 ).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Indoleamine 2,3-dioxygenase/IDO

Indoleamine 2,3-dioxygenase (IDO) is a heme-containing intracellular dioxygenase catalyzing the degradation of the essential amino acid L-tryptophan to N-formyl-kynurenine (1). This degradation is the first and rate-limiting step of the L-kynurenine pathway (2). IDO is widely expressed in dendritic cells, macrophages, microglia, eosinophils, fibroblasts, endothelial cells, and most tumor cells. In immune cells, its expression is mainly induced by cytokines such as IFN-gamma, IFN-alpha, IFN-beta, and IL‑10. IDO has an antimicrobial function due to its decreasing the availability of the essential amino acid tryptophan in inflammatory environments (3). Recent studies have demonstrated that IDO induces immunosuppression during infection, pregnancy, transplantation, autoimmunity, and neoplasia (3-5).

References

Lewis-Ballester, A. et al. (2009) Proc. Natl. Acad. Sci. USA. 106:17371.
Costantino, G. (2009) Expert Opin. Ther. Targets 13:247.
Xu, H. et al. (2008) Immunol. Lett. 121:1.
Lob, S. et al. (2009) Nat. Rev. Cancer 9:445.
Curti, A. et al. (2009) Blood 113:2394.

Entrez Gene IDs

3620 (Human); 15930 (Mouse)

Alternate Names

3dioxygenase; EC 1.13.11.52; IDO; IDO1; IDOIDO-1; INDO; INDOindole 2,3-dioxygenase; Indoleamine 2; indoleamine 2,3-dioxygenase 1; Indoleamine 2,3-dioxygenase; indoleamine-pyrrole 2,3 dioxygenase; Indoleamine-pyrrole 2,3-dioxygenase