Human Indoleamine 2,3-dioxygenase/IDO APC-conjugated Antibody
Human Indoleamine 2,3-dioxygenase/IDO APC-conjugated Antibody Summary
Ala2-Gly403
Accession # P14902
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Indoleamine 2,3‑dioxygenase/IDO in Human Monocytes by Flow Cytometry. Human Monocytes were selected from PBMC using MagCellect Human CD14+ Cell Isolation Kit (MAGH105) and cultured overnight with Recombinant Human MCSF (50 ng/mL; 216-MC), Recombinant Human IFN gamma (50 ng/mL; 285-IF) and 50 ng/mL LPS and stained with (A) Mouse Anti-Human Indoleamine 2,3-dioxygenase/IDO APC-conjugated Monoclonal Antibody (Catalog # IC6030A) or (B) Mouse IgG1 isotype control antibody (IC002A) and Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (FAB3832P). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Perm Kit (FC012). Staining was performed using our Staining Intracellular Molecules protocol.
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Detection of Indoleamine 2,3‑dioxygenase/IDO in Human MDSCs by Flow Cytometry. Human PBMC-derived myeloid-derived suppressor cells (MDSCs) treated with 10 ng/mL Recombinant Human IL-6 (Catalog # 206-IL) and 10 ng/mL Recombinant Human GM-CSF (Catalog # 215-GM) for 7 days were stained with Mouse Anti-Human Siglec-3/CD33 PE-conjugated Monoclonal Antibody (Catalog # FAB1137P) and either (A) Mouse Anti-Human Indoleamine 2,3-dioxygenase/IDO APC-conjugated Monoclonal Antibody (Catalog # IC6030A) or (B) Mouse IgG1Allophycocyanin Isotype Control (Catalog # IC002A). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Indoleamine 2,3-dioxygenase/IDO
Indoleamine 2,3-dioxygenase (IDO) is a heme-containing intracellular dioxygenase catalyzing the degradation of the essential amino acid L-tryptophan to N-formyl-kynurenine (1). This degradation is the first and rate-limiting step of the L-kynurenine pathway (2). IDO is widely expressed in dendritic cells, macrophages, microglia, eosinophils, fibroblasts, endothelial cells, and most tumor cells. In immune cells, its expression is mainly induced by cytokines such as IFN-gamma, IFN-alpha, IFN-beta, and IL‑10. IDO has an antimicrobial function due to its decreasing the availability of the essential amino acid tryptophan in inflammatory environments (3). Recent studies have demonstrated that IDO induces immunosuppression during infection, pregnancy, transplantation, autoimmunity, and neoplasia (3-5).
- Lewis-Ballester, A. et al. (2009) Proc. Natl. Acad. Sci. USA. 106:17371.
- Costantino, G. (2009) Expert Opin. Ther. Targets 13:247.
- Xu, H. et al. (2008) Immunol. Lett. 121:1.
- Lob, S. et al. (2009) Nat. Rev. Cancer 9:445.
- Curti, A. et al. (2009) Blood 113:2394.
Product Datasheets
Citations for Human Indoleamine 2,3-dioxygenase/IDO APC-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Phosphonate-Modified Cellulose Nanocrystals Potentiate the Th1 Polarising Capacity of Monocyte-Derived Dendritic Cells via GABA-B Receptor
Authors: M Beki?, M Vasiljevi?, D Stojanovi?, V Kokol, D Mihajlovi?, D Vu?evi?, P Uskokovi?, M ?oli?, S Tomi?
International Journal of Nanomedicine, 2022-07-23;17(0):3191-3216.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Influence of Indoleamine-2,3-Dioxygenase and Its Metabolite Kynurenine on gammadelta T Cell Cytotoxicity against Ductal Pancreatic Adenocarcinoma Cells
Authors: H Jonescheit, HH Oberg, D Gonnermann, M Hermes, V Sulaj, C Peters, D Kabelitz, D Wesch
Cells, 2020-05-06;9(5):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Trichinella spiralis Excretory-Secretory Products Induce Tolerogenic Properties in Human Dendritic CellsviaToll-Like Receptors 2 and 4
Authors: N Ilic, A Gruden-Mov, J Cvetkovic, S Tomic, DB Vucevic, C Aranzamend, M Colic, E Pinelli, L Sofronic-M
Front Immunol, 2018-01-24;9(0):11.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
1-Methyl-tryptophan attenuates regulatory T cells differentiation due to the inhibition of estrogen-IDO1-MRC2 axis in endometriosis
Cell Death Dis, 2016-12-01;7(12):e2489.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Monocyte Differentiation towards Protumor Activity Does Not Correlate with M1 or M2 Phenotypes
Authors: G Karina Chimal-Ram
J Immunol Res, 2016-06-08;2016(0):6031486.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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