Human Indoleamine 2,3-dioxygenase/IDO PerCP-conjugated Antibody

Catalog #: IC6030C Datasheet / COA / SDS

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IC6030C

Detection of Indoleamine 2,3‑dioxygenase/IDO in Human MDSCs by Flow Cytometry.

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Human Indoleamine 2,3-dioxygenase/IDO PerCP-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Indoleamine 2,3-dioxygenase/IDO in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 700838

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Indoleamine 2,3-dioxygenase/IDO
Ala2-Gly403
Accession # P14902

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

PerCP (Peridinin-chlorophyll Protein Complex) (Excitation= 482 and 564 nm, Emission= 675 nm)

Applications

Recommended Concentration

Sample

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of Indoleamine 2,3-dioxygenase/IDO antibody in Human MDSCs antibody by Flow Cytometry.

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Detection of Indoleamine 2,3‑dioxygenase/IDO in Human MDSCs by Flow Cytometry. Human PBMC-derived myeloid-derived suppressor cells (MDSCs) treated with 10 ng/mL Recombinant Human IL-6 (Catalog # 206-IL) and 10 ng/mL Recombinant Human GM-CSF (Catalog # 215-GM) for 7 days were stained with Mouse Anti-Human Siglec-3/CD33 PE-conjugated Monoclonal Antibody (Catalog # FAB1137P) and either (A) Mouse Anti-Human Indoleamine 2,3-dioxygenase/IDO PerCP-conjugated Monoclonal Antibody (Catalog # IC6030C) or (B) Mouse IgG₁Allophycocyanin Isotype Control (Catalog # IC002C). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Indoleamine 2,3-dioxygenase/IDO

Indoleamine 2,3-dioxygenase (IDO) is a heme-containing intracellular dioxygenase catalyzing the degradation of the essential amino acid L-tryptophan to N-formyl-kynurenine (1). This degradation is the first and rate-limiting step of the L-kynurenine pathway (2). IDO is widely expressed in dendritic cells, macrophages, microglia, eosinophils, fibroblasts, endothelial cells, and most tumor cells. In immune cells, its expression is mainly induced by cytokines such as IFN-gamma, IFN-alpha, IFN-beta, and IL‑10. IDO has an antimicrobial function due to its decreasing the availability of the essential amino acid tryptophan in inflammatory environments (3). Recent studies have demonstrated that IDO induces immunosuppression during infection, pregnancy, transplantation, autoimmunity, and neoplasia (3-5).

References

Lewis-Ballester, A. et al. (2009) Proc. Natl. Acad. Sci. USA. 106:17371.
Costantino, G. (2009) Expert Opin. Ther. Targets 13:247.
Xu, H. et al. (2008) Immunol. Lett. 121:1.
Lob, S. et al. (2009) Nat. Rev. Cancer 9:445.
Curti, A. et al. (2009) Blood 113:2394.

Entrez Gene IDs

3620 (Human); 15930 (Mouse)

Alternate Names

3dioxygenase; EC 1.13.11.52; IDO; IDO1; IDOIDO-1; INDO; INDOindole 2,3-dioxygenase; Indoleamine 2; indoleamine 2,3-dioxygenase 1; Indoleamine 2,3-dioxygenase; indoleamine-pyrrole 2,3 dioxygenase; Indoleamine-pyrrole 2,3-dioxygenase